Purification and characterization of 1-SST, the key enzyme initiating fructan biosynthesis in young chicory roots (Cichorium intybus)

Physiologia Plantarum

Volumn 98, Issue 3, 1996, Pages 455-466

  Van Den Ende, Wim ^a   Van Wonterghem, Dominik ^a   Dewil, Erna ^b   Verhaert, Peter ^c   De Loof, Arnold ^c   Van Laere, André ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b Laboratory of Physiology and Immunology of Domestic Animals   (Belgium)

^c Zoological Institute   (Belgium)

Author keywords

1 kestose; 1 SST; Chicory; Cichorium intybus; Fructan; Inulin; Invertase; Sucrose

Indexed keywords

CICHORIUM INTYBUS;

EID: 0030513914     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.1996.980304.x     Document Type: Article

References (55)

1. Arai, M., Mori, H. & Imaseki, H. 1992. Cloning and sequence of cDNAs for an intracellular acid invertase from etiolated hypocotyls of mung bean and expression of the gene during growth of seedlings. - Plant Cell Physiol. 33: 245-252.
2. Aslanidis, C., Schmid, K. & Schmitt, R. 1989. Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli. - J. Bacteriol. 171: 6753-6763.
3. Blake, M. S., Johnston, K. H., Russel-Jones, G. J. & Gotschlich, E. C. 1984. A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blots. - Anal. Biochem. 136: 175-179.
4. Bracho, G. E. & Whitaker, J. R. 1990. Purification and partial characterization of potato (Solanum tuberosum) invertase and its endogenous proteinaceous inhibitor. - Plant Physiol. 92: 386-394.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254.
6. Cairns, A. J. 1993. Evidence for the de novo synthesis of fructan by enzymes from higher plants: A reappraisal of the 1-SST/ 1-FFT model. - New Phytol. 123: 15-24.
7. _ & Ashton, J. E. 1991. The interpretation of in vitro measurements of fructosyl transferase activity: An analysis of patterns of fructosyl transfer by fungal invertase. - New Phytol. 118: 23-34.
8. Chambert, R. & Petit-Glatron, M.-F. 1991. Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis. - Biochem. J. 279: 35-41.
9. Chandorkar, K. R. & Collins, F. W. 1974. Enzymological aspects of de novo synthesis of fructooligosaccharides in leaf disks of certain Asteraceae. IV. The activity of sucrose:sucrose 1-fructosyl transferase. - Can. J. Bot. 52: 1369-1377.
10. Chatterton, N. J., Harrison, P. A., Thornley, W. R. & Benett, J. H. 1988. Characterization of sucrose:sucrose fructosyl transferase from crested wheatgrass. - New Phytol. 109: 29-33.
11. Darwen, C. W. E. & John, P. 1989. Localisation of the enzymes of fructan metabolism in vacuoles isolated by a mechanical method from tubers of Jerusalem artichoke (Helianthus tuberosus L.). - Plant Physiol 89: 658-663.
12. Dedonder, R. 1972. Role and mechanisms of transglycosylation reactions. - In Biochemistry of the Glycosidic Linkage (R. Piras and H. G. Pontis, eds), pp. 21-78. Academic Press, New York, NY.
13. Duchateau, N., Bortlik, K., Simmen, U., Wiemken, A. & Bancal, P. 1995. Sucrose:fructan 6-fructosyltransferase, a key enzyme for diverting carbon from sucrose to fructan in barley leaves. - Plant Physiol. 107: 1249-1255.
14. Edelman, J. & Jefford, T. G. 1968. The mechanism of fructosan metabolism in higher plants as exemplified in Helianthus tuberosus. - New Phytol. 67: 517-531.
15. Elliott, K. J., Butler, W. O., Dickinson, C. D., Konno, Y., Vedvick, T. S., Fitzmaurice, L. & Mirkov, T. E. 1993. Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening. - Plant Mol. Biol. 21: 515-524.
