Creating addressable aqueous microcompartments above solid supported phospholipid bilayers using lithographically patterned poly(dimethylsiloxane) molds

Analytical Chemistry

Volumn 72, Issue 11, 2000, Pages 2587-2589

  Yang, Tinglu ^a   Simanek, Eric E ^a   Cremer, Paul ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMETICONE; PHOSPHOLIPID;

AQUEOUS SOLUTION; ARTICLE; BLEACHING; FLUORESCENCE MICROSCOPY; LIGAND BINDING; PHOSPHOLIPID BILAYER; PHOSPHOLIPID MEMBRANE; PHOTOCHEMISTRY; RECEPTOR BINDING; SCREENING;

EID: 0034214155     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac000131i     Document Type: Article

References (19)

1. Tözeren, A.; Sung, K. P.; Sung, L. A.; Dustin, M. L.; Chan, P. Y.; Springer, T. A.; Chien, S. J. Cell Biol. 1992, 116, 997-1006.
2. Chan, P. Y.; Lawrence, M. B.; Ferguson, L. M.; Golan, D. E.; Springer, T. A. J. Cell Biol. 1991, 115, 245-255.
3. Sackmann, E. Science 1996, 277, 43-48.
4. Cremer, P. S.; Yang, T. J. Am. Chem. Soc. 1999, 121, 8130.
5. Xia, Y.; Whitesides, G. M. Angew. Chem., Int. Ed. 1998, 37, 550.
6. Groves, J. T.; Ulman, N.; Boxer, S. G. Science 1997, 275, 651.
7. Groves, J. T.; Ulman, N.; Cremer, P. S.; Boxer, S. G. Langmuir 1998, 14, 3347.
8. Kumar, A.; Whitesides, G. M. Appl. Phys. Lett. 1993, 63, 2002.
9. Delamarche, E.; Bernard, A.; Schmid, H.; Michel, B.; Biebuyck, H. Science 1997, 276, 779.
10. Brian, A.; McConnell, H. M. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 6159.
11. Axelrod, D.; Koppel, D. E.; Schlessinger, J.; Elson, E.; Webb, W. W. Biophys. J. 1976, 16, 1055.
12. Soumpasis, D. M. Biophys. J. 1983, 41, 97.
13. Duffy, D. C.; McDonald, J. C.; Schueller, O. J. A.; Whitesides, G. M. Anal. Chem. 1998, 70, 4974.
14. Calvert, T. L.; Leckband, D. Langmuir 1997, 13, 6737.
15. The final concentrations of these species are attenuated by a factor of ∼20 through dilution as the aqueous container volumes were roughly 150 nL.
16. Some streptavidin was observed to adsorb to the walls of the PDMS mold. This process could be suppressed by preadsorbing bovine serum albumin as suggested in ref 9.
17. Axelrod, D.; Burghardt, T. P.; Thompson, N. L. Annu. Rev. Biophys. Bioeng. 1984, 13, 247.
18. Mammen, M.; Choi, S. K.; Whitesides, G. M. Angew. Chem., Int. Ed. 1998, 37, 2754.
19. Kiessling, L. L.; Pohl, N. L. Chem. Biol. 1996, 3, 71.