Closed chamber PCR chips for DNA amplification

Engineering Science and Education Journal

Volumn 9, Issue 6, 2000, Pages 259-264

  Schabmueller, C G J ^a   Lee, M A ^b   Evans, A G R ^a   Brunnschweiler, A ^a   Ensell, G J ^a   Leslie, D L ^b  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b DEFENCE SCIENCE AND TECHNOLOGY LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DIAGNOSIS; DNA; MICROMACHINING; REACTION KINETICS;

DNA REPLICATION; OPTICAL DETECTION; POLYMERASE CHAIN REACTION (PCR);

GENETIC ENGINEERING;

EID: 0034499694     PISSN: 09637346     EISSN: None     Source Type: Journal    
DOI: 10.1049/esej:20000605     Document Type: Article

References (17)

1. ERLICH, H. A. (Ed.): 'PCR technology: principles and applications for DNA amplification' (Stockton Press, 1989)
2. NORTHRUP, M. A., CHING, M. T., WHITE, R. M., and WATSON, R. T.: 'DNA amplification with a microfabricated reaction chamber'. Proc. Transducers '93, Chicago, USA, 1993, pp.924-926
3. BELGRADER, P., et al.: 'Multi-chamber, real time DNA analysis in the field using microfabricated silicon chambers'. Proc. ASME 1998 Congress, 15th-20th November 1998, Anaheim, CA, DSC-Vol. 66: 'Micro-electro-mechanical systems: MEMS', pp.279-283
4. DANIEL, J. H., IQBAL, S., MILLINGTON, R. B., MOORE, D. F., LOWE, C. R., LESLIE, D. L., LEE, M. A., and PEARCE, M. J.: 'Silicon microchambers for DNA amplification', Sens. Actuators A, 1998, 71, (1-2), pp.81-88
5. KOPP, M., MELLO, A., MANZ, A.: 'Chemical amplification: continuous-flow PCR on a chip', Science, 1998, 280, pp.1046-1048
6. KOEHLER, J. M., DILLNER, U., MOKANSKY, A., POSER, S., and SCHULZ, T.: 'Micro channel reactors for fast thermocycling'. Proc. Process Miniaturization: 2nd Int. Conf. on Microreaction Technology, 1998, New Orleans, USA, pp.241-247
7. HIGUCHI, R., DOLLINGER, G., WALSH, P., and GRIFFITH, R.: 'Simultaneous amplification and detection of specific DNA sequences', Bio/Technology, 1992, 10, pp.413-417
8. WITTWER, C. T., HERRMANN, M. G., MOSS, A. A., and RASMUSSEN, R. P.: 'Continuous fluorescence monitoring of rapid cycle DNA amplification', BioTechniques, 1997, 22, pp.130-138
9. MORRISON, T. B., WEISS, J. J., and WITTWER, C. T.: 'Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification', BioTechniques, 1998, 24, pp.954-962
10. SELVIN, P. R.: 'Fluorescence resonance energy transfer', Methods in Enzymology, 1995, 246, pp.300-335
11. GELMINI, S., ORLANDO, C., SESTINI, R., VONA, G., PINZANI, P., RUOCCO, L., and MARIO, P.: 'Quantitative polymerase chain reaction based homogeneous assay with fluorogenic probes to measure c-erB-2 oncogene amplification', Clinical Chemistry, 1997, 43, (5), pp.752-758
12. TYAGI, S., and KRAMER, F. R.: 'Molecular beacons: probes that fluoresce upon hybridization', Nature Biotechnology, 1996, 14, pp.303-308
13. NAZARENKO, I., BAHTNAGAR, S. K., and HOHMAN, R. J.: 'A closed tube format for amplification and detection of DNA based on energy transfer', Nucleic Acid Research, 1997, 25, pp.2516-2521
14. WHITCOMBE, D., THEAKER, J., GUY, S. P., BROWN, T., and LITTLE, S.: 'Detection of PCR products using self quenching probing amplicons and fluorescence', Nature Biotechnology, 1999, 17, pp.804-807
15. LEE, M. A., BRIGHTWELL, G., LESLIE, D., BIRD, H., and HAMILTON, A.: 'Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR', J. Appl. Microbiology, 1999, 87, pp.218-223
16. MAYER, G. K., OFFEREINS, H. L., SANDMAIER, H., and KUEHL, K.: 'Fabrication of non-underetched convex corners in anisotropic etching of (100)-silicon in aqueous KOH with respect to novel micromechanic elements', J. Electrochem. Soc., 1990, 137, (12), pp.3947-3951
17. SANDMAIER, H., OFFEREINS, H. L., KUEHL, K., and LANG, W.: 'Corner compensation techniques in anisotropic etching of (100)-silicon using aqueous KOH'. Proc. Transducers '91, June 1991, San Francisco, CA, pp.456-459 (IEEE Press)