A synthetic peptide based on a glycine-gated chloride channel induces a novel chloride conductance in isolated epithelial cells

Biochimica et Biophysica Acta - Biomembranes

Volumn 1466, Issue 1-2, 2000, Pages 47-60

  Mitchell, Kathy E ^a   Iwamoto, Takeo ^a   Tomich, John ^a   Freeman, Lisa C ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

Channel forming peptide; Chloride transport; Cystic fibrosis transmembrane conductance regulator; Epithelial cell

Indexed keywords

CHLORIDE CHANNEL; GLYCINE; SYNTHETIC PEPTIDE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CHLORIDE CONDUCTANCE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; EPITHELIUM CELL; IN VITRO STUDY; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CELL SEPARATION; CHLORIDE CHANNELS; CHLORIDES; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DOGS; ELECTRIC CONDUCTIVITY; ELECTROPHYSIOLOGY; ELECTROSTATICS; EPITHELIAL CELLS; GLYCINE; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDES; SOLUTIONS;

EID: 0034213807     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(00)00170-X     Document Type: Article

References (40)

1. Riordan J.R., Rommens J.M., Kerem B., Alon N., Rozmahel R., Grzelczak Z., Zielenski J., Lok S., Plavsic N., Chou J.L. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science. 245:1989;1066-1073.
2. Berger H.A., Anderson M.P., Gregory R.J., Thompson S., Howard P.W., Maurer R.A., Mulligan R., Smith A.E., Welsh M.J. Identification and regulation of the cystic fibrosis transmembrane conductance regulator-generated chloride channel. J. Clin. Invest. 88:1991;1422-1431.
3. Denning G.M., Ostedgaard L.S., Cheng S.H., Smith A.E., Welsh M.J. Localization of cystic fibrosis transmembrane conductance regulator in chloride secretory epithelia. J. Clin. Invest. 89:1992;339-349.
4. Riordan J.R. The cystic fibrosis transmembrane conductance regulator. Annu. Rev. Physiol. 55:1993;609-630.
5. Veeze H.J., Halley D.J., Bijman J., de Jongste J.C., De Jonge H.R., Sinaasappel M. Determinants of mild clinical symptoms in cystic fibrosis patients. Residual chloride secretion measured in rectal biopsies in relation to the genotype. J. Clin. Invest. 93:1994;461-466.
6. Rozmahel R., Wilschanski M., Matin A., Plyte S., Oliver M., Auerbach W., Moore A., Forstner J., Durie P., Nadeau J., Bear C., Tsui L.C. Modulation of disease severity in cystic fibrosis transmembrane conductance regulator deficient mice by a secondary genetic factor. Nat. Genet. 12:1996;280-287.
7. Clarke L.L., Grubb B.R., Yankaskas J.R., Cotton C.U., McKenzie A., Boucher R.C. Relationship of a non-cystic fibrosis transmembrane conductance regulator-mediated chloride conductance to organ-level disease in Cftr(-/-) mice. Proc. Natl. Acad. Sci. USA. 91:1994;479-483.
8. Kent G., Iles R., Bear C.E., Huan L.J., Griesenbach U., McKerlie C., Frndova H., Ackerley C., Gosselin D., Radzioch D., O'Brodovich H., Tsui L.C., Buchwald M., Tanswell A.K. Lung disease in mice with cystic fibrosis. J. Clin. Invest. 100:1997;3060-3069.
9. Wallace D.P., Grantham J.J., Sullivan L.P. Chloride and fluid secretion by cultured human polycystic kidney cells. Kidney Int. 50:1996;1327-1336.
10. Tomich J.M., Wallace D., Henderson K., Mitchell K.E., Radke G., Brandt R., Ambler C.A., Scott A.J., Grantham J., Sullivan L., Iwamoto T. Aqueous solubilization of transmembrane peptide sequences with retention of membrane insertion and function (in process citation). Biophys. J. 74:1998;256-267.
11. Wallace D.P., Tomich J.M., Iwamoto T., Henderson K., Grantham J.J., Sullivan L.P. A synthetic peptide derived from glycine-gated Cl- channel induces transepithelial Cl- and fluid secretion. Am. J. Physiol. 272:1997;C1672-C1679.
12. Langosch D., Hartung K., Grell E., Bamberg E., Betz H. Ion channel formation by synthetic transmembrane segments of the inhibitory glycine receptor - a model study. Biochim. Biophys. Acta. 1063:1991;36-44.
13. Reddy G.L., Iwamoto T., Tomich J.M., Montal M. Synthetic peptides and four-helix bundle proteins as model systems for the pore-forming structure of channel proteins. II. Transmembrane segment M2 of the brain glycine receptor is a plausible candidate for the pore-lining structure. J. Biol. Chem. 268:1993;14608-14615.
