Regulation of the Enterococcus faecalis paD1-related sex pheromone response: Analyses of trad expression and its role in controlling conjugation functions

Molecular Microbiology

Volumn 30, Issue 2, 1998, Pages 381-392

  Bastos, Maria Do Carmo De Freire ^a,b   Tomita, Haruyoshi ^a   Tanimoto, Koichi ^a,c   Clewell, Don B ^a,d,e  

^a UNIVERSITY OF MICHIGAN   (United States)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c GUNMA UNIVERSITY   (Japan)

^d UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^e School of Dentistry

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; HEMOLYSIN; RNA; SEX PHEROMONE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIUM CONJUGATION; CHIMERA; DNA FLANKING REGION; ENTEROCOCCUS FAECALIS; GENE EXPRESSION REGULATION; HEAT SENSITIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REGULATORY MECHANISM; RNA SYNTHESIS; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION; TRANSCRIPTION TERMINATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HEMOLYSIN FACTORS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BIOSYNTHESIS; REGULATORY SEQUENCES, NUCLEIC ACID; SEX ATTRACTANTS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; POSIBACTERIA; STREPTOCOCCUS;

EID: 0345007912     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.01074.x     Document Type: Article

References (46)

1. An, F.Y., and Clewell, D.B. (1994) Characterization of the determinant (traB) encoding sex pheromone shutdown by the hemolysin/bacteriocin plasmid pAD1 in Enterococcus faecalis. Plasmid 31: 215-221.
2. An, F.Y., and Clewell, D.B. (1997) The origin of transfer (oriT) of the enterococcal, pheromone-responding, cytolysin plasmid pAD1 is located within the repA determinant. Plasmid 37: 87-94.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moor, D.D., Seidman, J.G., Smith, J.A., et al. (1987) Current Protocols in Molecular Biology. New York: John Wiley & Sons.
4. Bastos, M.C.F., Tanimoto, K., and Clewell, D.B. (1997) Regulation of transfer of the Enterococcus faecalis pheromone-responding plasmid pAD1: temperature-sensitive transfer mutants and identification of a new regulatory determinant, traD. J Bacteriol 179: 3250-3259.
5. Bensing, B.A., and Dunny, G.M. (1997) Pheromone-inducible expression of an aggregation protein in Enterococcus faecalis requires interaction of a plasmid-encoded RNA with components of the ribosome. Mol Microbiol 24: 295-308.
6. Bensing, B.A., Manias, D.A., and Dunny, G.M. (1997) Pheromone cCF10 and plasmid pCF10-encoded regulatory molecules act post-transcriptionally to activate expression of downstream conjugation functions. Mol Microbiol 24: 285-294.
7. Chen, E.Y., and Seeburg, P.H. (1985) Supercoiled sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4: 165-170.
8. Chow, J.W., Thai, L.A., Perri, M.B., Vazquez, J.A., Donabedian, S.M., Clewell, D.B., et al. (1993) Plasmid-associated hemolysin and aggregation substance production contributes to virulence in experimental enterococcal endocarditis. Antimicrob Agents Chemother 37: 2474-2477.
9. Clewell, D.B. (1998) Sex pheromone systems in enterococci. In Cell-Cell Signaling in Bacteria. Dunny, G.M., and Winans, S.C. (eds). Washington, DC: American Society for Microbiology Press, (in press).
10. Clewell, D.B. (1993a) Bacterial sex pheromone-induced plasmid transfer. Cell 73: 9-12.
11. Clewell, D.B. (1993b) Sex pheromones and the plasmid-encoded mating response in Enterococcus faecalis. In Bacterial Conjugation. Clewell, D.B. (ed.). New York: Plenum Press, pp. 349-367.
12. Clewell, D.B., Tomich, P.K., Gawron-Burke, M.C., Franke, A.E., Yagi, Y., and An, F.Y. (1982) Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J Bacteriol 152: 1220-1230.
13. Clewell, D.B., An, F.Y., White, B.A., and Gawron-Burke, C. (1985) Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918). J Bacteriol 162: 1212-1220.
14. Clewell, D.B., Pontius, L.T., An, F.Y., Ike, Y., Suzuki, A., and Nakayama, J. (1990) Nucleotide sequence of the sex pheromone inhibitor (iAD1) determinant of Enterococcus faecalis conjugative plasmid pAD1. Plasmid 24: 156-161.
15. Dunny, G.M., and Leonard, B.A.B. (1997) Cell-cell communication in Gram-positive bacteria. Annu Rev Microbiol 51: 527-564.
16. Dunny, G.M., Craig, R.A., Carron, R.L., and Clewell, D.B. (1979) Plasmid transfer in Streptococcus faecalis: production of multiple sex pheromones by recipients. Plasmid 2: 454-465.
17. Ehrenfeld, E.E., and Clewell, D.B. (1987) Transfer functions of the Streptococcus faecalis plasmid pAD1: organization of plasmid DNA encoding response to sex pheromone. J Bacteriol 169: 3473-3481.
18. Flannagan, S.E., and Clewell, D.B. (1991) Conjugative transfer of Tn916 in Enterococcus faecalis: trans activation of homologous transposons. J Bacteriol 173: 7136-7141.
19. Fujimoto, S., and Clewell, D.B. (1998) Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating, DNA-binding TraA protein. Proc Natl Acad Sci USA 95: 6430-6435.
20. Galli, D., Lottspeich, F., and Wirth, R. (1990) Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Mol Microbiol 4: 895-904.
