Desensitisation of protease-activated receptor-1 (PAR-1) in rat astrocytes: Evidence for a novel mechanism for terminating Ca2+ signalling evoked by the tethered ligand

Journal of Physiology

Volumn 525, Issue 2, 2000, Pages 319-330

  Ubl, Joachim J ^a   Sergeeva, Marina ^a,b   Reiser, Georg ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

BREFELDIN A; CALCIUM ION; CYCLOHEXIMIDE; FURA 2; LIGAND; PROTEINASE; PROTEINASE ACTIVATED RECEPTOR 1; SOYBEAN TRYPSIN INHIBITOR; THERMOLYSIN; THROMBIN; THROMBIN RECEPTOR ACTIVATING PEPTIDE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; ASTROCYTE; CALCIUM CURRENT; CELL GROWTH; CELL STIMULATION; CELL STRUCTURE; CELL SURVIVAL; CONTROLLED STUDY; DESENSITIZATION; DOSE RESPONSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; RAT; SIGNAL TRANSDUCTION; CALCIUM SIGNALING; INTERNALIZATION; NEWBORN; PROTEIN CLEAVAGE; PROTEIN SYNTHESIS; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION;

EID: 0034213673     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-7793.2000.00319.x     Document Type: Article

References (44)

1. BEECHER, K. L., ANDERSON, T. T., FENTON, J. W. II & FESTOFF, B. W. (1994). Thrombin receptor peptides induce shape change in neonatal murine astrocytes in culture. Journal uf Neuroscience Research 37, 108-115.
2. BÖHM, S. K., GRADY, E. F. & BUNNETT, N. W. (1997). Regulatory mechanisms that modulate signalling by G-protein-coupled receptors. Biochemical Journal 322, 1-18.
3. BRASS, L. F. (1992). Homologous desensitization of HEL cell thrombin receptors. Distinguishable roles for proteolysis and phosphorylation. Journal of Biological Chemistry 267, 6044-6050.
4. BRASS, L. F., MANNING, D. R., WILLIAMS, A. G., WOOLKALIS, M. J. & PONCZ, M. (1991). Receptor and G protein-mediated responses to thrombin in HEL cells. Journal of Biological Chemistry 266, 958-965.
5. BRASS, L. F., PIZARRO, S., AHUJA, M., BELMONTE, E., BLANCHARD, N., STADEL, J. M. & HOXIE, J. A. (1994). Changes in the structure and function of the human thromhin receptor during receptor activation, internalization, and recycling. Journal of Biological Chemistry 269, 2943-2952.
6. CAVANAUGH, K. P., GURWITZ, D., CUNNINGHAM, D. D. & BRADSHAW, R. A. (1990). Reciprocal modulation of astrocyte stellation by thrombin and protease nexin-1. Journal of Neurochemistry 54, 1735-1743.
7. CHEN, J., ISHII, M., WANG, L., ISHII, K. & COUGHLIN, S. R. (1994). Thrombin receptor activation. Confirmation of the intramolecular tethered liganding hypothesis and discovery of an alternative intermolecular branding mode. Journal of Biological Chemistry 269, 16041-16045.
8. CHEN, X., EARLEY, K., LUO, W., LIN, S.-H. & SCHILLLING, W. P. (1996). Functional expression of a human thrombin receptor in Sf9 insect cells: evidence for an active tethered ligand. Biochemical Journal 314, 603-611.
9. CORVERA, C. U., DÉRY, O., MCCONALOGUE, K., GAMP, P., THOMA, M., AL-ANI, B., CAUGHEY, G. H., HOLLENBERG, M. D. & BUNNETT, N. W. (1999). Thrombin and mast cell tryptase regulate guinea-pig myenteric neurons through proteinase-activated receptors-1 and -2. Journal of Physiology 517, 741-756.
10. DÉRY, O., CORVERA, C. U., STEINHOFF, M. & BUNNETT, N. W. (1998). Proteinase-activated receptors: novel mechanisms of signaling by serine proteases. American Journal of Physiology 43, C1429-1452.
11. DONOVAN, F. M., PIKE, C. J., COTMAN, C. W. & CUNNINGHAM, D. D. (1997). Thrombin induces apoptosis in cultured neurons and astrocytes via a pathway requiring tyrosine kinase and RhoA activities. Journal of Neuroscience 17, 5316-5326.
