Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family

Molecular Membrane Biology

Volumn 17, Issue 1, 2000, Pages 47-57

  Meintanis, Christos ^a   Karagouni, Amalia D ^a   Diallinas, George ^a  

^a UNIVERSITY OF ATHENS   (Greece)

Author keywords

Mutagenesis; Purine; Structure function analysis; Transmembrane domain; Transport

Indexed keywords

ASCORBIC ACID; CARRIER PROTEIN; PURINE;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT; ARTICLE; ASPERGILLUS NIDULANS; CHIMERA; CONTROLLED STUDY; MEMBRANE TRANSPORT; MISSENSE MUTATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; STRUCTURE ACTIVITY RELATION;

ASPERGILLUS; BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; EUKARYOTA; MAMMALIA; METAZOA; PROKARYOTA;

EID: 0034105567     PISSN: 09687688     EISSN: None     Source Type: Journal    
DOI: 10.1080/096876800294489     Document Type: Article

References (33)

1. Bloch, J. C., Sychrova, H., Souciet, J. L., Jund, R. and Chevallier, M. R., 1992, Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates. Molecular Microbiology, 6, 2989-2997.
2. Cove, D. J., 1966, The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysics Acta, 113, 51-56.
3. Darlington, A. J. and Scazzocchio, C., 1967, Use of analogues and the substrate specificity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans. Journal of Bacteriology, 93, 937-940.
4. Diallinas, G. and Scazzocchio, C., 1989, A gene coding for the uric acid-xanthine permease of Aspergillus nidulans: inactivational cloning, characterisation and sequence of a cis-acting mutation. Genetics, 122, 341-350.
5. Diallinas, G., Gorfinkiel, L., Arst, H. N. Jr., Ceccheto, G. and Scazzochio, C., 1995, Genetic and molecular characterisation of a gene encoding a wide specificity purine permease of Aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. Journal of Biological Chemistry, 270, 8610-8622.
6. Diallinas G., Valdez, J., Sophianopoulou, V., Rosa, A. and Scazzocchio, C., 1998, Chimeric protein analysis reveals a region involved in function and specificity of purine transporters in the filamentous fungus Aspergillus nidulans conserved in bacteria, plants and metazoans. European Molecular Biology Organisation Journal, 17, 3827-3837.
7. Erion, M. D., Takabayashi, K., Smith, H. B., Kessi, J., Wagner, S., Honger, S., Shames, S. L. and Ealick, S. E., 1997, Purine nucleoside phosphorylase. 1. Structure-function studies. Biochemistry, 30, 11725-11734.
8. Faaland, C. A., Race, J. E., Ricken, G., Warner, F. J., Williams, W. J. and Holtzman, E. J., 1998, Molecular characterisation of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel family that is homologous to bacterial and Aspergillus nucleobase transporters. Biochimica et Biophysica Acta, 1442, 353-360.
9. Ferreira, T., Brethes, D., Napias, C. and Chevallier, J., 1997, Functional analyses of mutated purine-cytosine permease from Saccharomyces cerevisiae. A possible role of the hydrophilic segment 371-377 in the active carrier conformation. Journal of Biological Chemistry, 272, 9697-9702.
10. Ferreira, T., Chevallier, J., Paumard, P., Napias, C. and Brethes, D., 1999, Screening of intragenic second-site suppressor of purine-cytosine permease from Saccharomyces cerevisiae. Possible role of Ser272 in the base translocation process. European Journal of Biochemistry, 260, 22-30.
11. Gordon, S. E., Oakley, J. C., Varnum, M. D. and Zagotta, W. N., 1996, Altered ligand specificity by protonation in the ligand binding domain of cyclic nucleotide-gated channels. Biochemistry, 35, 3994-4001.
12. Gorfinkiel, L., Diallinas, G. and Scazzocchio, C., 1993, Sequence and regulation of the uapA gene encoding a uric acid-xanthine permease in the fungus Aspergillus nidulans. Journal of Biological Chemistry, 268, 23376-23381.
13. Guimaraes, J. M., Bazan, J. F., Zlotnik, A., Wiles, M. V., Grimaldi, F. L. and McClanahan, T., 1995, A new approach to the study of the haematopoitic development in the yolk sac of embryoid bodies. Development, 121, 3335-3346.