14. + absorptive airways. Am. J. Physiol. 272:1997;L762-L771.
15. Van Scott M.R., Smith M.D., Welch C.A., Bentzel C., Metzger W.J. Bioelectric response of human nasal epithelial cells to polycationic protein. Am. J. Physiol. 271:1996;L159-L165.
16. Cunningham S.A., Awayda M.S., Bubien J.K., Ismailov I.I., Arrate M.P., Berdiev B.K., Benos D.J., Fuller C.M. Cloning of an epithelial chloride channel from bovine trachea. J. Biol. Chem. 270:1995;31016-31026.
17. Begenisich T., Melvin J.E. Regulation of chloride channels in secretory epithelia. J. Membr. Biol. 163:1998;77-85.
18. Mitchell K.E., Tomich J.M., Iwamoto T., Freeman L.C. Synthetic peptides derived from the brain glycine receptor form channels in planar lipid bilayers and increase chloride conductance in MDCK cells. Biophys. J. 74:1998;A388.
19. Mitchell K.E., Broughman J., Freeman L.C., Tomich J.M. Synthetic channel-forming peptides increase whole cell chloride conductance in IB3-1 cells. Biophys. J. 76:1999;A445.
20. Grantham J.J., Uchic M., Cragoe E.J.J., Kornhaus J., Grantham J.A., Donoso V., Mangoo-Karim R., Evan A., McAteer J. Chemical modification of cell proliferation and fluid secretion in renal cysts. Kidney Int. 35:1989;1379-1389.
21. Zeitlin P.L., Lu L., Rhim J., Cutting G., Stetten G., Kieffer K.A., Craig R., Guggino W.B. A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection. Am. J. Respir. Cell Mol. Biol. 4:1991;313-319.
22. Schwiebert E.M., Cid-Soto L.P., Stafford D., Carter M., Blaisdell C.J., Zeitlin P.L., Guggino W.B., Cutting G.R. Analysis of ClC-2 channels as an alternative pathway for chloride conduction in cystic fibrosis airway cells. Proc. Natl. Acad. Sci. USA. 95:1998;3879-3884.
23. Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflug. Arch. 391:1981;85-100.
24. Schwiebert E.M., Egan M.E., Hwang T.H., Fulmer S.B., Allen S.S., Cutting G.R., Guggino W.B. CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP. Cell. 81:1995;1063-1073.
25. Mueller P., Rudin D., Tien T.H., Westcott W.C. Reconstitution of excitable membrane structures in vitro. Circulation. 26:1962;1167-1171.
26. - channel and activation by phorbol ester. Am. J. Physiol. 274:1998;C455-C464.
27. Anderson M.P., Welsh M.J. Calcium and cAMP activate different chloride channels in the apical membrane of normal and cystic fibrosis epithelia. Proc. Natl. Acad. Sci. USA. 88:1991;6003-6007.
28. Gregory R.J., Cheng S.H., Rich D.P., Marshall J., Paul S., Hehir K., Ostedgaard L., Klinger K.W., Welsh M.J., Smith A.E. Expression and characterization of the cystic fibrosis transmembrane conductance regulator. Nature. 347:1990;382-386.
29. Fuller C.M., Benos D.J. CFTR! Am. J. Physiol. 263:1992;C267-C286.
30. Cid L.P., Montrose-Rafizadeh C., Smith D.I., Guggino W.B., Cutting G.R. Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues. Hum. Mol. Genet. 4:1995;407-413.
31. Bond T.D., Higgins C.F., Valverde M.A. P-glycoprotein and swelling-activated chloride channels. Methods Enzymol. 292:1998;359-370.
32. Roy G., Banderali U. Channels for ions and amino acids in kidney cultured cells (MDCK) during volume regulation. J. Exp. Zool. 268:1994;121-126.
33. Lang F., Paulmichl M. Properties and regulation of ion channels in MDCK cells. Kidney Int. 48:1995;1200-1205.
34. 2+-calmodulin-dependent protein kinase in human epithelial cells. J. Physiol. (Lond.). 488:1995;37-55.
35. Worrell R.T., Frizzell R.A. CaMKII mediates stimulation of chloride conductance by calcium in T84 cells. Am. J. Physiol. 260:1991;C877-C882.
36. - channels in colonic and parotid secretory cells. Am. J. Physiol. 274:1998;C161-C166.
37. Staley K., Smith R., Schaack J., Wilcox C., Jentsch T.J. Alteration of GABAA receptor function following gene transfer of the CLC-2 chloride channel. Neuron. 17:1996;543-551.
38. Tavakkol Z., Mitchell K.E., Ambler C.A., Stucky J., Iwamoto T., Tomich J.M. Biophysical characterization of M2GlyR peptides in lipid and aqueous environments. Biophys. J. A74:1998;388.
39. + channels. Biochim. Biophys. Acta, in press.
40. Drotos B., Mitchell K.E., Tomich J.M., Freeman L.C. Cytotoxic effects of synthetic channel-forming peptides on MDCK, T84 and IB3 cell lines. Biophys. J. A76:1999;442.