Lack of a role for iron in the Lyme disease pathogen

Science

Volumn 288, Issue 5471, 2000, Pages 1651-1653

  Posey, James E ^a   Gherardini, Frank C ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IRON; METALLOPROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIUM CULTURE; BORRELIA BURGDORFERI; CULTURE MEDIUM; LYME DISEASE; NONHUMAN; PRIORITY JOURNAL;

BACTERIAL PROTEINS; BORRELIA BURGDORFERI GROUP; CALCIUM; CARBONYL CYANIDE M-CHLOROPHENYL HYDRAZONE; CHELATING AGENTS; CULTURE MEDIA; GENE EXPRESSION; GENOME, BACTERIAL; IRON; IRON CHELATING AGENTS; MAGNESIUM; MANGANESE; POLYSTYRENES; POLYVINYLS; UNCOUPLING AGENTS; ZINC;

ANIMALIA; BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI;

EID: 0034595617     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5471.1651     Document Type: Article

References (22)

1. S. M. Payne, Trends Microbiol. 1, 66 (1993).
2. K. G. Wooldridge and P. H. Williams, FEMS Microbiol. Rev. 12, 325 (1993).
3. J. J. Bullen, Rev. Infect. Dis. 3, 1127 (1981).
4. E. D. Weinberg, Microbiol. Rev. 42, 45 (1978).
5. R. L. Jurado, Clin. Infect. Dis. 25, 888 (1997).
6. H. A. Bledsoe et al., J. Bacteriol. 176, 7447 (1994).
7. C. M. Fraser et al., Nature 390, 580 (1997).
8. C. A. Whitehouse, L. R. Williams, F. E. Austin, Infect. Immun. 65, 4865 (1997).
9. H. Graeff-Wohlleben, S. Killat, A. Banemann, N. Guiso, R. Gross, J. Bacteriol. 179, 21941 (1997).
10. A. Amano et al., J. Bacteriol. 172, 1457 (1990).
11. M. E. Martin et al., J. Biol. Chem. 261, 9361 (1986).
12. L. Chen, L. P. James, J. D. Helman, J. Bacteriol. 175, 5428 (1993).
13. N. Bsat, A. Herbig, L. Casillas-Martinez, P. Setlow, J. D. Helman, Mol. Microbiol. 29, 189 (1998).
14. A. G. Barbour, Yale J. Biol. Med. 57, 521 (1984).
15. SF-E medium consisted of serum-free BSK-II (16) with the following modifications. The N-acetylglucosamine and pyruvate were increased to 0.64 and 1.28 g/liter, respectively. The bovine serum albumin was increased from 4 to 7% (w/v), and the medium was supplemented with 3-phosphoglyceric acid (0.48 g/liter), 0.8% (v/v) glycerol, and 2% (v/v) Excyte.
16. F. Archibald, FEMS Microbiol. Lett. 19, 29 (1983).
17. J. Ma et al., J. Bacteriol. 181, 3730 (1999).
18. S. Archibald and M. Duang, J. Bacteriol. 158, 1 (1984).
19. C. M. Fraser et al., Science 281, 375 (1998).
20. R. Himmelreich et al., Nucleic Acids Res. 24, 4420 (1996).
21. M. A. Markwell, S. M. Haas, L. L. Bieber, N. E. Tolbert, Anal. Biochem. 87, 206 (1978).
22. We thank T. Hoover and D. Krause for critical reading of the manuscript. Supported by National Institute of Allergy and Infectious Diseases grants AI33501 and AI144911.