Evaluation of immunohistochemical staining of human duodenal endocrine cells after microwave antigen retrieval

Histochemical Journal

Volumn 29, Issue 2, 1997, Pages 177-181

  Nyhlin, Nils ^a   El Salhy, Magdy ^a   Sandström, Olof ^a   Suhr, Ole ^a  

^a UMEÅ UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOGRANIN A; CHROMOGRANIN B; GASTRIC INHIBITORY POLYPEPTIDE; GASTRIN; SECRETIN; SEROTONIN; SOMATOSTATIN;

ADULT; ARTICLE; DUODENUM; DUODENUM BIOPSY; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTESTINE EPITHELIUM; MALE; MICROWAVE RADIATION; NEUROSECRETORY CELL; NORMAL HUMAN; PRIORITY JOURNAL;

EID: 0030934144     PISSN: 00182214     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026441623791     Document Type: Article

References (14)

1. CATTORETTI, G., BECKER, M.H.G., KEY, G., DUCHROW, M., SCHLÚTER, C., CALLE, J. & GERDEST, J. (1992) Monoclonal antibodies against recombinant part of the Ki-67 antigen (MIB and MIB3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J. Pathol. 168, 357-63.
2. CUEVAS, E.C., BATEMAN, A.C., WILKINS, B.S., JOHNSON, P.A., WILLIAMS, J.H., LEE, A.H.S., JONES, D.B. & WRIGHT, D.H. (1994) Micowave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47, 448-52.
3. EL-SALHY, M., STENLING, R. & GRIMELIUS, L. (1993) Peptidergic innervation and endocrine cells in the human liver. Scand. J. Gastroenterol. 28, 809-15.
4. EVERS, P. & UYLINGS, H.B.M. (1994) Effects of microwave pretreatment on immunocytochemical staining of vibratom sections and tissue blocks of human cerebral cortex stored in formaldehyde fixative for long periods. J. Neurosci. Methods 55, 163-72.
5. HACKER, G.W., GRIMELIUS, L., DANSCHER, G., MUSS, W., ADAM, H. & THUNER, J. (1988) Silver acetate autometallography. An alternative enhancement technique for immunogold staining (IGSS) and silver amplification of gold, silver, mercury and zinc in tissue. J. Histotechnol. 11, 213-21.
6. HAZELBAG, H.M., BROK, L., VAN DORST, E., OFFERHAUS, G., FLEUREN, G. & HOGENDOORN, P. (1995) Immunostaining of chain-specific keratin on formalin-fixed, paraffin-embedded tissues: a comparison of various antigen retrieval systems using microwave heating and proteolytic pre-treatments. J. Histochem. Cytochem. 43, 429-37.
7. LANGLOIS, N.E., KING, G., HERRIOT, R. & THOMPSON, W.D. (1994) Non-enzymatic retrieval of antigen permits staining of follicle center cells by the rabbit polyclonal antibodies to protein gene product 9.5. J. Pathol. 173, 249-53.
8. MCKEE, P.H., HOBBS, C. & HALL, P.A. (1993) Antigen retrieval by microwave irradiation lowers immunohistochemical detection thresholds. Histopathology 23, 377-9.
9. SHI, S-R., KEY, M.E. & KLARA, K.L. (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39, 741-8.
10. SHI, S-R., IMAM, S., YOUNG, L., COTE, R.J. & TAYLOR, C.R. (1995) Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J. Histochem. Cytochem. 43, 193-201.
11. SUURMEIJER, A.J.H. & BOON, M.E. (1993) Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections. Eur. J. Morphol. 31, 144-50.
12. TAYLOR, C.R., SHI, S-R., CHAIWUN, B., YOUNG, L., IMAM, S.A. & COTE, R.J. (1994) Strategies for improving the immunohistochemical staining of various intracellular prognostic markers in formalin-paraffin sections. Hum. Pathol. 25, 263-70.
13. VON WASIELEWSKI, R., WERNER, M., NOTLE, M., WILKENS, L. & GEORGH, A. (1994) Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies. Histochemistry 102, 165-72.
14. YACHINS, A.T. & TROJANOWSKI, J.Q. (1994) Studies of childhood brain tumours using immunohistochemistry and microwave technology: methodological considerations. J. Neurosci. Methods 55, 191-200.