Cloning and functional analysis of chicory root fructan 1-exohydrolase I (1-FEH I): A vacuolar enzyme derived from a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzyme

Plant Journal

Volumn 24, Issue 4, 2000, Pages 447-456

  Van Ende, Wim Den ^a   Michiels, An ^a   De Roover, Joke ^a   Verhaert, Peter ^b   Van Laere, André ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b Zoological Institute   (Belgium)

Author keywords

1 FEH; Cichorium intybus; Fructan breakdown; Inulin; MALDI TOF

Indexed keywords

ANALYTIC METHOD; BETA FRUCTOFURANOSIDASE; CDNA CLONE; ENZYME LOCALIZATION; ENZYME PURIFICATION; FRUCTAN 1 EXOHYDROLASE I; HETEROLOGOUS EXPRESSION; MESSENGER RNA; MOLECULAR EVOLUTION; PHYLOGENY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BETA-FRUCTOFURANOSIDASE; BLOTTING, NORTHERN; CELL WALL; CHICORY; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FRUCTANS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLYCOSIDE HYDROLASES; HEXOSYLTRANSFERASES; HYDROLASES; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RNA, PLANT; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SOLANUM TUBEROSUM; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TISSUE DISTRIBUTION; TRYPSIN; VACUOLES;

CICHORIUM INTYBUS; EMBRYOPHYTA; SOLANUM TUBEROSUM;

EID: 0034548367     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2000.00890.x     Document Type: Article

References (39)

1. Intracellular trafficking of secretory proteins
2. Extracellular localization of chitinase in cucumber
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. Regulation of expression of the fructan hydrolase gene of streptococcus mutans GS-5 by induction and carbon catabolite repression
5. Purification and properties of an inulinase from chicory roots (Cichorium intybus L.)
6. Purification and properties of a second fructan exohydrolase from the roots of Cichorium intybus L.
7. Drought induces fructan synthesis and 1-SST (sucrose: sucrose 1-fructosyl transferase) in roots and leaves of chicory seedlings (Cichorium intybus L.)
8. The mechanism of fructosan metabolism in higher plants as exemplified in Helianthus tuberosus
9. Localisation of fructan metabolism in the vacuoles isolated from protoplasts of Jerusalem artichoke
10. Current and potential food and non-food applications of fructans
11. Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers
12. Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots
13. Evolutionary origins and natural functions of fructans. A climatological, biogeographic and mechanistic appraisal
14. Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes β-2,6 fructans
15. Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages
16. Production of modified polymeric carbohydrates
17. Plant fructans stabilize phosphatidylcholine liposomes during freeze-drying
18. Invertase activity associated with the walls of Solanum tuberosum tubers
19. Molecular cloning and sequence analysis of an endo-inulinase gene from Arthrobacter sp.
20. Apoplastic sugars, fructans, fructan exohydrolase, and invertase in winter oat: Responses to second-phase cold hardening
21. Error-tolerant identification of peptides in sequence databases by peptide sequence tags
22. Seasonal variation of fructan-β-fructosidase (FEH) activity and characterisation of a β-(2,1)-linkage specific FEH
23. Hydrolysis of fructan in grasses: A β-(2,6)-linkage specific fructan-β-fructosidase from stubble of Lolium perenne
24. Improved performance of transgenic fructan-accumulating tobacco under drought stress
25. Molecular characterization of the gene for carrot cell-wall β-fructosidase
26. The bifidogenic nature of chicory inulin and its hydrolysis products
27. Both developmental and metabolic signals activate the promotor of a class I patatin gene
28. Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning
29. Inhibition of dietary inulin and oligofructose on the growth of transplantable mouse tumor
30. Fructan synthesizing and degrading activities in chicory roots (Cichorium intybus L.) during growth, storage and forcing
31. The metabolism of fructans in roots of Cichorium intybus L. during growth, storage and forcing
32. In vitro synthesis of fructofuranosyl-only oligosaccharides from inulin and fructose by purified chicory root fructan: Fructan fructosyl transferase
33. Purification and characterization of 1-SST, the key enzyme initiating fructan biosynthesis in young chicory roots (Cichorium intybus L.)
34. Purification and characterization of fructan: Fructan fructosyl transferase from chicory roots (Cichorium intybus L.)
35. Cloning, developmental and tissue-specific expression of 1-SST (sucrose: sucrose 1-fructosyl transferase) from Taraxacum officinale. Fructan localization in roots
36. Fructan: More than a reserve carbohydrate?
37. Fructan metabolism, enzymology and compartmentation
38. Fructans - An extension of sucrose by sucrose