Analysis of expression of prgX, a key negative regulator of the transfer of the Enterococcus faecalis pheromone-inducible plasmid pCF10

Journal of Molecular Biology

Volumn 297, Issue 4, 2000, Pages 861-875

  Bae, Taeok ^a   Clerc Bardin, Sylvie ^a   Dunny, Gary M ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Bacteria; Conjugation; Induction; Pheromone; Plasmid

Indexed keywords

MESSENGER RNA; PHEROMONE; PROTEIN CCF10; PROTEIN PRGX; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ENTEROCOCCUS FAECALIS; GENE ACTIVITY; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; MOLECULAR STABILITY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN INDUCTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ENTEROCOCCUS FAECALIS;

EID: 0034615783     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3628     Document Type: Article

References (39)

1. Regulation of transfer of the Enterococcus faecalis pheromone-responding plasmid pAD1: Temperature-sensitive transfer mutants and identification of a new regulatory determinant
2. Regulation of the Enterococcus faecalis pAD1-related sex pheromone response: Analyses of traD expression and its role in controlling conjugation functions
3. Activation of pheromone-inducible conjugation functions
4. Cloning and molecular analysis of genes affecting expression of binding substance, the recipient-encoded receptor(s) mediating mating aggregate formation in Enterococcus faecalis
5. Pheromone-inducible expression of an aggregation protein in Enterococcus faecalis requires interaction of a plasmid-encoded RNA with components of the ribosome
6. Sensitive detection of bacterial transcription initiation sites and differentiation from RNA processing sites in the pheromone-induced plasmid transfer system of Enterococcus faecalis
7. Pheromone cCF10 and plasmid pCF10-encoded regulatory molecules act post-transcriptionally to activate expression of downstream conjugation functions
8. Transcriptional analysis of a region of the Enterococcus faecalis plasmid pCF10 involved in positive regulation of conjugative transfer functions
9. Controlled gene expression systems for Lactococcus-lactis with the food-grade inducer nisin
10. Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis
11. Induced cell aggregation and mating in Streptococcus faecalis: Evidence for a bacterial sex pheromone
12. Direct stimulation of the transfer of antibiotic resistance by sex pheromones in Streptococcus faecalis
13. Induction of surface exclusion (entry exclusion) by Streptococcus faecalis sex pheromones: Use of monoclonal antibodies to identify an inducible surface antigen involved in the exclusion process
14. Pheromone-inducible conjugation in Enterococcus faecalis: Interbacterial and host-parasite chemical communication
15. Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: Comparison of induction level and promoter strength
16. Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating, DNA-binding TraA protein
17. Transcriptional antitermination
18. Identification and characterization of the genes of Enterococcus faecalis plasmid pCF10 involved in replication and in negative control of pheromone-inducible conjugation
19. Transcriptional antitermination in the bgl operon of E. coli is modulated by a specific RNA binding protein
20. In vitro analysis of the interaction between the FinO protein and FinP anti-sense RNA of F-like conjugative plasmids
21. Degradation of FinP antisense RNA from F-like plasmids: The RNA-binding protein, FinO, protects FinP from ribonuclease E
22. Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis
23. Broad geographical distribution of homologous erythromycin, kanamycin, and streptomycin resistance determinants among group D streptococci of human and animal origin
24. Quantitative nonradioactive CAT assays using fluorescent BODIPY 1-deoxychloramphenicol substrates
25. Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation
26. Ribosome regulation by the nascent peptide
27. Structure of cCF10, a peptide sex pheromone which induces conjugative transfer of the Streptococcus faecalis tetracycline resistance plasmid, pCF10
28. The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10
29. Molecular mechanism of peptide-specific pheromone signaling in Enterococcus faecalis: Functions of pheromone receptor TraA and pheromone-binding protein TraC encoded by plasmid pPD1
30. Regulation of the pAD1-encoded sex pheromone response in Enterococcus faecalis: Nucleotide sequence analysis of traA
31. Conjugative transfer of Enterococcus faecalis plasmid pAD1: Nucleotide sequence and transcriptional fusion analysis of a region involved in positive regulation
32. RNA and protein elements of E. coli and λ transcription antitermination complexes
33. Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function
34. Regulation of the pAD1-encoded sex pheromone response in Enterococcus faecalis: Expression of the positive regulator TraE1
35. The traA gene of the Enterocaccus faecalis conjugative plasmid pPD1 encodes a negative regulator for the pheromone response
36. Shuttle vectors containing a multiple cloning site and a lacZ alpha gene for conjugal transfer of DNA from Escherichia coli to gram-positive bacteria
37. Highly efficient protoplast transformation system for Streptococcus faecalis and a new Esherichia coli-S. faecalis shuttle vector