Formation of dimeric Fabs in Escherichia coli: Effect of hinge size and isotype, presence of interchain disulphide bond, Fab' expression levels, tail piece sequences and growth conditions

Journal of Immunological Methods

Volumn 209, Issue 2, 1997, Pages 193-202

  Humphreys, David P ^a   Chapman, Andrew P ^a   Reeks, Dominic G ^a   Lang, Volker ^a   Stephens, Paul E ^a  

^a UCB PHARMA   (Belgium)

Author keywords

(Fab')2; Dimeric; Hinge sequence; Human; Isotype; Periplasmic antibody fragment expression; Tail piece

Indexed keywords

DIMER; IMMUNOGLOBULIN A; IMMUNOGLOBULIN F(AB')2 FRAGMENT; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN M;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; ESCHERICHIA COLI; FERMENTATION; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CYSTEINE; DIMERIZATION; DISULFIDES; ESCHERICHIA COLI; HUMANS; IMMUNOBLOTTING; IMMUNOGLOBULIN A; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN ISOTYPES; IMMUNOGLOBULIN M; MOLECULAR SEQUENCE DATA;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031469080     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00172-5     Document Type: Article

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