Coupling high enzyme activity and stability: A challenging target

Trends in Biotechnology

Volumn 18, Issue 10, 2000, Pages 401-402

  Carrea, Giacomo ^a   Colombo, Giorgio ^a  

^a CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; ENZYME; SUBTILISIN;

BACILLUS SUBTILIS; CATALYST; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME STABILITY; EVOLUTION; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE; TEMPERATURE; THERMOSTABILITY;

BACILLUS SUBTILIS; BIOTECHNOLOGY; CATALYSIS; ENZYME STABILITY; ENZYMES; MUTAGENESIS; SULFOLOBUS; TEMPERATURE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0034308293     PISSN: 01677799     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7799(00)01500-6     Document Type: Conference Paper

References (11)

1. Carrea G., Riva S. Properties and synthetic applications of enzymes in organic solvents. Angew. Chem. Int. Ed. Engl. 39:2000;2226-2254.
2. Hough D.W., Danson M.J. Extremozymes. Curr. Opin. Chem. Biol. 3:1999;39-46.
3. Zhang T., et al. Enhancement of protein stability by the combination of point mutations in T4 lysozyme is additive. Protein Eng. 8:1995;1017-1022.
4. Wrba A., et al. Extremely thermostable d-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima. Biochemistry. 29:1990;7584-7592.
5. Varley P.G., Pain R.H. Relation between stability, dynamics and enzyme activity in 3-phosphoglycerate kinases from yeast and Thermus thermophilus. J. Mol. Biol. 220:1991;531-538.
6. Knapp S., et al. Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 Å resolution. J. Mol. Biol. 267:1997;916-932.
7. Aguilar C.F., et al. Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability. J. Mol. Biol. 271:1997;789-802.
8. Zhao H., Arnold F.H. Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Eng. 12:1999;47-52.
9. Zhao H., et al. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat. Biotechnol. 16:1998;258-261.
10. Lazaridis T., et al. Dynamics and unfolding pathways of a hyperthermophilic and a mesophilic rubredoxin. Protein Sci. 6:1997;2589-2605.
11. Colombo G., Merz K.M. Stability and activity of mesophilic subtilisin E and its thermophilic homolog: insights from molecular dynamics simulations. J. Am. Chem. Soc. 121:1999;6895-6903.