Multiple lysine mutations in the C-terminal domain of p53 interfere with MDM2-dependent protein degradation and ubiquitination

Molecular and Cellular Biology

Volumn 20, Issue 24, 2000, Pages 9391-9398

  Nakamura, S ^a   Roth, J A ^a   Mukhopadhyay, T ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; COMPLEMENTARY DNA; LEPTOMYCIN B; LYSINE; MUTANT PROTEIN; PROTEIN MDM2; PROTEIN P53;

ACETYLATION; AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL SUBPOPULATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DNA BINDING; GENE MUTATION; HUMAN; HUMAN CELL; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN LOCALIZATION; TRANSACTIVATION;

ACETYLATION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CARCINOMA, NON-SMALL-CELL LUNG; CELL FRACTIONATION; ECDYSONE; FATTY ACIDS, UNSATURATED; GENE EXPRESSION REGULATION; GENES, P53; GENES, REPORTER; GTPASE-ACTIVATING PROTEINS; HUMANS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LUNG NEOPLASMS; NUCLEAR PROTEINS; PLASMIDS; POINT MUTATION; PRECIPITIN TESTS; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MDM2; TRANS-ACTIVATION (GENETICS); TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0034458966     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.24.9391-9398.2000     Document Type: Article

References (37)

1. The ubiquitin-proteasome pathway: On protein death and cell life
2. WAF1, a potential mediator of p53 tumor suppression
3. Nuclear export is required for degradation of endogenous p53 by MDM2 and human papillomavirus E6
4. A transcriptionally active DNA-binding site for human p53 protein complexes
5. Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain
6. Cell type-specific inhibition of p53-mediated apoptosis by mdm2
7. Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53
8. Association of p19(ARF) with Mdm2 inhibits ubiquitin ligase activity of Mdm2 for tumor suppressor p53
9. Regulation of the cryptic sequence-specific DNA-binding function of p53 by protein kinases
10. Regulation of the specific DNA binding function of p53
11. Activation of p53 sequence-specific DNA binding by short single strands of DNA requires the p53 C-terminus
12. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia
13. p53: Puzzle and paradigm
14. Regulation of p53 stability by Mdm2
15. Regulation of Mdm2-directed degradation by the C terminus of p53
16. Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein
17. Human TAFII31 protein is a transcriptional coactivator of the p53 protein
18. Oligomerization is required for p53 to be efficiently ubiquitinated by MDM2
19. In vivo ubiquitination and proteasome-mediated degradation of p53
20. Activation of p53 DNA binding activity by point mutation
21. Multisite phosphorylation and the integration of stress signals at p53
22. Tumor suppressor p53 is a direct transcriptional activator of human bax gene
23. The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation
24. A codon 248 p53 mutation retains tumor suppressor function as shown by enhancement of tumor growth by antisense p53
25. Oncoprotein MDM2 conceals the activation domain of tumour suppressor p53
26. Regulation of the p53 tumor suppressor protein
27. DNA damage activates p53 through a phosphorylation-acetylation cascade
28. Regulation of specific DNA binding by p53: Evidence for a role for O-glycosylation and charged residues at the carboxy-terminus
29. DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2
30. DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization
31. A leucine-rich nuclear export signal in the p53 tetramerization domain: Regulation of subcellular localization and p53 activity by NES masking
32. A C-terminal alpha-helix plus basic region motif is the major structural determinant of p53 tetramerization
33. Constitutive expression of the cyclin-dependent kinase inhibitor p21 is transcriptionally regulated by the tumor suppressor protein p53
34. 1160
35. Precise epitope mapping of the murine transformation associated protein, p53
36. The p53-mdm2 autoregulatory feedback loop
37. Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53