메뉴 건너뛰기




Volumn 279, Issue 5355, 1998, Pages 1344-1347

Molecular mimicry by herpes simplex virus-type 1: Autoimmune disease after viral infection

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN ANTIBODY COMPLEX; ARTICLE; AUTOIMMUNE DISEASE; CELL CLONING; HERPES SIMPLEX VIRUS; PRIORITY JOURNAL; TARGET ORGAN; VIRUS INFECTION;

EID: 0032570826     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5355.1344     Document Type: Article
Times cited : (447)

References (34)
  • 2
    • 0028791239 scopus 로고
    • G. Dahlquist et al., Diabetologia 38, 1371 (1995); P. B. Challoner et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7440 (1995); S. Vento et al., Lancet 346, 608 (1995).
    • (1995) Diabetologia , vol.38 , pp. 1371
    • Dahlquist, G.1
  • 3
    • 0029142819 scopus 로고
    • G. Dahlquist et al., Diabetologia 38, 1371 (1995); P. B. Challoner et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7440 (1995); S. Vento et al., Lancet 346, 608 (1995).
    • (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 7440
    • Challoner, P.B.1
  • 4
    • 0029129699 scopus 로고
    • G. Dahlquist et al., Diabetologia 38, 1371 (1995); P. B. Challoner et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7440 (1995); S. Vento et al., Lancet 346, 608 (1995).
    • (1995) Lancet , vol.346 , pp. 608
    • Vento, S.1
  • 7
    • 0023651203 scopus 로고
    • M. B. A. Oldstone, Cell 50, 819 (1987); M. G. von Herrath and M. B. A. Oldstone, Curr. Opin. Immunol. 8, 878 (1996).
    • (1987) Cell , vol.50 , pp. 819
    • Oldstone, M.B.A.1
  • 10
    • 0030925578 scopus 로고    scopus 로고
    • K. W. Wucherpfennig and J. L. Strominger, Cell 80, 695 (1995); B. Hemmer et al., J. Exp. Med. 185, 1651 (1997).
    • (1997) J. Exp. Med. , vol.185 , pp. 1651
    • Hemmer, B.1
  • 12
    • 0025754853 scopus 로고    scopus 로고
    • M. B. A. Oldstone, M. Nerenberg, P. Southern, J. Price, H. Lewicki, Cell 65, 319 (1991); P. Ohashi et al., ibid., p. 305.
    • Cell , pp. 305
    • Ohashi, P.1
  • 14
    • 0029151916 scopus 로고
    • A. C. Avery et al., Nature 376, 431 (1995).
    • (1995) Nature , vol.376 , pp. 431
    • Avery, A.C.1
  • 17
    • 0023899276 scopus 로고    scopus 로고
    • D. J. McGeoch et al., J. Gen. Virol. 69, 1531 (1997); J. Hay and W. T. Ruyechan, Curr. Topics Microbiol. Immunol. 179, 1 (1992); A. H. Patel and J. B. Maclean, Virology 206, 465 (1995).
    • (1997) J. Gen. Virol. , vol.69 , pp. 1531
    • McGeoch, D.J.1
  • 18
    • 0026755475 scopus 로고
    • D. J. McGeoch et al., J. Gen. Virol. 69, 1531 (1997); J. Hay and W. T. Ruyechan, Curr. Topics Microbiol. Immunol. 179, 1 (1992); A. H. Patel and J. B. Maclean, Virology 206, 465 (1995).
    • (1992) Curr. Topics Microbiol. Immunol. , vol.179 , pp. 1
    • Hay, J.1    Ruyechan, W.T.2
  • 19
    • 0028891403 scopus 로고
    • D. J. McGeoch et al., J. Gen. Virol. 69, 1531 (1997); J. Hay and W. T. Ruyechan, Curr. Topics Microbiol. Immunol. 179, 1 (1992); A. H. Patel and J. B. Maclean, Virology 206, 465 (1995).
    • (1995) Virology , vol.206 , pp. 465
    • Patel, A.H.1    Maclean, J.B.2
  • 20
  • 21
    • 0023737057 scopus 로고
    • M. B. Raizman and C. S. Foster, Curr. Eye Res. 7, 823 (1988); C. S. Foster et al., Ocular Immunol. Today (1990), p. 111.
    • (1990) Ocular Immunol. Today , pp. 111
    • Foster, C.S.1
  • 22
    • 0019504620 scopus 로고