21. Galli, D., Friesenegger, A., and Wirth, R. (1992) Transcriptional control of sex-pheromone-inducible genes on plasmid pAD1 of Enterococcus faecalis and sequence analysis of a third structural gene for (pPD1-encoded.) aggregation substance. Mol Microbiol 6: 1297-1308.
22. Ike, Y., and Clewell, D.B. (1984) Genetic analysis of the pAD1 pheromone response in Streptococcus faecalis, using transposon Tn917 as an insertional mutagen. J Bacteriol 158: 777-783.
23. Ike, Y., and Clewell, D.B. (1992) Evidence that the hemolysin/ bacteriocin phenotype of Enterococcus faecalis subsp. zymogenes can be determined by plasmids in different incompatibility groups as well as by the chromosome. J Bacteriol 174: 8172-8177.
24. Ike, Y., Craig, R.C., White, B.A., Yagi, Y., and Clewell, D.B. (1983) Modification of Streptococcus faecalis sex pheromones after acquisition of plasmid DNA. Proc Natl Acad Sci USA 80: 5369-5373.
25. Ike, Y., Hashimoto, H., and Clewell, D.B. (1984) Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice. Infect Immun 45: 528-530.
26. Ike, Y., Hashimoto, H., and Clewell, D.B. (1987) High incidence of hemolysin production by Enterococcus (Streptococcus) faecalis strains associated with parenteral infections. J Clin Microbiol 25: 1524-1528.
27. Jett, B.D., Jensen, H.G., Nordquist, R.E., and Gilmore, M.S. (1992) Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis. Infect Immun 60: 2445-2452.
28. Kao, S.-M., Olmsted, S.B., Viksnins, A.S., Gallo, J.C., and Dunny, G.M. (1991) Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis. J Bacteriol 173: 7650-7664.
29. Ladin, B.F., Accuroso, E.M., Mielenz, J.R., and Wilson, C.R. (1992) A rapid procedure for isolating RNA from small-scale Bacillus cultures. BioTechniques 12: 672-675.
30. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
31. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
32. Mori, M., Isogai, A., Sakagami, Y., Fujino, M., Kitada, C., Clewell, D.B., et al. (1986) Isolation and structure of Streptococcus faecalis sex pheromone inhibitor, iAD1, that is excreted by donor strains harboring plasmid pAD1. Agric Biol Chem 50: 539-541.
33. Mori, M., Sakagami, Y., Narita, M., Isogai, A., Fujino, M., Kitada, C., et al. (1984) Isolation and structure of the bacterial sex pheromone, cAD1, that induces plasmid transfer in Streptococcus faecalis. FEBS Lett 178: 97-100.
34. Muscholl, A., Galli, D., Wanner, G., and Wirth, R. (1993) Sex pheromone plasmid pAD1-encoded aggregation substance of Enterococcus faecalis is positively regulated in trans by traE1. Eur J Biochem 214: 333-338.
35. Pontius, L.T., and Clewell, D.B. (1992a) Regulation of the pAD1-encoded pheromone response in Enterococcus faecalis: nucleotide sequence analysis of traA. J Bacteriol 174: 1821-1827.
36. Pontius, L.T., and Clewell, D.B. (1992b) Conjugative transfer of Enterococcus faecalis plasmid pAD1: nucleotide sequence and transcriptional fusion analysis of a region involved in positive regulation. J Bacteriol 174: 3152-3160.
37. Tanimoto, K., and Clewell, D.B. (1993) Regulation of the pAD1-encoded sex pheromone response in Enterococcus faecalis: expression of the positive regulator TraE1. J Bacteriol 175: 1008-1018.
38. Tanimoto, K., An, F.Y., and Clewell, D.B. (1993) Characterization of the traC determinant of the Enterococcus faecalis hemolysin/bacteriocin plasmid pAD1. Binding of sex pheromone. J Bacteriol 175: 5260-5264.
39. Tao, L., and Ferretti, J.J. (1991) Cloning vectors with streptococcal resistance genes and an Escherichia coli origin of replication which can be used for cloning of streptococcal origins of replication or for gene inactivation in streptococci. In Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci. Dunny, G.M., Cleary, P.P., and McKay, L.L. (eds). Washington, DC: American Society for Microbiology Press, p. 305.
40. Tomich, P.K., An, F.Y., Damle, S.P., and Clewell, D.B. (1979) Plasmid related transmissibility an multiple drug resistance in Streptococcus faecalis subspecies zymogenes strain DS16. Antimicrob Agents Chemother 15: 828-830.
41. Tomich, P.K., An, F.Y., and Clewell, D.B. (1980) Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis. J Bacteriol 141: 1366-1374.
42. Weaver, K.E., and Clewell, D.B. (1988) Regulation of the pAD1 sex pheromone response in Enterococcus faecalis: construction and characterization of lacZ transcriptional fusions in a key control region of the plasmid. J Bacteriol 170: 4343-4352.
43. Weaver, K.E., and Clewell, D.B. (1989) Construction of Enterococcus faecalis pAD1 miniplasmids: identification of a minimal pheromone response regulatory region and evaluation of a novel pheromone-dependent growth inhibition. Plasmid 22: 106-119.
44. Weaver, K.E., and Clewell, D.B. (1990) Regulation of the pAD1 sex pheromone response in Enterococcus faecalis: effects of host strain and traA, traB, and C region mutants on expression of an E region pheromone-inducible lacZ fusion. J Bacteriol 172: 2633-2641.
45. Wirth, R. (1994) The sex pheromone system of Enterococcus faecalis. More than just a plasmid-collection mechanism? Eur J Biochem 222: 235-246.
46. Wirth, R., An, F.Y., and Clewell, D.B. (1986) Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector. J Bacteriol 165: 831-836.