The N-terminal region of cystatin A (stefin A) binds to papain subsequent to the two hairpin loops of the inhibitor. Demonstration of two-step binding by rapid-kinetic studies of cystatin A labeled at the N-terminus with a fluorescent reporter group

Protein Science

Volumn 9, Issue 11, 2000, Pages 2218-2224

  Estrada, Sergio ^a   Raub Segall, Elke ^a   Björk, Ingemar ^a   Olson, Steven T ^b  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Cystatin; Fluorescence labeling; N terminal region; Papain; Stefin; Stopped flow fluorescence; Two step binding

Indexed keywords

PAPAIN; STEFIN A;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

ANIMALS; CHICKENS; CYSTATINS; CYSTEINE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; HUMANS; KINETICS; PAPAIN; PROTEIN BINDING; SPECTROPHOTOMETRY;

EID: 0034486197     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.11.2218     Document Type: Article

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