Peptide electroextraction for direct coupling of in-gel digests with capillary LC-MS/MS for protein identification and sequencing

Analytical Chemistry

Volumn 72, Issue 17, 2000, Pages 4115-4121

  Timperman, Aaron T ^a   Aebersold, Ruedi ^b  

^a WEST VIRGINIA UNIVERSITY   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONATE DEHYDRATASE; GLYCOGEN PHOSPHORYLASE B;

AMINO ACID SEQUENCE; ARTICLE; CAPILLARY CHROMATOGRAPHY; EXTRACTION; INTERMETHOD COMPARISON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

EID: 0034282758     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac000305w     Document Type: Article

References (9)

1. Staudenmann, W.; Hatt, P. D.; Hoving, S.; Lehmann, A.; Kertesz, M.; James, P. Electrophoresis 1998, 19, 901-908.
2. Costello, C. E. Curr. Opin. Biotechnol. 1999, 10, 22-28.
3. Haynes, P. A.; Gygi, S. P.; Figeys, D.; Aebersold, R. Electrophoresis 1998, 19, 1862-1871.
4. Settlage, R. E.; Russo, P. S.; Shabanowitz, J.; Hunt, D. F. J. Microcolumn Sep. 1998, 10, 281-285.
5. Yates, J. R. J. Mass Spectrom. 1998, 33, 1-19.
6. Winston, R. L.; Fitzgerald, M. C. Mass Spectrom. Rev. 1997, 16, 165-179.
7. Yates, J. R. Electrophoresis 1998, 19, 893-900.
8. Fenyo, D.; Qin, J.; Chait, B. T. Electrophoresis 1998, 19, 998-1005.
9. Eng, J.; McCormack, A. L.; Yates, J. R. I. J. Am. Soc. Mass Spectrom. 1994, 5, 976-989.