Sulfolobus shibatae CCA-adding enzyme forms a tetramer upon binding two tRNA molecules: A scrunching-shuttling model of CCA specificity

Journal of Molecular Biology

Volumn 304, Issue 3, 2000, Pages 483-492

  Li, Fang ^a   Wang, Jimin ^a   Steitz, Thomas A ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

Gel electrophoresis band mobility shift assay; Multi angle laser light scattering; RNA polymerase; Small angle X ray scattering

Indexed keywords

BACTERIAL ENZYME; NUCLEOTIDE; RNA POLYMERASE; TETRAMER; TRANSFER RNA;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME INDUCTION; ENZYME SPECIFICITY; ENZYME STABILITY; GEL ELECTROPHORESIS; LIGHT SCATTERING; MOLECULAR MODEL; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; RADIATION SCATTERING; SULFOLOBUS;

BACTERIA (MICROORGANISMS); SULFOLOBUS; SULFOLOBUS SHIBATAE;

EID: 0034405429     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4189     Document Type: Article

References (20)

1. Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae
2. Purification and properties of Escherichia coli CTP (ATP)-tRNA nucleotidyltransferase
3. Structural basis for initiation of transcription from an RNA polymerise-promoter complex
4. tRNA nucleotidyltransferase
5. Equilibrium and kinetics of 1ac repressor-operator interactions by polyacrylamide gel electrophoresis
6. Compromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases
7. La diffraction des rayons X aux tres peptits angles: Application a l'etude de phenomenes ultramicroscopiques
8. Crystal structure and evolution of a transfer RNA splicing enzyme
9. Dissection of the active site of rabbit liver tRNA nucleotidyltransferase. Specificity and properties of subsites for donor nucleotide triphosphates
10. Structure-specific tRNA-binding protein from the extreme thermophile Aquifex aeolicus
11. CCA addition by tRNA nucleotidyltransferase: Polymerization without translocation?
12. Insights into editing from an Ile-tRNA synthetase structure with tRNA(Ile) and mupirocin
13. DNA polymerases: Structural diversity and common mechanisms
14. Tyrosyl-tRNA synthetase acts as an asymmetric dimer in charging tRNA. A rationale for half-of-the-sites activity
15. Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interactions
16. Light scattering and the absolute characterization of macromolecules
17. CCA-adding enzymes and poly(A) polymerases are all members of the same nucleotidyltransferase superfamily: Characterization of the CCA-adding enzyme from the archaeal hyperthermophile Sulfolobus shibatae
18. The CCA-adding enzyme has a single active site
19. tRNA nucleotidyltransferase is not essential for Escherichia coli viability