The γ2 subunit of the GABA(A) receptor is concentrated in synaptic junctions containing the α1 and β2/3 subunits in hippocampus, cerebellum and globus pallidus

Neuropharmacology

Volumn 35, Issue 9-10, 1996, Pages 1425-1444

  Somogyi, P ^a   Fritschy, J M ^b   Benke, D ^b   Roberts, J D B ^a   Sieghart, W ^c  

^a MRC Anat Neuropharmacology Unit   (United Kingdom)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Benzodiazepine receptor; Cerebellum; Electron microscopy; GABA(A) receptor; Globus pallidus; Hippocampus; Immunocytochemistry; Inhibition; Pyramidal cell; Subunit composition

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; BENZODIAZEPINE RECEPTOR; PEPTIDE ANTIBODY; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; RECEPTOR SUBUNIT;

ANIMAL TISSUE; ARTICLE; CELL TYPE; CEREBELLUM; CONTROLLED STUDY; DENDRITE; ELECTRON MICROSCOPY; GABAERGIC SYSTEM; GLOBUS PALLIDUS; GRANULE CELL; HIPPOCAMPUS; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE; INTERNEURON; MICROSCOPY; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; PURKINJE CELL; PYRAMIDAL NERVE CELL; RAT; SYNAPSE; TISSUE DISTRIBUTION;

ANIMALS; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CELL MEMBRANE; CEREBELLUM; GLOBUS PALLIDUS; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; INTERCELLULAR JUNCTIONS; MICROSCOPY, ELECTRON; NEURONS; PLASTIC EMBEDDING; RATS; RECEPTORS, GABA-A; SYNAPSES;

EID: 18144445554     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(96)00086-X     Document Type: Article

References (69)

