Identification of a cryptic N-terminal signal in Saccharomyces cerevisiae peroxisomal citrate synthase that functions in both peroxisomal and mitochondrial targeting

Journal of Biochemistry

Volumn 128, Issue 6, 2000, Pages 1059-1072

  Lee, Jeong Goo ^a   Cho, Seong Pil ^a,d   Lee, Heon Sik ^a   Lee, Cheong Ho ^b   Bae, Kyung Sook ^c   Maeng, Pil Jae ^a  

^a Chungnam National University   (South Korea)

^b Korea Ginseng Corporation   (South Korea)

^c Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^d Mogam Biotechnology Research Institute   (South Korea)

Author keywords

Citrate synthase; Mitochondria; Peroxisomes; Saccharomyces cerevisiae; Targeting signal

Indexed keywords

AMINO ACID; CHIMERIC PROTEIN; CITRATE SYNTHASE; GLUTAMIC ACID; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MITOCHONDRIAL ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; AUXOTROPHY; FUSION GENE; GENE; GENE CIT1; GENE CIT2; GENE DISRUPTION; GENE MUTATION; MITOCHONDRION; NONHUMAN; PEROXISOME; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN TARGETING; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CITRATE (SI)-SYNTHASE; DNA PRIMERS; DNA, FUNGAL; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PEROXISOMES; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, NUCLEIC ACID;

SACCHAROMYCES CEREVISIAE;

EID: 0034533501     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022834     Document Type: Article

References (46)

1. Targeting of proteins into mitochondria
2. Protein sorting in mitochondria
3. A conserved tripeptide sorts proteins to peroxisomes
4. Urate oxidase is imported into peroxisomes recognizing the C-terminal SKL motif of proteins
5. Carboxyl-terminal consensus Ser-Lys-Leu-related tripeptide of peroxisomal proteins functions in vitro as a minimal peroxisome-targeting signal
6. Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo
7. The peroxisomal targeting signal of 3-oxoacyl-CoA thiolase from Saccharomyces cerevisiae
8. Thiolase mRNA translated in vitro yields a peptide with a putative N-terminal presequence
9. Occurrence of 2 closely related but differentially regulated gene
10. A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase
11. Glyoxysomal malate dehydrogenase from watermelon is synthesized with an amino-terminal transit peptide
12. Peroxisomal amine oxidase of Hansenula polymorpha does not require its SRL-containing C-terminal sequence for targeting
13. The N-terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal
14. Acyl-CoA oxidase contains two targeting sequences each of which can mediate protein import into peroxisomes
15. Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae
16. Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method
17. Saccharomyces cerevisiae contains two functional citrate synthase genes
18. Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal
19. Mitochondrial and nonmitochondrial citrate synthase in Saccharomyces cerevisiae are encoded by distinct homologous genes
20. The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase
21. Purification and characterization of nonmitochondrial citrate synthase from Saccharomyces cerevisiae
22. Organelle targeting of citrate synthases in Saccharomyces cerevisiae
23. Improved green fluorescence [letter]
24. Transformation of intact yeast cells treated with alkali cations
25. Yeast/E. coli shuttle vectors with multiple unique restriction sites
26. Molecular Cloning: A Laboratory Manual
27. RTG1 and RTG2: Two yeast genes required for a novel path of communication from mitochondria to the nucleus
28. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
29. Truncated green fluorescent protein mutants and their expression in Aplysia neurons
30. Sequence of the Saccharomyces cerevisiae CTA1 gene and amino acid sequence of catalase A derived from it
31. Citrate synthase
32. Cytochrome c oxidase from baker's yeast: I. Isolation and Properties
33. Tissue fractionation studies, 17. Intracellular distribution of monoamine oxidase, aspartate amino transferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat liver tissue
34. Alternative topogenic signals in peroxisomal citrate synthase of Saccharomyces cerevisiae
35. The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals
36. 2-terminal peptide
37. Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor
38. Mutational analysis of the N-terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha
39. Aberrant subcellular localization of peroxisomal 3-ketoacyl-CoA thiolase in the Zellweger syndrome and rhizomelic chondrodysplasia punctata
40. Acyl-CoA oxidase, peroxisomal thiolase and dihydroxyacetone phosphate acyltransferase: Aberrant subcellular localization in Zellweger syndrome
41. PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae
42. The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL
43. Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: Evidence that PTS1 protein import is mediated by a cycling receptor
44. The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene
45. Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor
46. Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways