Evolution of binding affinity in a WW domain probed by phage display

Protein Science

Volumn 9, Issue 12, 2000, Pages 2366-2376

  Dalby, Paul A ^a   Hoess, Ronald H ^b   DeGrado, William F ^c  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b DUPONT COMPANY   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Cooperativity; Peptide binding; Phage display; Protein scaffold; Specificity; Stability; WW domain

Indexed keywords

PROTEINASE K;

ARTICLE; BACTERIOPHAGE; BETA SHEET; BINDING AFFINITY; CIRCULAR DICHROISM; CONSENSUS SEQUENCE; DISSOCIATION CONSTANT; EVOLUTION; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CARRIER PROTEINS; CONSENSUS SEQUENCE; DIRECTED MOLECULAR EVOLUTION; ENDOPEPTIDASE K; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE LIBRARY; PHOSPHOPROTEINS; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; TEMPERATURE;

EID: 0034493023     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1017/S096183680000239X     Document Type: Article

References (35)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: The proline glycine and methionine-rich motif
3. A novel pro-Arg motif recognized by WW domains
4. The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules
5. The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila enabled
6. A single point mutation in a group I WW domain shifts its specificity to that of group II WW domains
7. Two binding orientations for peptides to the Src SH3 domain: Development of a general model for SH3-ligand interactions
8. Core-directed protein design. II. Rescue of a multiply mutated and destabilized variant of ubiquitin
9. Beta-hairpins in proteins revisited: Lessons for de novo design
10. Selection for improved protein stability by phage display
11. WW: An isolated three-stranded antiparallel beta-sheet domain that unfolds and refolds reversibly; evidence for a structured hydrophobic cluster in urea and GdnHCl and a disordered thermal unfolded state
12. Selectively-infective phage (SIP): A mechanistic dissection of a novel in vivo selection for protein-ligand interactions
13. Proteolytic selection for protein folding using filamentous bacteriophages
14. Solvent-exposed tryptophans probe the dynamics at protein surfaces
15. Structure and function of a new phosphopeptide-binding domain containing the FHA2 of Rad53
16. Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains
17. Using molecular repertoires to identify high-affinity peptide ligands of the WW domain of human and mouse YAP
18. Function of WW domains as phosphoserine- or phosphothreonine-binding modules
19. Structural analysis of WW domains and design of a W-W prototype
20. Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide
21. Physical and functional interactions between the transactivation domain of the hematopoietic transcription factor NF-E2 and WW domains
22. Exploiting the basis of proline recognition by SH3 and WW domains: Design of N-substituted inhibitors
23. Identification of novel peptide antagonists for GPIIb/IIIa from a conformationally constrained phage peptide library
24. How to measure and predict the molar absorption coefficient of a protein
25. Signaling through scaffold, anchoring, and adaptor proteins
26. Pi-turns in proteins and peptides: Classification, conformation, occurrence, hydration, and sequence
27. Structural and functional analysis of the mitotic rotamase Pin1 suggests substrate recognition is phosphorylation dependent
28. Amino acid preferences for specific locations at the ends of alpha helices
29. Filamentous phage assembly: Morphogenetically defective mutants that do not kill the host
30. From Src Homology domains to other signaling modules: Proposal of the "protein recognition code"
31. Structural basis for phosphoserine-proline recognition by group IV WW domains
32. Characterization of the interaction of natural proline-rich peptides with five different SH3 domains
33. In vitro evolution of thermodynamically stable turns
34. Recognition and specificity in protein tyrosine kinase-mediated signaling