14. Hoffenberg, S., Nikolova, L., Pan, J. Y., Daniels, D. S., Wessling-Resnick, M., Knoll, B. J. and Dickey, B. F., 1995, Functional and structural interactions of the Rab5 D136N mutant with xanthine nucleotides. Biochemical and Biophysical Research Communications, 215, 441-249.
15. Kang, C., Sun, N., Hontzatko, R. B. and Fromm, H. J., 1994, Replacement of Asp333 with Asn by site-directed mutagenesis changes the substrate specificity of Escherichia coli adenylosuccinate synthetase from guanosine 5′-to xanthosine 5'-triphosphate. Journal of Biological Chemistry, 269, 24046-24049.
16. Kraupp, M. and Marz, R., 1995, Membrane transport of nucleobases: interaction with inhibitors. General Pharmacology, 26, 1185-1190.
17. Lockington, R. A., Sealy-Lewis, H. N., Scazzocchio, C. and Davies, R. W., 1985, Cloning and characterisation of the ethanol utilisation regulon in Aspergillus nidulans. Gene, 33, 137-149.
18. Loewen, S. K., Ng, A. M., Yao, S. Y., Cass, C. E., Baldwin, S. A. and Young, J. D., 1999, Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside-cotransporters hCNT1 and hCNT2. Journal of Biological Chemistry, 274, 24475-24484.
19. Rajan D. P., Huang, W., Dutta, B., Devoe, L. D., Leibach, F. H., Ganapathy, V. and Prasad, P. D., 1999, Human placental sodium-dependent vitamin C transporter (SVTC2): Molecular cloning and transport function. Biochemical Biophysical Research Communications, 262, 762-768.
20. Ravagnani, A., Gorfinkiel, L., Langdon, T., Diallinas, G., Adjaj, E., Demais, S., Gorton, D., Arst, H. N. Jr. and Scazzocchio, C., 1997, Subtle hydrophobic interactions between the seventh residue of the Zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA. European Molecular Biology Organisation Journal, 16, 3974-3986.
21. Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory).
22. Scazzocchio, C., 1994, The purine degradation pathway, genetics, biochemistry and regulation. In Genetics and Physiology of Aspergillus, S. Martinelli, and J. R. Kinghorn, eds (Amsterdam: Elsevier Science Publishers BV), pp. 221-257.
23. Scazzocchio, C. and Gorton, D. J., 1977, The regulation of purine breakdown. In Genetics and Physiology of Aspergillus, J. E. Smith and J. A. Pateman, eds (New York: Academic Press), pp. 255-265.
24. Scazzocchio, C., Sdrin, N. and Ong, G., 1982, Positive regulation in a eukaryote, a study of the uaY gene of Aspergillus nidulans: I. Characterisation of alleles, dominance and complementation studies and a fine structure map of the uaY-oxpA cluster. Genetics, 100, 185-208.
25. Schultes, N. P., Brutnell, T. P., Allen, A., Dellaporta, S. L., Nelson, T. and Chen, C., 1996, Leaf permease1 gene of maize is required for chloroplast development. The Plant Cell, 8, 463-475.
26. Stoeckler, J. D., Poirot, A. F., Smith, H. B., Parks, R. E. Jr, Ealick S. E., Takabayashi, K. and Erion, M. D., 1997 Purine nucleoside phosphorylase. 3. Reversal of purine base specificity by site-directed mutagenesis. Biochemistry, 30, 11749-11756.
27. Sunahara, R. K., Beuve, A., Tesmer, J. J. G., Sprang, S. R., Garbers, D. L. and Gilman, A. G., 1998, Exchange of substrate and inhibitor specificities between adenyl and guanyl cyclases. Journal of Biological Chemistry, 273, 16332-16338.
28. Tazebay, U. H., Sophianopoulou, V., Cubera, B., Scazzocchio C. and Diallinas, G., 1995, Post-transcriptional regulation and kinetic characterization of proline transport in germinating conidiospores of Aspergillus nidulans. FEMS Microbiology Letters, 132, 27-37.
29. Tsukaguchi, H., Tokui, T., Mackenzie, B., Berger, U. V., Chen, X. Z., Wang, Y., Brubaker, R. F. and Hediger, M. A., 1999, A family of mammalian Na+-dependent L-ascorbic acid transporters. Nature, 399, 70-75.
30. Tucker, C. L., Hurley, J. H., Miller, T. R., and Hurley, J. B., 1998, Two amino acid substitutions convert a guanyl cyclase, RetGC-1, into an adenyl cyclase. Proceedings of the National Academy of Sciences, USA, 95, 5993-5997.
31. Varnum, M. D., Black, K. D. and Zagotta, W. N., 1995, Molecular mechanism for ligand discrimination of cyclic nucleotide-gated channels. Neuron, 15, 619-625.
32. +-dependent nucleoside transporters. Journal of Biological Chemistry, 272, 28845-28848.
33. +-nucleoside transporter. Journal of Biological Chemistry, 274, 2298-2302.