12. FENG, D. M., VEBER, D. F., CONNOLLY, T. M., CONDRA, C., TANG, M. J. & NUTT, R. F. (1995). Development of a potent thrombin receptor ligand. Journal of Medicinal Chemistry 38, 4125-4130.
13. GERSZTEN, R. E., CHEN, J., ISHII, M., ISHII, K., WANG, L., NANEVICZ, T., TURCK, C. W., VU, T. K. & COUGHLIN, S. R. (1994). Specificity of the thrombin receptor for agonist peptide is defined by its extracellular surface. Nature 368, 648-651.
14. GRAND, R. J. A., TURNELL, A. S. & GRABHAM, P. W. (1996). Cellular consequences of thrombin-receptor activation. Biochemical Journal 313, 353-368.
15. 2+ responses of protease-treated human platelets activated with α-thromhin and the tethered ligand peptide. Biochemistry 23, 915-921.
16. HAMMES, S. R. & COUGHLIN, S. R. (1999). Protease-activated receptor-1 can mediate responses to SFLLRN in thrombin-desensitized cells: Evidence for a novel mechanism for preventing or terminating signaling by PAR 1's tethered ligand. Biochemistry 38, 2486-2493.
17. HAMPRECHT, B. & LÖFFLER, F. (1985). Primary glial cultures as a model for studying hormone action. Methods in Enzymology 109, 341-345.
18. HEIN, L., ISHII, K., COUGHLIN, S. R. & KOBILKA, B. K. (1994). Intracellular targeting and trafficking of thrombin receptors. A novel mechanism for resensitization of a G protein-coupled receptor. Journal of Biological Chemistry 269, 27719-27726.
19. HOXIE, J. A., AHUJA, M., BELMONTE, E., PIZARRO, S., PARTON, R. & BRASS, L. F. (1993). Internalization and recycling of activated thrombin receptors. Journal of Biological Chemistry 268, 13756-13763.
20. ISHIHARA, H., CONNOLLY, A. J., ZENG, D., KAHN, M. L., ZHENG, Y. W., TIMMONS, C., TRAM, T. & COUGHLIN, S. R. (1997). Protease-activated receptor 3 is a second thrombin receptor in humans. Nature 386, 502-506.
21. ISHII, K., CHEN, J., ISHII, M., KOCH, W. J., FREEDMAN, N. J., LEFKOWITZ, R. J. & COUGHLIN, S. R. (1994). Inhibition of thrombin receptor signaling by a G-protein coupled receptor kinase. Functional specificity among G-protein coupled receptor kinases. Journal of Biological Chemistry 269, 1125-1130.
22. KAWABATA, A., .SAIFEDDINE, M., AL-ANI, B., LEBLOND, L. & HOLLENBERG, M. D. (1999). Evaluation of proteinase-activated receptor-1 (PAR(1)) agonists and antagonists using a cultured cell receptor desensitization assay: Activation of PAR(2) by PAR(1)-targeted ligands. Journal of Pharmacology and Experimental Therapeutics 288, 358-370.
23. KULIOPULOS, A., COVIC, L., SEELEY, S. K., SHERIDAN, P. J., HELIN, J. & COSTELLO, C. E. (1999). Plasmin desensitization of the PAR-1 thrombin receptor: Kinetics, sites of truncation, and implications for thrombolytic therapy. Biochemistry 38, 4572-4585.
24. MOLINO, M., BARNATHAN, E. S., NUMEROF, R., CLARK, J., DREVER, M., CUMASHI, A., HOXIE, J. A., SCHECHTER, N., WOOLKALIS, M. & BRASS, L. F. (1997). Interactions of mast cell tryptase with thrombin receptors and PAR-2. Journal of Biological Chemistry 272, 4043-4049.
25. NANEVICZ, T., ISHII, M., WANG, L., CHEN, M., CHEN, J., TURCK, C. W., COHEN, F. E. & COUGHLIN, S. R. (1995). .Mechanisms of thrombin receptor agonist specificity. Chimeric receptors and complementary mutations identify an agonist recognition site. Journal of Biological Chemistry 270, 21619-21625.
26. NORTON, K. J., SCARBOROUGH, R. M., KUTOK, J. L., ESCOBEDO, M. A., NANNIZI, L. & COLLER, B. S. (1993). Immunologic analysis of the cloned platelet thrombin receptor activation mechanism: evidence supporting receptor cleavage, release of the N-terminal peptide, and insertion of the tethered ligand into a protected environment. Blood 82, 2125-2136.
27. NYSTEDT, S., EMILSSON, K., WAHLESTEDT, C. & SUNDELIN, J. (1994). Molecular cloning of a potential proteinase activated receptor. Proceedings of the National Academy of Sciences of the USA 91, 9208-9212.
28. OFOSU, F. A., FREEDMAN, J., DEWAR, L., SONG, Y. & FENTON, J. W. II (1998). A trypsin-like platelet protease propagates protease-activated receptor-1 cleavage and platelet activation. Biochemical Journal 336, 283-285.
29. PIKE, C. J., VAUGHAN, P. J., CUNNINGHAM, D. D. & COTMAN, C. W. (1996). Thrombin attenuates neuronal cell death and modulates astrocytes reactivity induced by β-amyloid in vitro. Journal of Neurochemistry 66, 1374-1382.
30. 2+ mobilization. FEBS Letters 288, 123-128.
31. SELAK, M. A. (1994). Cathepsin G and thrombin: evidence for two different platelet receptors. Biochemical Journal 297, 269-275.
32. 2-terminal kinase groups of mitogen-activated protein kinases. Journal of Biological Chemistry 271, 5750-5754.
33. SMIRNOVA, I. V., ZHANG, S. X., CITRON, B. A., ARNOLD, P. M. & FESTOFF, B. W. (1998). Thrombin is an extracellular signal that activates intracellular death protease pathways inducing apoptosis in model motor neurons. Journal of Neurobiology 36, 64-80.
34. TURGEON, V. L. & HOUENOU, L. J. (1997). The role of thrombin-like (serine) proteases in the development, plasticity and pathology of the nervous system. Brain Research Reviews 25, 85-95.
35. TURGEON, V. L., LLOYD, E. D., WANG, S., FESTOFF, B. W. & HOUENOU, L. J. (1998). Thrombin perturbs neurite outgrowth and induces apoptotic cell death in enriched chick spinal motoneuron cultures through caspase activation. Journal of Neuroscience 18, 6882-6891.
36. UBL, J. J. & REISER, G. (1997). Characteristics of thrombin-induced calcium signals in rat astrocytes. Glia 21, 361-369.
37. UBL, J. J., VÖHRINGER, C. & REISER, G. (1998). Co-existence of two types of calcium-inducing protease-activated receptors (PAR-1 and PAR-2) in rat astrocytes and C6 glioma cells. Neuroscience 86, 597-609.
38. VAUGHAN, P. J., PIKE, C. J., COTMAN, C. W. & CUNNINGHAM, D. D. (1995). Thrombin receptor activation protects neurons and astrocytes from cell death produced by environmental insults. Journal of Neuroscience 15, 5389-5401.
39. VU, T. K., HUNG, D. T., WHEATON, V. I. & COUGHLIN, S. R. (1991). Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 64, 1057-1068.
40. WEIHONG, Y., TIRUPPATHI, C., LUM, H., QIAO, R. & MALIK, A. (1998). Protein kinase Cβ regulates heterologous desensitization of thrombin receptor (PAR-1) in endothelial cells. American Journal of Physiology 274, C387-395.
41. WEINSTEIN, J. R., GOLD, S. J., CUNNINGHAM, D. D. & GALL, C. M. (1995). Cellular localization of thrombin receptor mRNA in rat brain: expression by mesencephalic dopaminergic neurons and codistribution with prothrombin mRNA. Journal of Neuroscience 15, 2906-2919.
42. WEINSTEIN, J. R., LAU, A. L., BRASS, L. F. & CUNNINGHAM, D. D. (1998). Injury-related factors and conditions down-regulate the thrombin receptor (PAR-1) in a human neuronal cell line. Journal of Neurochemistry 71, 1034-1050.
43. WOOLKALIS, M. J., DEMELFI, T. M. JR, BLANCHARD, N., HOXIE, J. A. & BRASS, L. F. (1995). Regulation of thrombin receptors on human umbilical vein endothelial cells. Journal of Biological Chemistry 270, 9868-9875.
44. XU, W.-F., ANDERSEN, H., WHITMORE, T. E., PRESNELL, S. R., YEE, D. P., CHING, A., GILBERT, T., DAVIE, E. W. & FOSTER, D. C. (1998). Cloning and characterization of human protease-activated receptor 4. Proceedings of the National Academy of Sciences of the USA 95, 6642-6646.