Disruption of six novel ORFs from Saccharomyces cerevisiae chromosome VII and phenotypic analysis of the deletants

Yeast

Volumn 16, Issue 7, 2000, Pages 621-630

  Escribano, M Victoria ^a   Mazón, María J ^a  

^a CSIC   (Spain)

Author keywords

Functional analysis; Gene disruption; Morphology; Nuclear protein

Indexed keywords

FUNGAL PROTEIN; GLYCEROL;

ARTICLE; CELL STRUCTURE; CHROMOSOME 7; CONTROLLED STUDY; CULTURE MEDIUM; GENE DELETION; GENE DISRUPTION; GENETIC ANALYSIS; HAPLOIDY; MATING; NONHUMAN; OPEN READING FRAME; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SPOROGENESIS;

SACCHAROMYCES CEREVISIAE;

EID: 0034056123     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0061(200005)16:7<621::aid-yea557>3.0.co;2-0     Document Type: Article

References (22)

1. Baudin A, Ozier-Kalogeropoulos O, Denouel A, Lacroute F, Cullin C. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res 21: 3329-3330.
2. Chu S, DeRisi J, Eisen M, Mulholland J, Botstein D, Brown PO, Herskowitz I. 1998. The transcriptional program of sporulation in budding yeast. Science 282: 699-705.
3. Daum G, Tuller G, Nemec T, Hrastnik C, Balliano G, Cattel L, Milla P, Rocco F, Conzelmann A, Vionnet C, Kelly DE, Kelly S, Schweizer E, Schuller HJ, Hojad U, Greiner E , Finger K. 1999. Systematic analysis of yeast strains with possible defects in lipid metabolism. Yeast 15: 601-614.
4. Escribano MV, Eraso P, Portillo F, Mazón MJ. 1996. Sequence analysis of a 14.6kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27 SSM1b a putative S-adenosylmethionine-dependent enzyme and six new open reading frames. Yeast 12: 887-892.
5. Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG. 1996. Life with 6000 genes. Science 274: 546-567.
6. Huang D, Moffat J, Wilson WA, Moore L, Cheng C, Roach PJ, Andrews B. 1998. Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pc18 and Pc110. Mol Cell Biol 18: 3289-3299.
7. Ito H, Fukuda Y, Kimura A. 1983. Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163-168.
8. Kagan R, Clarke S. 1994. Widespread occurrence of three sequence motifs in diverse S-adenosylmethionine-dependent methyltransferases suggests a common structure for these enzymes. Arch Biochem Biophys 310: 417-427.
9. Koonin EV, Bork P, Sander C. 1994. Yeast chromosome III: new gene functions. EMBO J 13: 493-503.
10. Kurjan J. 1993. The pheromone response pathway in Saccharomyces cerevisiae. Ann. Rev Genet 27: 147-179.
11. Loo S, Laurenson P, Foss M, Dillin A, Rine J. 1996. Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae. Genetics 141: 889-902.
12. Measday V, Moore L, Retnakaran R, Lee J, Donoviel M, Neiman AM, Andrews B. 1997. A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase. Mol Cel Biol 17: 1212-1223.
13. Nakai K, Kanehisa M. 1992. A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14: 897-911.
14. Niedenthal RK, Riles L, Johnston M, Hegemann JH. 1996. Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 12: 773-786.
15. Oliver SG. 1996. A network approach to the systematic analysis of yeast gene function. Trends Gene 12: 241-242.
16. Robbins J, Dilworth SM, Laskey RA, Dingwall C. 1991. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64: 615-623.
17. Rothstein RJ. 1991. Targeting disruption replacement and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 184: 281-301.
18. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York.
19. Sikorski RS, Hieter P. 1989. A system of shuttle vectors in yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
20. Sütterlin C, Escribano MV, Gerold P, Maeda Y, Mazón MJ, Kinosita T, Schwarz T, Riezman. H. 1998. S. cerevisiae GPIIO the functional homolog of human PIG-B, is required for GPI-anchor synthesis. Biochem J 332: 153-159.
21. Voet M, Defoor E, Verhasselt P, Riles L, Robben J, Volckaert G. 1997. The sequence of a nearly unclonable 22.8 kb segment on the left arm of chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13: 177-182.
22. Wach A, Brachat A, Pohlmann R, Philippsen P. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.