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Bone marrow-derived macrophages were generated by incubating bone marrow cells from C57BL/6 mice for 7 days in DMEM containing 10% heat-inactivated fetal calf serum, 5% inactivated horse serum, penicillin and streptomycin, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, and 30% L-929-conditioned medium.
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0343410082
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600 = 2) for 1 hour at 37°C and washed twice before use.
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We thank V. Deretic, A. Dijkstra, S. Falkow, R. Valdivia, M. Shaw, and J. Thole for strains and reagents; M. Dessing, S. Meyer, and A. Traunecker for assistance with (video) microscopy; M. Adler for allowing access to P3 facilities; and T. Harder, M. Kopf, and W. Philipp for comments on the manuscript. The Basel Institute for Immunology was founded and is supported by Hoffmann-La Roche Ltd., Basel, Switzerland.
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