Choline import into chloroplasts limits glycine betaine synthesis in tobacco: Analysis of plants engineered with a chloroplastic or a cytosolic pathway

Metabolic Engineering

Volumn 2, Issue 4, 2000, Pages 300-311

  Nuccio, Michael L ^a   McNeil, Scott D ^a   Ziemak, Michael J ^a   Hanson, Andrew D ^a   Jain, Ravinder K ^b   Selvaraj, Gopalan ^b  

^a UNIVERSITY OF FLORIDA   (United States)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Choline; Choline monooxygenase; Choline oxidase; Glycine betaine; Nicotiana tabacum; Pathway modeling

Indexed keywords

NICOTIANA TABACUM; SPINACIA OLERACEA; TABACUM;

EID: 0034503411     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2000.0158     Document Type: Article

References (52)

1. Enhanced tolerance to light stress of transgenic Arabidopsis plants that express the codA gene for a bacterial choline oxidase
2. Binary Agrobacterium vectors for plant transformation
3. Transport and phosphorylation of choline in higher plant cells: Phosphorus-31 nuclear magentic resonance studies
4. Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): Structural similarity to mammalian ALDHs and a plant BADH
5. Plant productivity and the environment
6. Evidence for a ferredoxin-dependent choline monooxygenase from spinach chloroplast stroma
7. Assay, purification, and partial characterization of choline monooxygenase from spinach
8. Import and routing of nucleus-encoded chloroplast proteins
9. Improved high-level constitutive foreign gene expression in plants using an AMV RNA4 untranslated leader sequence
10. Modified binary plant transformation vectors with the wild type gene encoding NPTII
11. Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress
12. Plant viral leaders influence expression of a reporter gene in tobacco
13. Translational control of cellular and viral mRNAs
14. Choline kinase activities in choline-sensitive and choline-resistant tobacco cells or calluses grown on high choline concentrations. Effects on phosphatidylcholine synthesis
15. Control of mRNA stability in higher plants
16. An expression cassette for targeting foreign proteins into choloroplasts
17. Equilibrium constants under physiological conditions for the reactions of choline kinase and the hydrolysis of phosphorylcholine to choline and inorganic phosphate
18. 14C tracer evidence for synthesis of choline and betaine via phosphoryl base intermediates in salinized sugarbeet leaves
19. Improving stress tolerance in plants by gene transfer
20. A simple and general method for transferring genes into plants
21. Solubilization, partial purification, and reconstitution of the glycolate/glycerate transporter from chloroplast inner envelope membranes
22. Genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: Metabolic limitations
23. Molecular genetic improvement of salt tolerance in plants
24. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments
25. The promoter of TL-DNA gene 5 controls the tissue specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector
26. Optimizing expression of transgenes with an emphasis on posttranscriptional events
27. Rapid and efficient site-directed mutagenesis without phenotypic selection
28. DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli
29. Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli
30. Transient expression analysis in plants using firefly luciferase reporter gene
31. Radiotracer and computer modeling evidence that phosphobase methylation is the main route of choline synthesis in tobacco
32. Metabolic modeling identifies key constraints on an engineered glycine betaine synthesis pathway in tobacco
33. The complete nucleotide sequence of the small-subunit ribosomal RNA coding region for the cycad Zamia pumila: Phylogenetic implications
34. The endogenous choline supply limits glycine betaine synthesis in transgenic tobacco expressing choline monooxygenase
35. Metabolic engineering of plants for osmotic stress resistance
36. AtS1 and AtS3, two novel embryo-specific genes in Arabidopsis thaliana
37. Metabolic engineering of glycine betaine synthesis: Plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance
38. Choline monooxygenase, an unusual iron-sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants: Prosthetic group characterization and cDNA cloning
39. Quaternary ammonium and tertiary sulfonium compounds in higher plants
40. Determination of betaine by fast atom bombardment mass spectrometry. Identification of glycine betaine deficient genotypes of maize
41. Choline oxidase, a catabolic enzyme in Arthrobacter pascens, facilitates adapation to osmotic stress in Escherichia coli
42. Osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth
43. Choline synthesis in spinach in relation to salt stress
44. Choline kinase and phophorylcholine phosphatase in plants
45. Transgenically expressed betaine aldehyde dehydrogenase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and ω-aminoaldehydes
46. Choline oxidation by intact spinach chloroplasts
47. Betaine aldehyde dehydrogenase from spinach leaves: Purification, in vitro translation of the mRNA, and regulation by salinity
48. Enzymes of choline synthesis in spinach. Response of phospho-base N-methytransferase activities to light and salinity
49. Subcellular volumes and metabolite concentrations in spinach leaves
50. Arabidopsis thaliana small subunit leader and transit peptide enhance the expression of Bacillus thuringiensis proteins in transgenic plants
51. Compatible and counteracting solutes