Identification, purification, and molecular cloning of a putative plastidic glucose translocator

Plant Cell

Volumn 12, Issue 5, 2000, Pages 787-801

  Weber, Andreas ^a   Servaites, Jerome C ^b   Geiger, Donald R ^b   Kofler, Heike ^a   Hille, Diana ^a   Gröner, Ferdi ^a   Hebbeker, Ulrike ^a   Flügge, Ulf Ingo ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b UNIVERSITY OF DAYTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL PATHWAY; CELL MEMBRANE; CELL MEMBRANE TRANSPORT; CHLOROPLAST; CLONE; COMPLEMENTARY DNA; GLUCOSE TRANSLOCATOR; GLUCOSE TRANSPORTER; HEXOSE; MAIZE; POTATO; SPINACH; TOBACCO; TRANSPORT KINETICS;

ARABIDOPSIS; MAMMALIA; NICOTIANA TABACUM; SOLANUM TUBEROSUM; SPINACIA OLERACEA; ZEA MAYS;

EID: 0034094933     PISSN: 10404651     EISSN: None     Source Type: Journal    
DOI: 10.1105/tpc.12.5.787     Document Type: Article

References (72)

1. Baldwin, S.A., and Lienhard, G.E. (1989). Purification and reconstitution of glucose transporter from human erythrocytes. Methods Enzymol. 174, 39-50.
2. Bechtold, N., Ellis, G., and Pelletier, G. (1993). In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. 316, 1194-1199.
3. Beck, E. (1985). The degradation of transitory starch in chloroplasts. In Regulation of Carbon Partitioning in Photosynthetic Tissue, R.L. Heath and J. Preiss, eds (Baltimore, MD: Waverly), pp. 27-44.
4. Capaldi, R.A., and Vanderkooi, G. (1972). The low polarity of many membrane proteins. Proc. Natl. Acad. Sci. USA 69, 930-932.
5. Caspar, T., Huber, S.C., and Somerville, C. (1985). Alterations in growth, photosynthesis, and respiration in a starchless mutant of Arabidopsis thaliana (L) deficient in chloroplast phosphoglucomutase activity. Plant Physiol. 79, 11-17.
6. Caspar, T., Lin, T.-P., Kakefuda, G., Benbow, L., Preiss, J., and Somerville, C. (1991). Mutants of Arabidopsis with altered regulation of starch degradation. Plant Physiol. 95, 1181-1188.
7. Church, G.M., and Gilbert, W. (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991-1995.
8. Douce, R., and Joyard, J. (1982). Purification of the chloroplast envelope. In Methods of Chloroplast Molecular Biology, M. Edelman, R.B. Hallick, and N.-H. Chua, eds (Amsterdam, The Netherlands: Elsevier), pp. 239-256.
9. Dreses-Weringloer, U., Fischer, K., Wachter, E., Link, T.A., and Flügge, U.I. (1990). cDNA sequence of the precursor of the 37 kDa inner envelope membrane polypeptide from spinach chloroplasts: Its transit peptide contains an amphiphilic α-helix as the only detectable structural element. Eur. J. Biochem. 195, 361-368.
10. Eckerskorn, C., and Lottspeich, F. (1989). Internal amino acid sequence analysis of proteins separated by gel electrophoresis after tryptic digestion in polyacrylamide matrix. Chromatographia 28, 92-94.
11. Eckerskorn, C., Mewes, W., Goretzki, H., and Lottspeich, F. (1988). A new siliconized-glass fiber as support for protein chemical analysis of electroblotted proteins. Eur. J. Biochem. 176, 509-519.
12. Emanuelsson, O., Nielsen, H., and von Heijne, G. (1999). ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8, 978-984.
13. Fliege, R., Flügge, U.-I., Werdan, K., and Heldt, H.W. (1978). Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplasts. Biochim. Biophys. Acta 502, 232-247.
14. Flügge, U.-I., Fischer, K., Gross, A., Sebald, W., Lottspeich, F., and Eckerskorn, C. (1989). The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: Nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts. EMBO J. 8, 39-46.
15. Fondy, B.R., Geiger, D.R., and Servaites, J.C. (1989). Photosynthesis, carbohydrate metabolism and export in Beta vulgaris L. and Phaseolus vulgaris L. during square and sinusoidal light regimes. Plant Physiol. 89, 396-402.
16. Geiger, D.R., Shieh, W.-J., and Yu, X.-M. (1995). Photosynthetic carbon metabolism and translocation in wild-type and starch-deficient mutant Nicotiana sylvestris L. Plant Physiol. 107, 507-514.
17. Geiger, D.R., Servaites, J.C., and Fuchs, M.A. (2000). Role of starch in carbon translocation and partitioning at the plant level. Aust. J. Plant Physiol., in press.
18. Gerhardt, R., Stitt, M., and Heldt, H.W. (1987). Subcellular metabolite levels in spinach leaves. Plant Physiol. 83, 399-407.
19. Gleixner, G., Scrimgeour, C., Schmidt, H.-L., and Viola, R. (1998). Stable isotope distribution in the major metabolites of source and sink organs of Solarium tuberosum L.: A powerful tool in the study of metabolite partitioning in intact plants. Planta 207, 241-245.