    • 5 cells per 60-mm dish) were incubated for 24 hours after seeding and then transfected with 12 μg of DNA (including 0.5 μg of infectious KOS DNA, 5 μg of mutant plasmid DNA, and 6.5 μg of carrier DNA). Five days later, when generalized cytopathic effects were apparent, cultures were harvested and then frozen and thawed three times. The cell suspension was sonicated briefly, diluted 1:10 in medium, passed through a 0.22-μm filter, plated on monolayers of G33 cells-BHK21 cells expressing the UL6 protein [A. H. Patel et al., Virology 217, 111 (1996)] - and overlaid with methylcellulose. After incubation at 37°C for 4 days, plates were stained for 24 hours with medium containing neutral red, individual plaques were picked, and isolates were screened for their ability to produce cytopathic effects in G33 cells but not in Vero cells. Isolates with this phenotype were plaque-purified three times.
    • (1981) J. Virol. , vol.38 , pp. 50
    • Goldin, A.L.1
  • 23
    • 0029862621 scopus 로고    scopus 로고
    • 5 cells per 60-mm dish) were incubated for 24 hours after seeding and then transfected with 12 μg of DNA (including 0.5 μg of infectious KOS DNA, 5 μg of mutant plasmid DNA, and 6.5 μg of carrier DNA). Five days later, when generalized cytopathic effects were apparent, cultures were harvested and then frozen and thawed three times. The cell suspension was sonicated briefly, diluted 1:10 in medium, passed through a 0.22-μm filter, plated on monolayers of G33 cells-BHK21 cells expressing the UL6 protein [A. H. Patel et al., Virology 217, 111 (1996)] - and overlaid with methylcellulose. After incubation at 37°C for 4 days, plates were stained for 24 hours with medium containing neutral red, individual plaques were picked, and isolates were screened for their ability to produce cytopathic effects in G33 cells but not in Vero cells. Isolates with this phenotype were plaque-purified three times.
    • (1996) Virology , vol.217 , pp. 111
    • Patel, A.H.1
  • 24
    • 6844257561 scopus 로고    scopus 로고
    • note
    • m mutant viruses at a multiplicity of infection of 10 PFU per cell. Uninfected and infected cells were harvested 18 to 20 hours after infection by scraping into the medium and were centrifugea at 1500 revolutions per minute and 4°C for 10 min. The cell pellet was resuspended in 5 ml of phosphate-buffered saline (PBS), repelleted, and resuspended again in 1 ml of PBS. The cell suspension was stored at -70°C overnight, thawed at 37°C, and sonicated for 1 min. Samples were pelleted at 4°C for 10 min, and the supernatant fluid was divided into aliquots and stored at -70°C. Protein concentrations were determined by measuring absorbance at 280 nm and using bovine serum albumin as a standard.
  • 31
    • 0026533363 scopus 로고
    • The antigen used for in vitro stimulation was prepared from extracts of Vero cells infected with HSV-1 (KOS) (0.01 PFU per cell) and harvested 3 days after infection [W. Cai and P. A. Scharfer, J. Virol. 66, 2904 (1992)]. The virus suspension was inactivated by UV light (254 nm) for 20 min at a distance of 5 cm [L. Morrison and D. Knipe, J. Virol. 68, 689 (1994)]. Control antigen was prepared in the same way from extracts of uninfected cells.
    • (1992) J. Virol. , vol.66 , pp. 2904
    • Cai, W.1    Scharfer, P.A.2
  • 32
    • 0028047306 scopus 로고
    • The antigen used for in vitro stimulation was prepared from extracts of Vero cells infected with HSV-1 (KOS) (0.01 PFU per cell) and harvested 3 days after infection [W. Cai and P. A. Scharfer, J. Virol. 66, 2904 (1992)]. The virus suspension was inactivated by UV light (254 nm) for 20 min at a distance of 5 cm [L. Morrison and D. Knipe, J. Virol. 68, 689 (1994)]. Control antigen was prepared in the same way from extracts of uninfected cells.
    • (1994) J. Virol. , vol.68 , pp. 689
    • Morrison, L.1    Knipe, D.2
  • 34
    • 6844253879 scopus 로고    scopus 로고
    • note
    • We would like to thank D. Knipe for providing pSG10, A. H. Patel for G33 cells, J. Glorioso for KO82 mutant virus, and A. Angel for assistance in preparation of the manuscript. Supported by NIH grant AI 37562 to H.C.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.