16. Fouet, A., Klier, A. & Rapoport, G. 1986. Nucleotide sequence of the sucrase gene from Bacillus subtilis. - Gene 45: 221-225.
17. Gupta, A. K., Mamta & Bhatia, I. S. 1985. Glucofructosan metabolism in Cichorium intybus roots. - Phytochemistry 24: 1423-1428.
18. Hendry, G. 1993. Evolutionary origins and natural functions of fructans. A climatological, biogeographic and mechanistic appraisal. - New Phytol. 123: 3-14.
19. Henry, R. J. & Darbyshire, B. 1980. Sucrose:sucrose fructosyl transferase and fructan:fructan fructosyl transferase from Allium cepa. - Phytochemistry 19: 1017-1020.
20. Hidaka, H., Hirayama, M. & Yamada, K. 1991. Fructooligosaccharides. Enzymatic preparation and biofunctions. - J. Carbohydr. Chem. 10: 509-522.
21. Hohmann, S. & Gozalbo, D. 1988. Structural analysis of the 5′ regions of yeast SUC genes revealed analogous palindromes in SUC, MAL and GAL. - Mol. Gen. Genet. 211: 446-454.
22. Jahreis, K. & Lengeler, J. W. 1993. Molecular analysis of two ScrR repressors and of a ScrR-FruR hybrid repressor for sucrose and D-fructose specific regulons from enteric bacteria. - Mol. Microbiol. 9: 195-209.
23. Klein, R. D., Poorman, R. A., Favreau, M. A., Shea, M. H., Hatzenbuhler, N. T. & Nulf, S. C. 1989. Cloning and sequencing of the gene encoding invertase from the yeast Schwanniomyces occidentalis. - Curr. Genet. 16: 145-152.
24. Koops, A. J. & Jonker, H. H. 1994. Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus 'Colombia'. I. Fructan:fructan fructosyl transferase. - J. Exp. Bot: 45: 1623-1631.
25. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 227: 680-685.
26. Laloux, O., Cassart, J.-P., Van Beeuwen, J., Delcour, J. & Vandenhaute, J. 1991. Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424. - FEBS Lett. 289: 64-68.
27. Lüscher, M. & Nelson, C. J. 1995. Fructosyltransferase activities in the leaf growth zone of tall fescue. - Plant Physiol. 107: 1419-1425.
28. Martin, I., Debarbouille, M., Ferrari, E., Klier, A. & Rapoport, G. 1987. Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase. - Mol. Gen. Genet. 208: 177-184.
29. Meier, H. & Reid, J. S. G. 1982. Reserve polysaccharides other than starch in higher plants. - In Encyclopedia of Plant Physiology, New Series, Vol. 13A, Plant Carbohydrates (F. A. Loewus and W. Tanner, eds), pp. 418-471. Springer-Verlag, Berlin. ISBN 3-540-11060-7.
30. Merrill, C. R., Goldman, D., Sedman, S. A. & Ebert, M. H. 1982. Ultrasensitive stain for proteins in polyacrylamide gels shows variation in cerobrospinal fluid proteins. - Science 211: 1437.
31. Pilon-Smits, E. A. H., Ebskamp, M. J. M., Jeuken, M. J. W., Weisbeek, P. J. & Smeekens, S. C. M. 1995. Improved performance of transgenic fructan-accumulating tobacco under drought stress. - Plant Physiol. 107: 125-130.
32. Plowman, K. M. 1972. Enzyme Kinetics. - McGraw-Hill, New York, NY.
33. F-β-D-fructosyl-transferase from tubers of Helianthus tuberosus L. - Agric. Biol. Chem. 54: 2429-2431.
34. Ramloch-Lorenz, K., Knudsen, S. & Sturm, A. 1993. Molecular characterization of the gene for carrot cell wall β-fructosidase. - Plant J. 43: 545-554.
35. Sato, Y., Poy, F., Jacobson, G. R. & Kuramitsu, H. K. 1989. Sequence analysis of the Streptococcus mutans scrB gene. - J. Bacteriol. 171: 263-271.
36. Scholle, R. R. 1989. Nucleotide sequence and analysis of the Vibrio alginolyticus sucrase gene (scrB). - Gene 80: 49-56.
