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Volumn 288, Issue 5471, 2000, Pages 1647-1650

Essential role for cholesterol in entry of mycobacteria into macrophages

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL;

EID: 0034595877     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5471.1647     Document Type: Article
Times cited : (498)

References (31)
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    • J774 and bone marrow-derived macrophages were depleted of cholesterol as described [M. Simons et al., Proc. Natl. Acad. Sci. U.S.A. 95, 6460 (1998)], with the following modifications: J774 cells were first cultured (at 37°C) for 60 hours in lovastatin (4 μM, Calbiochem) and mevalonate (250 μM. Sigma), followed by a 45-min treatment at 37°C by methyl-β-cyclodextrin (10 mM, Sigma) in serum-free Dulbecco's modified essential medium (DMEM) in the continuous presence of lovastatin and mevalonate. Bone marrow-derived macrophages were depleted for 180 hours in lovastatin and mevalonate, and residual cholesterol was extracted for 75 min with 20 mM methyl-β-cyctodextrin.
    • (1998) Proc. Natl. Acad. Sci. U.S.A. , vol.95 , pp. 6460
    • Simons, M.1
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    • D. B. Young, Nature 393, 515 (1998).
    • (1998) Nature , vol.393 , pp. 515
    • Young, D.B.1
  • 26
    • 0342540479 scopus 로고    scopus 로고
    • note
    • 35S]methionine/cysteine (Promix, Amersham) for 12 hours at 37°C. M. bovis BCG harboring phsp60-gfp (8) was kindly provided by V. Deretic. M. tuberculosis strain H37Rv was kindly donated by B. Heym. Mycobacteria were propagated in 7H9 mycobacterial medium (Difco) supplemented with 10% OADC Middlebrook supplement (Difco).
  • 27
    • 0342974764 scopus 로고    scopus 로고
    • note
    • Bone marrow-derived macrophages were generated by incubating bone marrow cells from C57BL/6 mice for 7 days in DMEM containing 10% heat-inactivated fetal calf serum, 5% inactivated horse serum, penicillin and streptomycin, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, and 30% L-929-conditioned medium.
  • 28
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    • note
    • 600 = 2) for 1 hour at 37°C and washed twice before use.
  • 31
    • 0343410081 scopus 로고    scopus 로고
    • note
    • We thank V. Deretic, A. Dijkstra, S. Falkow, R. Valdivia, M. Shaw, and J. Thole for strains and reagents; M. Dessing, S. Meyer, and A. Traunecker for assistance with (video) microscopy; M. Adler for allowing access to P3 facilities; and T. Harder, M. Kopf, and W. Philipp for comments on the manuscript. The Basel Institute for Immunology was founded and is supported by Hoffmann-La Roche Ltd., Basel, Switzerland.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.