Fluorescent detection techniques for real-time multiplex strand specific detection of Bacillus anthracis using rapid PCR

Journal of Applied Microbiology

Volumn 87, Issue 2, 1999, Pages 218-223

  Lee, M A ^a   Brightwell, G ^a   Leslie, D ^a   Bird, H ^a   Hamilton, A ^a  

^a DEFENCE SCIENCE AND TECHNOLOGY LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; BACTERIA; BACTERIOLOGY; FINANCE; FLUORESCENCE;

AMPLICONS; B.ANTHRACIS; BACILLUS ANTHRACIS; FLUORESCENT DETECTION; FLUORESCENT METHODS; INTERNAL CONTROLS; LIGHTCYCLER; PCR ASSAY; REAL- TIME; SPECIFIC DETECTION;

POLYMERASE CHAIN REACTION;

ACCURACY; ARTICLE; BACILLUS ANTHRACIS; BACTERIUM DETECTION; DEVICE; FLUORESCENCE; NONHUMAN; POLYMERASE CHAIN REACTION;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0032820667     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.1999.00908.x     Document Type: Article

References (24)

1. Bassam, B.J., Allen, T., Flood, S. et al. (1996). Nucleic acid sequence detection systems: Revolutionary automation for monitoring and reporting PCR producers. Australasian Biotechnology 6(5), 285-284.
2. Cardullo, R.A., Agrawal, S., Flores, C. et al. (1988) Detection of nucleic acid hybridisation by nonradiative fluorescence resonance energy Transfer. Proc. Natl. Acad. Sci. USA 85, 8790-8794.
3. Daniel, J.H., Iqbal, S., Millington, R.B. et al. (1998). Silicon microchambers for DNA amplification. Sensors and Actuators. 71, 81-88.
4. Findlay, J.B., Atwood, S.M., Bergmeyer, L. et al. (1993). Automated closed vessel system for in vitro diagnostics based on the polymerase chain reaction. Clin Chem. 39, 1927-1933.
5. Gustafson, C.E., Alm, R.A. and Trust, T.J. (1993) Effect of heat denaturation of target DNA on the PCR amplification. Gene 123, 241-224.
6. Holland, P.M., Abramson, R.D., Watson, R. et al. (1991) Detection of specific polymerase chain reaction product by utilising the 5'-3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci., USA 88, 7276-72780.
7. Kellogg, D.E., Rybalkin, L, Chen, S. et al. (1994). TaqStart Antibody: 'Hot start' PCR facilitated by a neutralising monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16, 1134-1137.
8. Kramer, R.K. and Tyagi, S. (1996) Molecular beacons: probes that fluoresce upon hybridisation. Nature Biotech. 14, 303-308.
9. Lee, L.G., Connell, C.R. and Bloch, W. (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucl. Acids. Res. 21, 3761-3766.
10. Livak, K.J., Flood, S.J.A., Marmaro, J. et al. (1995). Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridisation. PCR Meth. Appl. 4, 357-362.
11. Nazarenko, L.A., Bhatnagar, S.K. and Hohman, R.J. (1997) A closed tube format for amplification and detection of DNA based on energy transfer. Nucl. Acids. Res. 25, 2516-2521.
12. Northrup, M.A., Ching, M.T., White, R.M. et al. (1993) DNA amplification in a microfabricated reaction chamber. Proc. 7th International Conference on Solid-State Sensors and Actuators (Transducers '93). Yokohama, Japan, 7 10 June, 924-926.
13. Northrup, M.A., Gonzalez, C., Hadley, D. et al. (1995) A MEMS based miniature DNA analysis system. Proc 8th International Conference on Solid State Sensors and Actuators (Transducers '95). Stockholm, Sweden, 25-29 June, pp. 764-767.
14. Odelberg, S.L. and White, R. (1993) A method for accurate amplification of polymorphic CA-repeat sequences. PCR Meth. Appl. 3, 7-12.
15. Poser, S., Schulz, T., Dillner, U. et al. (1997) Siebert chip elements for fast thermal cycling, Sensors Actuators A 62, 672-675.
16. Sharkey, D.J., Scalice, E.R., Christy, K.G. Jr. et al. (1994). Antibodies as thermolabile switches: high temperature triggering for the polymerase chain reaction. Biol Technology. 12, 506-509.
17. Swerdlow, H., Dew-Jager, K. and Gesteland, R.F. (1993) Rapid cycle sequencing in an air thermal cycler. Biotechniques. 15, 512-519.
18. Tan, S.T. and Weis, J.H. (1992) Development of a sensitive reverse transcriptase PCR assay, RT-PCR, utilising rapid cycle times. PCR Meth. Appl 2, 137-143.
19. Wittwer, C.T. and Garling, D.J. (1991) Rapid Cycle DNA amplification: time and temperature optimisation. Biotechniques 10, 76-83.
20. Wittwer, C.T., Herrman, A.A., Rasmussen, R.P. et al. (1997a) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques. 22, 130-138.
21. Wittwer, C.T., Reed, G.B. and Ririe, K.M. (1994) Rapid cycle DNA amplification, p. 174-181. In: The Polymerase Chain Reaction.(eds Mullis, K.B., Ferre, F. and Gibbs, R.A.) Birkhäuser, Boston.
22. Wittwer, C.T., Ririe, K.M., Andrew, R.V. et al. (1997a) The Light-Cycler™: a microVol. multisample fluorimeter with rapid temperature control. Biotechniques. 22, 176-181.
23. Wittwer, C.T., Ririe, K.M. and Rasmussen, R.P. (1997b) Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 245, 154-160.
24. Woolley, A.T., Hadley, D., Landre, P. et al. (1996) Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. Anal Chem. 68, 4081-4086.