Potential false-positive results with antigen enhancement for immunohistochemistry of the p53 gene product in colorectal neoplasms

Journal of Pathology

Volumn 178, Issue 3, 1996, Pages 264-267

  Baas, Inge O ^a   Van den Berg, Frank M ^a   Mulder, Jan Willem R ^a   Clement, Marjon J ^a   Slebos, Robbert J C ^a   Hamilton, Stanley R ^b   Offerhaus, G Johan A ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

allelic loss; antigen enhancement; colorectal neoplasms; false positivity; immunohistochemistry; mutation; p53

Indexed keywords

PROTEIN P53;

ANTIGEN EXPRESSION; ARTICLE; CLINICAL ARTICLE; COLORECTAL CARCINOMA; CONTROLLED STUDY; DNA RESTRICTION; GENE MUTATION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL;

EID: 0029966127     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1096-9896(199603)178:3<264::aid-path485>3.0.co;2-%23     Document Type: Article

References (19)

1. Vogelstein B, Kinzler KW. p53 function and dysfunction. Cell 1992; 70: 523-526
2. Levine AJ. The p53 tumor suppressor gene. N Engl J Med 1992; 326: 1350-1351
3. Oren M. p53-the ultimate tumor suppressor gene FASEB J 1992; 6: 3169-3176
4. Levine AJ The p53 tumor suppressor gene and product. Cancer Surv 1992; 12: 59-80.
5. Lane DP p53, guardian of the genome. Nature 1992; 358: 15-16.
6. Finley CA. Hinos PW, Tan TH, et al Activating mutations for transformation by p53 produce a gene product that forms an hsc 700 p53 complex with an altered half-life Mol Cell Biol 1988; 8: 531-539.
7. Baas IO. Mulder JWR, Offerhaus GJA, Vogelstem B, Hamilton SR An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms. J Pathol 1994; 172: 5-12.
8. Hall PA, Lane DP p53 in tumor pathology can we trust immunohistochemistry? Revisited! J Pathol 1994; 172: 1-4.
9. Van den Berg FM, Baas IO, Polak MM, Offerhaus GJA. Detection of p53 overexpression in routinely fixed paraffin embedded tissue of human carcinomas, using a novel Target Unmasking Fluid (TUF). Am J Pathol 1993: 142: 381-385.
10. Cattoretti G, Pilen S. Parravicini C, et al. Antigen unmasking on formalinfixed, paraffin-embedded tissue sections J Pathol 1993; 171: 83-98.
11. Offerhaus GJA, de Feyter EP. Cornelisse CJ. et al The relationship of DNA aneuploidy to molecular genetic alterations in colorectal carcinoma Gastroenterology 1992; 102: 1612-1619.
12. DiGiuseppe JA, Ilruban RH, Offerhaus GJA, et al Detection of K-ras mutations in mucinous pancreatic duct hyperplasia from a patient with a family history of pancreatic carcinoma. Am J Pathol 1994; 144: 889-895.
13. Schuurman R, Keulen W Modified protocol for DNA Sequenase analysis using Sequenase 2 0 BioTechniques 1991; 10: 185.
14. Thor AD, Moore DH, Edgerton SM, et al. Accumulation of the p53 tumor suppressor gene protein an independent marker of prognosis in breast cancer J Natl Cancer Inst 1992: 84: 845-855
15. Allerd DR, Clark GM, Elledge R. et al. Association of p53 protein overexpression with tumor cell proliferation rate and clinical outcome in node-negative breast cancer. J Wall Cancer Inst 1993; 85: 200-206
16. Wynford-Thomas D p53 in tumor pathology: can we trust immunohistochemistry? J Pathol 1992, 166: 329-330
17. Maltzman W, Czyzyk L UV irradiation stimulates levels of p53 cellular tumour antigen in nontransformed mouse cells Mol Cell Biol 1984; 4: 1689-1694
18. Hall PA, McKee PH, Menage HD, et al High levels of p53 protein in UV irradiated normal human skin Oncogene 1993; 8: 203-207.
19. Lu X, Lane DP Differential induction of transcriptional active p53 following UV or ionizing radiation defects in chromosome instability syndromes? Cell 1993; 75: 765-778.