1. Baude A., Nusser Z., Roberts J.D.B., Mulvihill E., McIlhinney R.A.J., Somogyi P. The metabotropic glutamate receptor (mGluR1α) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction. Neuron. 11:1993;771-787.
2. A-receptor in rat brain. J. Rec. Res. 11:1991;407-424.
3. A receptors containing the α1-subunit in rat brain demonstrated by immunoprecipitation and immunohistochemistry. Molec. Neuropharmac. 1:1991;103-110.
4. A receptors display association of γ2-subunit with α1- and β 2 3 -subunits J. Biol. Chem. 266:1991;4478-4483.
5. A receptor subtypes containing the δ-subunit in rat brain. Fedn Eur. Biochem. Socs Lett. 283:1991;145-149.
6. Benke D., Fritschy J.-M., Trzeciak A., Bannwarth W., Mohler H. Distribution, prevalence, and drug binding profile of γ-aminobutyric acid type A receptor subtypes differing in the β-subunit variant. J. Biol. Chem. 269:1994;27100-27107.
7. A-receptor subtypes differentiated by their γ-subunit variants: prevalence, pharmacology and subunit architecture. Neuropharmacology. 35:1996;1413-1422.
8. A-receptor subtypes in the spinal cord: an immunohistochemical study. J. Neurosci. 16:1996;283-297.
9. Buhl E.H., Halasy K., Somogyi P. Diverse sources of hippocampal unitary inhibitory postsynaptic potentials and the number of synaptic release sites. Nature. 368:1994;823-828.
10. A receptors. Brain Res. 603:1993;234-242.
11. - channel complex. J. Neurosci. 8:1988;602-614.
12. A receptor channels. J. Neurophysiol. 71:1994;1318-1335.
13. A receptor α-subunit iso-oligomers. J. Biol. Chem. 266:1991;24778-24784.
14. A receptor γ2 1-15 Cys antibodies. J. Neurochem. 58:1992;72-77.
15. A receptor isooligomers. J. Biol. Chem. 265:1990;3831-3835.
16. A/benzodiazepine receptor. J. Cell Biol. 110:1990;2043-2048.
17. Fritschy J.-M., Benke D., Mertens S., Oertel W.H., Bachi T., Mohler H. Five subtypes of type A γ-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunit-specific antibodies. Proc. natn. Acad. Sci. U.S.A. 89:1992;6726-6730.
18. A-receptor heterogeneity in the adult rat brain: differential regional and cellular distribution of seven major subunits. J. Comp. Neurol. 359:1995;154-194.
19. A-receptor subtypes during postnatal development: An immunohistochemical study. J. Neurosci. 14:1994;5302-5324.
20. A receptors containing the α1 subunit to neurochemically distinct subpopulations of rat hippocampal interneurons. Eur. J. Neurosci. 6:1994;837-853.
21. A-receptor subtypes: Differential association of the α1- and α3-subunit with serotonergic and GABAergic neurons. Neuroscience. 54:1993;881-892.
22. Gao B., Hornung J.P., Fritschy J.-M. Identification of distinct GABA(A)-receptor subtypes in cholinergic and parvalbumin-positive neurons of the rat and marmoset medial septum-diagonal band complex. Neuroscience. 65:1995;101-117.
23. Gray E.G. Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study. J. Anat. 93:1959;420-433.
24. A receptors in the rat retina. Visual Neurosci. 10:1993;551-561.
25. A receptor in the rat brain. J. Neurosci. 14:1994;7168-7179.
26. Gulyas A.I., Miles R., Sik A., Toth K., Tamamaki N., Freund T.F. Hippocampal pyramidal cells excite inhibitory neurons through a single release site. Nature. 366:1993;683-687.
27. Gunther U., Benson J., Benke D., Fritschy J.-M., Reyes G., Knoflach F., Crestani F., Aguzzi A., Arigoni M., Lang Y., Bluethmann H., Mohler H., Luscher B. Benzodiazepine-insensitive mice generated by targeted disruption of the γ2 subunit gene of γ-aminobutyric acid type A receptors. Proc. Natn. Acad. Sci. U.S.A. 92:1995;7749-7753.
28. Haring P., Stahli C., Schoch P., Takacs B., Staehelin T., Mohler H. Monoclonal antibodies reveal structural homogeneity of γ-aminobutyric acid/benzodiazepine receptors in different brain areas. Proc. Natn. Acad. Sci. U.S.A. 82:1985;4837-4841.
29. A receptors in the human hippocampal formation. J. Neurosci. 8:1988;1370-1383.
30. Jones M.V., Westbrook G.L. The impact of receptor desensitization on fast synaptic transmission. Trends Neurosci. 19:1996;96-101.
31. A/benzodiazepine receptors. J. Neurochem. 63:1994;1466-1476.
32. A/benzodiazepine receptor from rat cerebellum. J. Neurochem. 63:1994;371-374.
33. A receptor γ2-subunit in mice by alternative splicing. J. Neurochem. 56:1991;713-715.
34. A and glycine receptors in the mammalian retina. J. Neurosci. 16:1996;2127-2140.
35. A receptor subunit mRNAs in the rat brain. II. Olfactory bulb and cerebellum J. Neurosci. 12:1992;1063-1076.