37. Shiomi, N. & Izawa, M. 1980. Purification and characterisation of sucrose:sucrose 1-fructosyl transferase from the roots of asparagus (Asparagus officinalis L.). - Agric. Biol. Chem. 44: 603-614.
38. F-β-D fructosyl transferase in onion seeds. - Phytochemistry 24: 695-698.
39. _ , Onodera, S., Chatterton, N. J. & Harrison, P. A. 1991. Separation of fructofuranosyl-only oligosaccharide isomers by anion-exchange chromatography. - Agric. Biol. Chem. 55: 1427-1428.
40. Singh, R. & Bhatia, I. S. 1971. Isolation and characterization of fructosyltransferase from chicory roots. - Phytochemistry 10: 495-502.
41. Sprenger, N., Bortlik, K., Brandt, A., Boller, T. & Wiemken, A. 1995. Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley. - Proc. Natl. Acad. Sci. USA 92: 11652-11656.
42. Straathof, A. J. J., Kieboom, A. P. G. & Van Bekkum, H. 1986. Invertase-catalysed fructosyl transfer in concentrated solutions of sucrose. - Carbohydr. Res. 146: 154-159.
43. Unger, C., Hardegger, M., Lienhardt, S. & Sturm, A. 1994. cDNA cloning of carrot (Daucus carota) soluble acid β-fructofuranosidases and comparison with the cell wall isoenzyme. - Plant Physiol. 104: 1351-1357.
44. Van den Ende, W. & Van Laere, A. 1993. Purification and properties of an invertase with sucrose:sucrose fructosyltransferase (SST) activity from the roots of Cichorium intybus L. - New Phytol. 123: 31-37.
45. _ & Van Laere, A. 1995. Purification and properties of a neutral invertase from the roots of Cichorium intybus. - Physiol. Plant. 93: 241-248.
46. _ & Van Laere, A. 1996a. Fructan synthesizing and degrading activities in chicory roots (Cichorium intybus L.) during field-growth, storage and forcing. - J. Plant Physiol. (In press).
47. _ & Van Laere, A. 1996b. De novo synthesis of fructans from sucrose in vitro by a combination of two purified enzymes (sucrose:sucrose 1-fructosyl transferase and fructan:fructan 1-fructosyl transferase) from chicory roots (Cichorium intybus L.). - Planta (In press).
48. _ , De Roover, J. & Van Laere, A. 1996a. In vitro synthesis of fructofuranosyl-only oligosacchandes from inulin and fructose by purified chicory root fructan:fructan fructosyl transferase. - Physiol. Plant. 97: 346-352.
49. _ , Mintiens, A., Speleers, H., Onuoha, A. A. & Van Laere, A. 1996b. The metabolism of fructans in roots of Cichorium intybus L. during growth, storage and forcing. - New Phytol. 132: 555-563.
50. _ , Van Wonterghem, D., Verhaert, P., Dewil, E. & Van Laere, A. 1996c. Purification and characterization of fructan:fructan fructosyl transferase from chicory roots (Cichorium intybus L.). - Planta 199 (In press).
51. Wagner, W. & Wiemken, A. 1987. Enzymology of fructan synthesis in grasses. - Plant Physiol. 85: 706-710.
52. Weil, M., Krausgrill, S., Schuster, A. & Rausch, T. 1994. A 17-kDa Nicotiana tabacum cell-wall peptide acts as an in vitro inhibitor of the cell-wall isoform of acid invertase. - Planta 193: 438-445.
53. Wiemken, A., Frehner, M., Keller, F. & Wagner, W. 1986. Fructan metabolism, enzymology and compartmentation. - Curr. Top. Plant Biochem. Physiol. 5: 17-37.
54. Xu, J., Pemberton, G. H., Almira, E. C., McCarty, D. R. & Koch, K. E. 1995. The ivr gene for invertase in maize. - Plant Physiol. 108: 1293-1294.
55. Yanase, H., Fukushi, H., Ueda, N., Maeda, Y., Toyoda, A. & Tonomura, K. 1991. Cloning, sequencing and characterization of the intracellular invertase gene from Zymomonas mobilis. - Agric. Biol. Chem. 55: 1383-1390.