Sites of limited proteolysis in the pyruvate decarboxylase component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and their role in catalysis

European Journal of Biochemistry

Volumn 267, Issue 24, 2000, Pages 7158-7169

  Chauhan, Hitesh J ^a   Domingo, Gonzalo J ^a   Jung, Hyo Il ^a   Perham, Richard N ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Enzyme catalysis; Limited proteolysis; Multienzyme complex; Pyruvate decarboxylase; Pyruvate dehydrogenase

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; DIHYDROLIPOAMIDE ACETYLTRANSFERASE; PYRUVATE DECARBOXYLASE; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID;

ACETYLATION; ARTICLE; CATALYSIS; CONTROLLED STUDY; DECARBOXYLATION; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME DEGRADATION; GEOBACILLUS STEAROTHERMOPHILUS; IN VITRO STUDY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REDUCTION;

AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; CATALYSIS; CHYMOTRYPSIN; HYDROLYSIS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PYRUVATE DECARBOXYLASE; PYRUVATE DEHYDROGENASE COMPLEX; SURFACE PLASMON RESONANCE; TRYPSIN;

EID: 0034533110     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01820.x     Document Type: Article

References (49)

1. Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: A paradigm in the design of a multifunctional enzyme
2. Swinging arms and swinging domains in multifunctional enzymes: Catalytic machines for multistep reactions
3. The pyruvate dehydrogenase multienzyme complex from Gram-negative bacteria
4. The high-resolution structure of the peripheral subunit-binding domain of dihydrolipoamide acetyltransferase from the pyruvate dehydrogenase complex of Bacillus stearothermophilus
5. Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes
6. Structure-function relationships in dihydrolipoamide acyltransferases
7. 2) in vitro
8. Active-site modification of mammalian pyruvate dehydrogenase by pyridoxal 5'-phosphate
9. A common structural motif in thiamin pyrophosphate-binding enzymes
10. Cloning and expression in Escherichia coli of mature E1β subunit of bovine mitochondrial branched-chain alpha-keto acid dehydrogenase complex - Mapping of the E1 beta binding region on E2
11. Interaction of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: Stoichiometry and specificity in self assembly
12. Competitive interaction Of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: Kinetic analysis using surface plasmon resonance detection
13. Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
14. Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: Mechanism for rate enhancement in a multimeric structure
15. Rapid intersite transfer of acetyl groups and movement of pyruvate dehydrogenase components in the kidney pyruvate dehydrogenase complex
16. Kinetics and specificity of reductive acylation of wild-type and mutated lipoyl domains of 2-oxo acid dehydrogenase complexes from Azotobacter vinelandii
17. Studies on the nature and reactions of protein-bound lipoic acid
18. Kinetics and specificity of reductive acylation of lipoyl domains from 2-oxo acid dehydrogenase multienzyme complexes
19. Reconstitution of pyruvate dehydrogenase multienzyme complexes based on chimeric core structures from Azotobacter vinelandii and Escherichia coli
20. Recognition of a surface loop of the lipoyl domain underlies substrate channelling in the pyruvate dehydrogenase multienzyme complex
21. 2 relaxation experiments: The lipoyl domain and E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus
22. Crystal structure of 2-oxoisovalerate dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes
23. Crystal structure of human branched-chain α-keto acid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease
24. Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from Escherichia coli
25. Limited proteolysis of the pyruvate dehydrogenase multienzyme complex of Escherichia coli
26. Limited proteolysis and proton NMR spectroscopy of the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli
27. Repeating functional domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli
28. Inner core and domain structure of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus
29. Limited proteolysis and proton NMR spectroscopy of Bacillus stearothermophilus pyruvate dehydrogenase multi-enzyme complex
30. Bovine kidney pyruvate dehydrogenase complex. Limited proteolysis and molecular structure of the lipoate acetyltransferase component
31. Limited proteolysis of 2-oxoglutarate dehydrogenase multienzyme complex from bovine kidney
32. Subunit interactions in the mammalian α-ketoglutarate dehydrogenase complex. Evidence for direct association of the α-ketoglutarate dehydrogenase and dihydrolipoamide dehydrogenase components
33. Expression and characterization of the homodimeric E1 component of the Azotobacter vinelandii pyruvate dehydrogenase complex
34. Pyruvate dehydrogenase from Azotobacter vinelandii - Properties of the N-terminally truncated enzyme
35. Expression of genes encoding the E2 and E3 components of the Bacillus stearothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interaction in assembly in vitro
36. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
37. Expression in Escherichia coli of a sub-gene encoding the lipoyl and peripheral subunit-binding domains of the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex of Bacillus stearothermophilus
38. Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. Molecular cloning and characterization of the lplA gene and gene product
39. Structural determinants of post-translational modification and catalytic specificity for the lipoyl domains of the pyruvate dehydrogenase multienzyme complex of Escherichia coli
40. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
41. Study of the kinetic mechanism of pyruvate-2,6-dichlorophenolindophenol reductase activity of muscle pyruvate dehydrogenase
42. 1H-n.m.r. spectroscopy of the pyruvate dehydrogenase complex of Bacillus stearothermophilus
43. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
44. The structural basis of substrate activation in yeast pyruvate decarboxylase. A crystallographic and kinetic study
45. Determination of the number of active-centers in the pyruvate dehydrogenase component of the pyruvate dehydrogenase complex from pigeon breast muscle
46. Correlation between sites of limited proteolysis and segmental mobility in thermolysin
47. Probing the conformational state of apomyoglobin by limited proteolysis
48. Proteinase-catalyzed activation of porcine heart muscle pyruvate dehydrogenase and identification of its cleavage site
49. Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli