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7 PFU of ultraviolet (UV)-inactivated HSV-1 (KOS). Supernatants harvested 48 hours later were tested for IL-10 and IL-12 p40 cytokine by sandwich enzyme-linked immunosorbent assay (ELISA) using OptEIA antibody sets (PharMingen, La Jolla, CA). IL-4 was measured after stimulation of draining lymph node cells by plate-bound antibody to CD3.
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0343900556
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data not shown
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0343028939
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note
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-/- mice contained liver-associated Listeria-infected cysts that were apparent 4 to 5 days after infection. Plates were incubated at 37°C, and the numbers of CFU were counted after 24 hours.
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37
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0343464754
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note
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8 CFU/ml) 96 hours before IFN-γ measurement by an OptEIA ELISA kit (PharMingen).
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note
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Addition of 5 mM GRGDS peptide but not GRADS peptide (29) to cultures of peritoneal macrophages (38) resulted in an 80% reduction of the IL-12 response after LPS stimulation.
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44
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0342594821
-
-
note
-
Single-letter abbreviations for the amino acid residues are as follows: A, Ala; D, Asp; E, Glu; G, Gly; L, Leu; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; and T, Thr.
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0029081709
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27
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Dephosphorylation of Eta-1 is as follows. Five milligrams of purified Eta-1 was dephosphorylated using 6 U (60 U/mg) of type-II potato acid phosphatase (Sigma) as described (39). Phosphorylation of recombinant Eta-1:glutathione S-transferase (GST)-Eta-1 (5 mg) was phosphorylated as described [S. Ashkar, J. L. Schaffer, E. Salih, L. C. Gerstenfeld, M. J. Glimcher, Ann. N.Y. Acad. Sci. 760, 296 (1995); (27)]. Phosphoamino acid analysis of the recovered protein revealed a phosphoserine content of 16 mol of phosphate per 1 mol of protein and 0.8 mol of phosphothreonine per 1 mol of protein.
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0343028932
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note
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-/- mice were coinjected with this inoculum of PVP and 10 μg of Eta-1 and purified as described below. After 5 days, mice were killed, tissue was extracted for histologic analysis, and local lymph nodes were obtained for cytokine expression. Samples were fixed in 10% buffered formalin and embedded in paraffin; 4- to 5-μm serial sections were stained with hematoxylin and eosin, and images were captured with a Sony DXC-970MD video camera and Optima 5.2 Histomorphometric analysis software.
-
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-
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60
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0027321589
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-
39
-
2-terminal and internal peptide analysis). Mass spectroscopic analysis revealed a peak centered around a mass of 35,400 daltons that was highly phosphorylated (11 mol of phosphate per 1 mol of protein), O-glycosylated but not N-glycosylated, and without measurable sulfate.
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3 was from PharMingen [J. F. Schultz and D. R. Armant, J. Biol. Chem. 270, 11522 (1995)], and antibody to CD44, KM81 (ATCC), was used to block the interaction between CD44 and Eta-1 (16).
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0342594819
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note
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2-terminal sequence QETLPSN (29)] was active and predicted to terminate at the thrombin cleavage site. This 10-kD fragment contained ∼5 mol of phosphate per 1 mol of peptide at seven potential phosphorylation sites.
-
-
-
-
63
-
-
0343900543
-
-
note
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-/- mice; B. J. Rollins for L. monocytogenes strain 1778; S. Hikita and D. Block for technical assistance; and A. Angel, K. MacKay, and L. Lagasse for assistance in the preparation of the manuscript.
-
-
-
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