SRP-dependent co-translational targeting and SecA-dependent translocation analyzed as individual steps in the export of a bacterial protein

EMBO Journal

Volumn 19, Issue 23, 2000, Pages 6419-6426

  Neumann Haefelin, Christoph ^a   Schäfer, Ute ^a   Müller, Matthias ^a   Koch, Hans Georg ^a  

^a Institut für Biochemie und Molekularbiologie   (Germany)

Author keywords

Membrane proteins; Protein targeting; SecA; Signal recognition particle (SRP); Trigger factor

Indexed keywords

BACTERIAL PROTEIN; CELL MEMBRANE PROTEIN; SIGNAL RECOGNITION PARTICLE;

ARTICLE; CELL MEMBRANE TRANSPORT; ESCHERICHIA COLI; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING;

ADENOSINE TRIPHOSPHATASES; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CARRIER PROTEINS; CELL MEMBRANE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE TRANSPORT PROTEINS; MODELS, BIOLOGICAL; MUTATION; PEPTIDYLPROLYL ISOMERASE; PERIPLASM; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; PROTON-TRANSLOCATING ATPASES; RECOMBINANT FUSION PROTEINS; RIBOSOMES; SIGNAL RECOGNITION PARTICLE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034387983     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.23.6419     Document Type: Article

References (32)

1. Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
2. Requirements for the translocation of elongation-arrested, ribosome-associated OmpA across the plasma membrane of Escherichia coli
3. The signal sequence moves through a ribosomal runnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation
4. Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore
5. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
6. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
7. A high yield modification of mutation by overlap extension using three primers
8. Biogenesis of the Gram-negative bacterial envelope
9. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
10. A signal recognition particle in Escherichia coli?
11. Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecA
12. The translocon: A dynamic gateway at the ER membrane
13. A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane
14. Translocation, folding and stability of the HflKC complex with signal anchor topogenic sequences
15. Dissecting the translocase and integrase functions of the Escherichia coli SecYEG translocon
16. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
17. The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle
18. Protein translocation: Tunnel vision
19. SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane
20. A site specific, membrane-dependent cleavage event defines the membrane binding domain of FtsY
21. Binding of signal recognition particle gives ribosome-nascent chain complexes a competitive advantage in endoplasmic reticulum membrane interaction
22. The structure of multiple polypeptide domains determines the signal recognition particle targeting requirement of Escherichia coli inner membrane proteins
23. Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA
24. SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle
25. Signal recognition particle-dependent targeting of ribosomes to the rough endoplasmic reticulum in the absence and presence of the nascent polypeptide associated complex
26. YidC mediates membrane protein insertion in bacteria
27. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein
28. Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions
29. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition panicle and Sec machinery
30. The E. Coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
31. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
32. The Escherichia coli SRP and SecB targeting pathways converge at the translocon