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Volumn 287, Issue 5454, 2000, Pages 860-864

Eta-1 (osteopontin): An early component of type-1 (cell-mediated) immunity

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 10; INTERLEUKIN 12; OSTEOPONTIN;

EID: 0034603119     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5454.860     Document Type: Article
Times cited : (1027)

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    • Dephosphorylation of Eta-1 is as follows. Five milligrams of purified Eta-1 was dephosphorylated using 6 U (60 U/mg) of type-II potato acid phosphatase (Sigma) as described (39). Phosphorylation of recombinant Eta-1:glutathione S-transferase (GST)-Eta-1 (5 mg) was phosphorylated as described [S. Ashkar, J. L. Schaffer, E. Salih, L. C. Gerstenfeld, M. J. Glimcher, Ann. N.Y. Acad. Sci. 760, 296 (1995); (27)]. Phosphoamino acid analysis of the recovered protein revealed a phosphoserine content of 16 mol of phosphate per 1 mol of protein and 0.8 mol of phosphothreonine per 1 mol of protein.
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    • -/- mice; B. J. Rollins for L. monocytogenes strain 1778; S. Hikita and D. Block for technical assistance; and A. Angel, K. MacKay, and L. Lagasse for assistance in the preparation of the manuscript.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.