36. A receptor subtypes immunopurified from rat brain with a subunit-specific antibodies have unique pharmacological properties. Neuron. 7:1991;667-676.
37. A receptor subtypes really occur in the brain. Trends Neurosci. 19:1996;139-143.
38. A receptor populations with novel subunit combinations and drug binding profiles identified in brain by α5- and δ-subunit-specific immunopurification. J. Biol. Chem. 268:1993;5965-5973.
39. A Receptors and Anxiety. From Neurobiology to Treatment (Biggio G., Sanna E., Serra M. and Costa E., Eds), pp. 41-56. Raven Press, New York.
40. 2 subunit cDNAs. Neurosci. Lett. 123:1991;265-268.
41. Naito S., Ueda T. Affinity-purified anti-protein I antibody. J. Biol. Chem. 256:1981;10657-10663.
42. A receptor determined by electron microscopic image analysis. J. Neurochem. 62:1994;815-818.
43. Nusser Z., Mulvihill E., Streit P., Somogyi P. Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience. 61:1994;421-427.
44. A receptor in relation to specific GABAergic synapses in the dentate gyrus Eur. J. Neurosci. 7:1995;630-646.
45. A receptors on cerebellar granule cells as determined by a quantitative immunogold method. J. Neurosci. 15:1995;2948-2960.
46. Nusser Z., Sieghart W., Benke D., Fritschy J.-M. and Somogyi P. (1996a) Differential synaptic localization of two major γ-aminobutyric acid type A receptor α subunits on hippocampal pyramidal cells. Proc. Natn. Acad. Sci. U.S.A. in press.
47. A receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J. Neurosci. 16:1996;103-114.
48. A and glutamate receptors in relation to transmitter release sites on the surface of cerebellar neurones. Progr. Brain Res. in press.
49. A receptor sub-units in the rat CNS. J. Comp. Neurol. 326:1992;193-216.
50. Peters A., Palay S.L., Webster H. (1991) The Fine Structure of the Nervous System. Oxford University Press, New York.
51. A receptor subpopulations. J. Biol. Chem. 268:1993;3753-3757.
52. A receptor subunit for benzodiazepine pharmacology. Nature. 338:1989;582-585.
53. Quirk K., Gillard N.P., Ragan C.I., Whiting P.J., McKernan R.M. γ-aminobutyric acid type A receptors in the rat brain can contain both γ2 and γ3 subunits, but γ1 does not exist in combination with another γ subunit. Molec. Pharmac. 45:1994;1061-1070.
54. A receptor subtypes expressed in rat cerebellum with respect to their α and γ/δ subunits. J. Biol. Chem. 269:1994;16020-16028.
55. A/benzodiazepine receptors: cellular and subcellular localization in the CNS with monoclonal antibodies. J. Neurosci. 7:1987;1866-1886.
56. A receptors and benzodiazepine receptors in the brain shown by monoclonal antibodies. Nature. 314:1985;168-171.
57. A receptor subtypes. Pharmac. Rev. 47:1995;181-234.
58. A receptors on channel function. Neuron. 5:1990;703-711.
59. - channel complex using monoclonal antibodies in the dorsal lateral geniculate nucleus of the cat. Eur. J. Neurosci. 2:1990;414-429.
60. Somogyi P., Hamori J. A quantitative electron microscopic study of the Purkinje cell axon initial segment. Neuroscience. 1:1976;361-366.
61. Somogyi P., Nunzi M.G., Gorio A., Smith A.D. A new type of specific interneuron in the monkey hippocampus forming synapses exclusively with the axon initial segments of pyramidal cells. Brain Res. 259:1983;137-142.
62. A receptors in the cerebellum of rat, cat, and monkey using monoclonal antibodies. J. Neurosci. 9:1989;2197-2209.
63. A-receptor immunoreactivity in the rat dorsal thalamus: an ultrastructural investigation. Neurosci. Lett. 158:1993;232-236.
64. A receptors. Biochem. J. 310:1995;1-9.
65. Todd A.J., Watt C., Spike R.C., Sieghart W. Colocalization of GABA, glycine, and their receptors at synapses in the rat spinal cord. J. Neurosci. 16:1996;974-982.
66. A receptor expressed in Xenopus oocytes requires 8 amino acids contained in the γ2L subunit. Neuron. 7:1991;27-33.
67. Waldvogel H.J., Faull R.L.M., Jansen K.L.R., Dragunow M., Richards J.G., Mohler H., Streit P. GABA, GABA receptors and benzodiazepine receptors in the human spinal cord: an autoradiographic and immunohistochemical study at the light and electron microscopic levels. Neuroscience. 39:1990;361-385.
68. A receptor subunit mRNAs in the rat brain. I. Telencephalon, diencephalon, mesencephalon J. Neurosci. 12:1992;1040-1062.
69. A-benzodiazepine receptor complex by immunoaffinity chromatography. Brain Res. 563:1991;325-328.