20. Gould, G.W., and Bell, G.I. (1990). Facultative glucose transporters: An expanding family. Trends Biochem. Sci. 15, 18-23.
21. Gross, A., Brückner, G., Heldt, H.W., and Flügge, U.-I. (1990). Comparison of the kinetic properties, inhibition and labelling of the phosphate translocators from maize and spinach mesophyll chloroplasts. Planta 180, 262-271.
22. Hanson, K.R., and McHale, N.A. (1988). A starchless mutant of Nicotiana sylvestris containing a modified plastid phosphoglucomutase. Plant Physiol. 88, 838-844.
23. Häusler, R.E., Schlieben, N.H., Schulz, B., and Flügge, U.-I. (1998). Compensation of decreased triose phosphate/phosphate translocator activity by accelerated starch turnover and glucose transport in transgenic tobacco. Planta 204, 366-376.
24. Heineke, D., Kruse, A., Flügge, U.I., Frommer, W.B., Riesmeier, J.W., Willmitzer, L., and Heldt, H.W. (1994). Effect of antisense repression of the chloroplast triose-phosphate translocator on photosynthetic metabolism in transgenic potato plants. Planta 193, 174-180.
25. Heldt, H.W. (1980). Measurement of metabolite movement across the envelope and of the pH in the stroma and the thylakoid space in intact chloroplasts. Methods Enzymol. 69, 604-613.
26. Henderson, P.J.F. (1991). Sugar transport proteins. Curr. Opin. Struct. Biol. 1, 590-601.
27. Henderson, P.J.F., and Macpherson, A.J.S. (1986). Assay, genetics, proteins, and reconstitution of proton-linked galactose, arabinose, and xylose transport systems of Escherichia coli. Methods Enzymol. 125, 387-429.
28. Herold, A., Leegood, R.C., McNeil, P.H., and Robinson, S.P. (1981). Accumulation of maltose during photosynthesis in protoplasts isolated from spinach leaves treated with mannose. Plant Physiol. 67, 85-88.
29. Higgins, D.G., Thompson, J.D., and Gibson, T.J. (1996). Using CLUSTAL for multiple sequence alignments. Methods Enzymol. 266, 383-402.
30. +, D-glucose transporter using 4-azidophlorizin. FEBS Lett. 130, 244-248.
31. Huber, S.C., and Hanson, K.R. (1992). Carbon partitioning and growth of a starchless mutant of Nicotiana sylvestris. Plant Physiol. 99, 1449-1454.
32. Kammerer, B., Fischer, K., Hilpert, B., Schubert, S., Gutensohn, M., Weber, A., and Flügge, U.-I. (1998). Molecular characterization of a carbon transporter in plastids of heterotrophic tissues: The glucose 6-phosphate/phosphate antiporter. Plant Cell 10, 105-117.
33. Keegstra, K., and Yousif, A.E. (1986). Isolation and characterization of chloroplast envelope membranes. Methods Enzymol. 118, 316-325.
34. Klip, A., Walker, D., Ransome, K.J., Schroer, D.W., and Lienhard, G.E. (1983). Identification of the glucose transporter in rate skeletal muscle. Arch. Biochem. Biophys. 226, 198-205.
35. Konieczny, A., and Ausubel, F.M. (1993). A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 4, 403-410.
36. Kyte, J., and Doolittle, R.F. (1982). A simple method for displaying the hydrophobic character of a protein. J. Mol. Biol. 157, 105-132.
37. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
38. Li, H., Sullivan, T.D., and Keegstra, K. (1992). Information for targeting to the chloroplastic inner envelope membrane is contained in the mature region of the maize Bt1-encoded protein. J. Biol. Chem. 267, 18999-19004.
39. Lockshin, A., and Burris, R.H. (1966). Solubilization and properties of chloroplast lamellar protein. Proc. Natl. Acad. Sci. USA 56, 1564-1570.
40. Logemann, J., Schell, J., and Willmitzer, L. (1987). Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163, 16-20.
41. Lowry, O.H., and Passonneau, J.V. (1972). A Flexible System of Enzymatic Analysis. (New York: Academic Press).
42. 2 concentrations on leaf content of ribulose-1,5-bisphosphate carboxylase/oxygenase and intracellular distribution of soluble carbohydrates in tobacco, snapdragon, and parsley. Plant Physiol. 115, 241-248.
43. Page, R.D.M. (1996). TREEVIEW: An application to display phylogenetic trees on personal computers. Comp. Appl. Biosci. 12, 357-358.
44. Piccioni, R., Bellemare, G., and Chua, N.-H. (1982). Methods of polyacrylamide gel electrophoresis in the analysis and preparation of plant polypeptides. In Methods of Chloroplast Molecular Biology, M. Edelman, R.B. Hallick, and N.-H. Chua, eds (Amsterdam, The Netherlands: Elsevier), pp. 985-1014.
45. Pohlmeyer, K., Soll, J., Steinkamp, T., Hinnah, S., and Wagner, R. (1997). Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane. Proc. Natl. Acad. Sci. USA 94, 9504-9509.
