Telomerase allows the immortalization of T antigen-positive DMD myoblasts: A new source of cells for gene transfer application

Gene Therapy

Volumn 7, Issue 7, 2000, Pages 619-623

  Seigneurin Venin, S ^a   Bernard, V ^a   Tremblay, J P ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

DMD myoblasts; Extension of life span; Large T antigen; Telomerase gene

Indexed keywords

T LYMPHOCYTE ANTIGEN; TELOMERASE; VIRUS LARGE T ANTIGEN;

ARTICLE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL PROLIFERATION; CONTROLLED STUDY; DUCHENNE MUSCULAR DYSTROPHY; ENZYME ACTIVITY; GENE TRANSFER; HUMAN; HUMAN CELL; MYOBLAST; PRIORITY JOURNAL; RETROVIRUS; SIMIAN VIRUS 40; TELOMERE;

ANIMALS; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CELL DIVISION; CELL LINE, TRANSFORMED; GENE EXPRESSION; GENE THERAPY; HUMANS; MICE; MUSCLE, SMOOTH; MUSCULAR DYSTROPHY, DUCHENNE; TELOMERASE; TRANSFECTION;

SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40; UNIDENTIFIED RETROVIRUS;

EID: 0034065647     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301132     Document Type: Article

References (20)

1. Webster C, Blau HM. Accelerated age-related decline in replicative life-span of Duchenne muscular dystrophy myoblasts: implications for cell and gene therapy. Somat Cell Mol Genet 1990; 16: 557-565.
2. Simon LV, Beauchamp JR, O'Hare M, Olsen I. Establishment of long-term myogenic cultures from patients with Duchenne muscular dystrophy by retroviral transduction of a temperature-sensitive SV40 large T antigen. Exp Cell Res 1996; 224: 264-271.
3. Mouly V et al. SV40 large T antigen interferes with adult myosin heavy chain expression, but not with differentiation of human satellite cells. Exp Cell Res 1996; 225: 268-276.
4. Counter CM et al. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J 1992; 11: 1921-1929.
5. Counter CM et al. Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes. J. Virol 1994; 68: 3410-3414.
6. Shay JW, Wright WE. Quantitation of the frequency of immortalization of normal human diploid fibroblasts by SV40 large T-antigen. Exp Cell Res 1989; 184: 109-118.
7. Ray FA, Kraemer PM. Iterative chromosome mutation and selection as a mechanism of complete transformation of human diploid fibroblasts by SV40 T antigen. Carcinogenesis 1993; 14: 1511-1516.
8. Montalto MC, Ray FA. Telomerase activation during the linear evolution of human fibroblasts to tumorigenicity in nude mice (published erratum appears in Carcinogenesis 1997 Jun; 18(6): 1281). Carcinogenesis 1996; 17: 2631-2634.
9. Greider CW, Blackburn EH. Telomeres, telomerase and cancer. Sci Am 1996; 274: 92-97.
10. Bodnar AG et al. Extension of life-span by introduction of telomerase into normal human cells. Science 1998; 279: 349-352.
11. Seigneurin-Venin S et al. Transplantation in Scid mouse of normal and DMD myoblasts expressing the telomerase gene. (Submitted).
12. Counter CM et al. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci USA 1998; 95: 14723-14728.
13. Zhu J, Wang H, Bishop JM, Blackburn EH. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening (see comments). Proc Natl Acad Sci USA 1999; 96: 3723-3728.
14. Halvorsen TL, Leibowitz G, Levine F. Telomerase activity is sufficient to allow transformed cells to escape from crisis. Mol Cell Biol 1999; 19: 1864-1870.
15. Miranda AF, Babiss LE, Fisher PB. Transformation of human skeletal muscle cells by simian virus 40. Proc Natl Acad Sci USA 1983; 80: 6581-6585.
16. Iujvidin S, Fuchs O, Nudel U, Yaffe D. SV40 immortalizes myogenic cells: DNA synthesis and mitosis in differentiating myotubes. Differentiation 1990; 43: 192-203.
17. Moisset PA et al. Transplantation of large T antigen-immortalized myoblasts. Gene Therapy (Submitted).
18. Hahn WC et al. Creation of human tumour cells with defined genetic elements. Nature 1999; 400: 464-468.
19. Kim NW et al. Specific association of human telomerase activity with immortal cells and cancer. Science 1994; 266: 2011-2015.
20. Allsopp RC et al. Telomere length predicts replicative capacity of human fibroblasts. Prof Natl Acad Sci USA 1992; 89: 10114-10118.