Proteomic analysis of T cell activation in the presence of cyclosporin A: Immunosuppressor and activator removal induces de novo protein synthesis

Molecular Immunology

Volumn 37, Issue 1-2, 2000, Pages 21-28

  Truffa Bachi, Paolo ^a   Lefkovits, Ivan ^b   Frey, Johann Rudolf ^b  

^a INSTITUT PASTEUR   (France)

^b BASEL INSTITUTE FOR IMMUNOLOGY   (Switzerland)

Author keywords

Cyclosporin A; Gene transcription; T lymphocyte activation

Indexed keywords

CONCANAVALIN A; CYCLOSPORIN A; IMMUNOSTIMULATING AGENT; IMMUNOSUPPRESSIVE AGENT; INTERLEUKIN 12; POLYPEPTIDE; PROTEOME;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FEMALE; GENE INDUCTION; GENETIC TRANSCRIPTION; IMMUNOSUPPRESSIVE TREATMENT; ISOTOPE LABELING; LYMPHOCYTE CULTURE; METABOLITE; MOUSE; NONHUMAN; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; RNA TRANSLATION; SIGNAL TRANSDUCTION; T LYMPHOCYTE ACTIVATION;

ANIMALS; CONCANAVALIN A; CYCLOSPORINE; FEMALE; GLYCOSYLATION; IMMUNOSUPPRESSIVE AGENTS; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED C57BL; PROTEIN BIOSYNTHESIS; T-LYMPHOCYTES;

ANIMALIA; MURINAE;

EID: 0034121321     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0161-5890(00)00026-2     Document Type: Article

References (16)

1. Anderson N.L., Anderson N.G. Analytical techniques for cell fractions. Part XXI: two-dimensionl analysis of serum and tissue protines: multiple isoletric focusing. Anal. Biochem. 85:1978;331-340.
2. Bemer V., Motta I., Perret R., Truffa-Bachi P. IL2 down modulates memory cells T-helper lymphocyte development during an antigenic stimulation in vitro. Eur. J. Immunol. 25:1995;3394-3401.
3. Bemer V., Truffa-Bachi P. T-cell activation by concanavalin a in the presence of cyclosporin a: immunosuppressor withdral induces NFATp translocation and IL2 gene transcription. Eur. J. Immunol. 26:1996;1481-1488.
4. Bierer B.E., Hollander G., Fruman D., Burakoff S.J. Cyclosporin-A and FK506 - molecular mechanisms of immunosuppression and probes for transplantation biology. Curr. Opin. Immunol. 5:1993;763-773.
5. Fruman D.A., Burakoff S.J., Bierer B.E. Immunophilins in protein folding and immunosupression. FASEB J. 8:1994;391-400.
6. Ho I.C., Hodge M.R., Rooney J.W., Glimcher L.H. The proto-oncogene c-maf is responsible for tissue-specific expression of interleukin-4. Cell. 85:1996;973-983.
7. Ho S., Clipstone N., Timmermann L., Northrop J., Graef I., Fiorentino D., Nourse J., Crabtree G.R. The mechanism of action of cyclosporin A and FK506. Clin. Immunol. Immunopathol. 80:1996;S40-S45.
8. Holt J. Fos and jun: inducible transcription factors requlating growth of normal and transformed cells. Cncer Treat. and Res. 63:1992;301-311.
9. Jain J., McCaffrey P.G., Valge-Archer V.E., Rao A. Nuclear factor of activated T cells contains Fos and jun. Nature. 356:1992;801-804.
10. Kettman J., Lefkovits I. An attempt to assess the overall diversity of murine T cells using two-dimensional gel electrophoresis. Eur. J. Immunol. 14:1984;769-777.
11. Lefkovits I., Young P., Kuhn L., Kettman J., Gemmell A., Tollaksen S., Anderson L., Anderson N. Use of large-scale two-dimensional ISODALT gel electrophoresis system in immunology. Immun. Met. 40:1985;401-410.
12. Lefkovits I., Frey J.R., Kuhn L., Kettman J.R., Behar G., Auffray C., Hoffmann J.P., Coleclough C. Human lymphocyte cDNA ordered library analyzed by 2D gel electrophoresis. Part 1: pooling strategy and matching of gel patterns. Appl. Theor. Electrophoresi. 5:1995;35-42.
13. O'Farrell P. High resolution two-dimensional electrophoresis of proteins. J. Biochem. Chem. 250:1975;4007-4021.
14. Rao A., Luo C., Hogan P.G. Transcription factors of the NFAT family - regulation and function. Annu. Rev. Immunol. 15:1997;707-747.
15. Rovira P. Mascarell L., Truffa-Bachi P., 2000. The impact of immunosuppressive drugs on the analysis of T-cell activation. Cur. Med. Chem 6, in press.
16. Soares H.D., Curran T., Morgan J.I. Transcription factors as molecular mediators in cell death. Ann. N.Y. Acad. Sci. 747:1994;172-182.