46. 2 fixation from sugar beet leaves containing calcium oxalate. Photosynth. Res. 4, 281-287.
47. Rost, S., Frank, C., and Beck, E. (1996). The chloroplast envelope is permeable for maltose but not for maltodextrins. Biochim. Biophys. Acta 1291, 221-227.
48. 2 evolution in isolated chloroplasts. Plant Physiol. 78, 537-544.
49. Saier, M.H., Jr. (1998). Molecular phylogeny as a basis for the classification of transport proteins from bacteria, archaea and eukarya. Adv. Microbiol. Physiol. 40, 81-136.
50. Saitou, N., and Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
51. Schaefer, B.C. (1995). Revolutions in the rapid amplification of cDNA ends: New strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal. Biochem. 227, 255-273.
52. Schäfer, G., Heber, U., and Heldt, H.W. (1977). Glucose transport into spinach chloroplasts. Plant Physiol. 60, 286-289.
53. Schleucher, J., Vanderveer, P.J., and Sharkey, T.D. (1998). Export of carbon from chloroplasts at night. Plant Physiol. 118, 1439-1445.
54. Schmidt, R., and Dean, C. (1995). Hybridization analysis of YAC clones. Methods Mol. Cell. Biol. 5, 309-318.
55. Schmidt, R., Love, K., West, J., Lenehan, Z., and Dean, C. (1997). Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5. Plant J. 11, 563-572.
56. Servaites, J.C., Geiger, D.R., Tucci, M.A., and Fondy, B.R. (1989a). Leaf carbon metabolism and metabolite levels during a period of sinusoidal light. Plant Physiol. 89, 403-408.
57. Servaites, J.C., Fondy, B.R., Li, B., and Geiger, D.R. (1989b). Sources of carbon for export from spinach leaves throughout the day. Plant Physiol. 90, 1168-1174.
58. Shanahan, M.F. (1982). Cytochalasin B. A natural photoaffinity ligand for labeling the human erythrocyte glucose transporter. J. Biol. Chem. 257, 7290-7293.
59. Shanahan, M.F., Wadzinski, B.E., Lowndes, J.M., and Ruoho, A.E. (1985). Photoaffinity labeling of the human erythrocyte monosaccharide transporter with an aryl azide derivative of D-glucose. J. Biol. Chem. 260, 10897-10900.
60. Stitt, M. (1990). Fructose-2,6-bisphosphate as a regulatory metabolite in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 153-185.
61. Stitt, M., Wirtz, W., Gerhardt, R., Heldt, H.W., Spencer, C., Walker, D., and Foyer, C. (1985). A comparative study of metabolite levels in plant leaf material in the dark. Planta 166, 354-364.
62. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. (1997). The ClustaIX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882.
63. Trethewey, R.N., and ap Rees, T. (1994a). A mutant of Arabidopsis thaliana lacking the ability to transport glucose across the chloroplast envelope. Biochem J. 301, 449-454.
64. Trethewey, R.N., and ap Rees, T. (1994b). The role of the hexose transporter in the chloroplasts of Arabidopsis thaliana L. Planta 195, 168-174.
65. Veramendi, J., Roessner, U., Renz, A., Willmitzer, L., and Trethewey, R.N. (1999). Antisense repression of hexokinase 1 leads to an overaccumulation of starch in leaves of transgenic potato plants but not to significant changes in tuber carbohydrate metabolism. Plant Physiol. 121, 123-133.
66. Walmsley, A.R., Barett, M.P., Bringaud, F., and Gould, G.W. (1998). Sugar transporters from bacteria, parasites and mammals: Structure-activity relationships. Trends Biochem. Sci. 23, 476-481.
67. Weber, A., Menzlaff, E., Arbinger, B., Gutensohn, M., Eckerskorn, C., and Flügge, U.-I. (1995). The 2-oxoglutarate/malate translocator of chloroplast envelope membranes: Molecular cloning of a transporter protein containing a 12-helix motif and expression of the functional protein in yeast cells. Biochemistry 34, 2621-2627.
68. Wellner, M., Monden, I., and Keller, K. (1994). The role of cysteine residues in glucose-transporter-GLUT1-mediated transport and transport inhibition. Biochem. J. 299, 813-817.
69. Wiese, A., Gröner, F., Sonnewald, U., Deppner, H., Lerchl, J., Hebbeker, U., Flügge, U.I., and Weber, A. (1999). Spinach hexokinase I is located in the outer envelope membrane of plastids. FEBS Lett. 461, 13-18.
70. Wintermans, J.F., and DeMots, A. (1965). Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol. Biochim. Biophys. Acta 109, 448-453.
71. Zeeman, S.C., Northorp, F., Smith, A.M., and ap Rees, T. (1998a). A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme. Plant J. 15, 357-365.
72. Zeeman, S.C., Umemoto, T., Lue, W.L., Au-Yeung, P., Martin, C., Smith, A.M., and Chen, J. (1998b). A mutant of Arabidopsis lacking a chloroplastic isoamylase accumulates both starch and phytoglycogen. Plant Cell 10, 1699-1711.