Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p

Genes to Cells

Volumn 5, Issue 12, 2000, Pages 1039-1048

  Saito Nakano, Y ^a   Nakano, A ^a  

^a RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COAT PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; MEMBRANE PROTEIN; MUTANT PROTEIN;

ARTICLE; CELL GROWTH; CELL MUTANT; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE DELETION; GENE FUNCTION; GENE OVEREXPRESSION; GENE REPRESSION; LETHALITY; MEMBRANE BINDING; MICROSOME; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PURIFICATION;

ENZYME ACTIVATION; FUNGAL PROTEINS; GTP PHOSPHOHYDROLASES; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; GUANOSINE TRIPHOSPHATE; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MONOMERIC GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0034487342     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2000.00391.x     Document Type: Article

References (36)

1. Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum
2. COPII: A membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum
3. SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER
4. Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast
5. Yeast SEC16 gene encodes a multidomain vesicle coat protein that interacts with Sec23p
6. SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation
7. COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p
8. Genes that allow yeast cells to grow in the absence of the HDEL receptor
9. Sec23p and a novel 105-kDa protein as a multimeric complex to romote vesicle budding and protein transport from the endoplasmic reticulum
10. Distinct sets of SEC genes govern transport vasicle formation and fusion early in the secretory pathway
11. COPII-cargo interactions direct protein sorting into ER-derived transport vesicles
12. Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro
13. Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments
14. Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae
15. COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes
16. The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec12 mutant: Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum
17. Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation
18. A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast
19. A novel GTP-binding protein, Sar1, is involved in transport from the endoplasmic reticulum to the Golgi apparatus
20. Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum
21. Inhibition of GTP hydrolysis by Sar1p causes accumulation of vesicles that are a functional intermediate of the ER-to-Golgi transport in yeast
22. Reconstitution of GTP-binding Sar1 protein function in ER to Golgi transport
23. RHO gene products, putative small GTP-binding proteins, are important for activation of the CAl1/CDC43 gene products, a protein geranylgeranyltransferase in Saccharomyces cerevisiae
24. Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis and cell-free transport from the endoplasmic reticulum to the Golgi apparatus
25. Identification of SEC12, SED4, truncated SEC16 and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase
26. SEC31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum
27. Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain
28. Coat proteins and vesicle budding
29. COPII subunit interactions in the assembly of the vesicle coat
30. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
31. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs
32. A primer on vesicle budding
33. Isolation of a tobacco cDNA encoding Sar1 GTPase and analysis of its dominant mutations in vesicular traffic using a yeast complementation system
34. A dominant negative mutant of Sar1 GTPase inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus in tobacco and Arabidopsis cultured cells
35. Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum
36. Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum