Use of a dual firefly and Renilla luciferase reporter gene assay to simultaneously determine drug selectivity at human corticotrophin releasing hormone 1 and 2 receptors

Analytical Biochemistry

Volumn 281, Issue 2, 2000, Pages 187-192

  Parsons, S J W ^a   Rhodes, S A ^a   Connor, H E ^a   Rees, S ^a   Brown, J ^a   Giles, H ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLUMINESCENCE; CELL CULTURE; CONTROLLED DRUG DELIVERY; GENES;

COMPOUND SCREENINGS; CONCENTRATION-DEPENDENT; DRUG ACTIVITIES; FIREFLY LUCIFERASE; RENILLA LUCIFERASE; REPORTER GENE ASSAYS; STABLE CELL LINES; TRANSMEMBRANE RECEPTORS;

TARGETED DRUG DELIVERY;

ASTRESSIN; BUTYL[2,5 DIMETHYL 7 (2,4,6 TRIMETHYLPHENYL) 7H PYRROLO[2,3 D]PYRIMIDIN 4 YL]ETHYLAMINE; CORTICOTROPIN RELEASING FACTOR ANTAGONIST; CORTICOTROPIN RELEASING FACTOR DERIVATIVE; CORTICOTROPIN RELEASING FACTOR RECEPTOR; LUCIFERASE; RECEPTOR SUBTYPE; SAUVAGINE;

ANIMAL CELL; ARTICLE; BIOASSAY; CONCENTRATION RESPONSE; DRUG ACTIVITY; DRUG SELECTIVITY; LUMINESCENCE; NONHUMAN; PRIORITY JOURNAL; RECEPTOR UPREGULATION; REPORTER GENE;

ANIMALIA; RENILLA; RENILLA LUCIFERASE;

EID: 0034212465     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4570     Document Type: Article

References (17)

1. Milligan, G. (1993) Trends Pharmacol. Sci. 14, 239-244.
2. Katz, A., Wu, D., and Simon, M. I. (1992) Nature 360, 686-689.
3. Tang, W., and Gilman, G. (1991) Science 254, 1500-1503.
4. Bevan, N., Scott, S., Shaw, M. G., Marshall, F. H., and Rees, S. (1998) NeuroReport 9(12), 2703-2708.
5. Stratowa, C., Machat, H., Burger, E., Himmler, A., Schafer, R., Spevak, W., Weyer, U., Wichecastanon, M., and Czernilofsky, A. P. (1995) J. Recept. Signal Transduct. Res. 15(1-4), 617-630.
6. Stables, J., Green, A., Marshall, F., Fraser, N., Knight, E., Sautel, M., Milligan, G., Lee, M., and Rees, S. A. (1997) Anal. Biochem. 252(1), 115-126.
7. McDonnell, J., Latif, M. L., Rees, S., Bevan, N. J., and Hill, S. J. (1998) Br. J. Pharmacol. 125(4), 717-726.
8. Lorenz, W. W., McCann, R. O., Longiaru, M., and Cormier, M. J. (1988) Proc. Natl. Acad. Sci. USA 88, 4438-4442.
9. Schultz, D. W., Mansbach, R. S., Sprouse, J., Braselton, J. P., Collins, J., Corman, M., Dunaiskis, A., Faraci, S., Schmidt, A. W., Seeger, T., Seymour, P., Tingley, F. D., 3rd., Winston, E. N., Chen, Y. L., and Heym, J. (1996) Proc. Natl. Acad. Sci. USA 93(19), 10477-10482.
10. Stables, J., Scott, S., Brown, S., Roelant, C., Burns, D., Lee, M. G., and Rees, S. (1999) J. Recept. Signal Transduct. Res. 19, 395-410.
11. Chen, R., Lewis, K., Perrin, M. H., and Vale, W. W. (1993) Proc. Natl. Acad. Sci. USA 90, 8967-8971.
12. Lovenberg, T. W., Liaw, C. W., Grigoriadis, W., Clevenger, D. T., Chalmers, D. T., De Souza, E. B., and Oltersdorf, T. (1995) Proc. Natl. Acad. Sci. USA 92, 836-840.
13. Rees, S., Coote, J., Stables, J., Goodson, S., Harris, S., and Lee, M. G. (1996) Biotechniques 20(1), 102-104, 106, 108-110.
14. Lew, M. J., and Angus, J. A. (1995) Trends Pharmacol. Sci. 16, 328-337.
15. Roelant, C., Scheirer, W., and Burns, D. A. (1996) Biotechniques 20, 914-917.
16. Parsons, S. J. W., Connor, H. E., and Giles, H. (1999) Br. J. Pharmacol. 126, 120P.
17. Ardati, A., Goetschy, V., Gottowick, J., Henriot, S., Valdenaire, O., Deuschle, U., and Kilpatrick, G. (1999) Neuropharmacology 38, 441-448.