Comparison of marker protein expression in benign prostatic hyperplasia in vivo and in vitro

BJU International

Volumn 85, Issue 4, 2000, Pages 504-513

  Fry, P M ^a,c   Hudson, D L ^a   O'Hare, M J ^b   Masters, J R W ^a  

^a INSTITUTE OF UROLOGY AND NEPHROLOGY   (United Kingdom)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^c 3rd Floor Research Laboratories   (United Kingdom)

Author keywords

Cell culture; Epithelial; Immunohistochemistry; Prostatic hyperplasia; Stromal

Indexed keywords

CYTOKERATIN; PROTEIN; TISSUE ANTIGEN;

ARTICLE; CAMERA; CELL DIFFERENTIATION; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; CULTURE MEDIUM; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; PHENOTYPE; PRIORITY JOURNAL; PROSTATE HYPERTROPHY; PROTEIN EXPRESSION; SMOOTH MUSCLE FIBER; STROMA CELL;

CULTURE MEDIA, SERUM-FREE; EPITHELIAL CELLS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; PHENOTYPE; PROSTATIC HYPERPLASIA; STROMAL CELLS; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 0034049127     PISSN: 14644096     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1464-410X.2000.00458.x     Document Type: Article

References (39)

1. 1 Garraway WM, Collins GM, Lee RJ. High prevalence of benign prostatic hypertrophy in the community. Lancet 1991; 338: 469-47
2. 2 Peehl DM. Culture of human prostatic epithelial cells. In: Freshney RI, ed. Culture of Epithelial Cells. New York: Wiley-Liss, 1992: 159-80
3. 3 Kassen A, Sutkowski DM, Ahn H, Sansibar JA, Kozlowski JM, Lee C. Stromal cells of the human prostate: initial isolation and characterization. Prostate 1996; 28: 89-97
4. 4 Lechner JF, Shankar Narayan K, Ohnuki Y, Babcock MS, Jones LW, Kaighn ME. Replicative epithelial cell cultures from normal human prostate gland. J Natl Cancer Inst 1978; 60: 797-801
5. 5 McKeehan WL, Adams PS, Rosser MP. Direct mitogenic effects of insulin, epidermal growth factor, glucocorticoid, cholera toxin, unknown pituitary factors and possibly prolactin, but not androgen, on normal rat prostate epithelial cells in serum-free, primary cell culture. Cancer Res 1984; 44: 1998-2010
6. 6 Peehl DM, Stamey TA. Serum-free growth of adult human prostatic epithelial cells. In Vitro Cell Dev Biol 1986; 22: 82-90
7. 7 McKeehan WL, Adams P, Rosser MP. Modified nutrient medium MCDB151, defined growth factors, cholera toxin, pituitary factors and horse serum support epithelial cells and suppress fibroblast proliferation in primary cultures of rat ventral prostate cells. In Vitro Cell Dev Biol 1982; 18: 87-91
8. 8 Moll R, Franke WW, Schiller DL. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31: 11-24
9. 9 Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis. Sem Cancer Biol 1990; 1: 165-79
10. 10 Purkis PE, Steel JB, Mackenzie IC, Nathrath WBJ, Leigh IM, Lane EB. Antibody markers of basal cells in complex epithelia. J Cell Sci 1990; 97: 39-50
11. 11 Peehl DM, Leung GK, Wong ST. Keratin expression: a measure of phenotypic modulation of human prostatic epithelial cells by growth inhibitory factors. Cell Tiss Res 1994; 277: 11-8
12. 12 Troyanovsky SM, Guelstein VI, Tchipysheva TA, Krutovskikh VA, Bannikov GA. Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J Cell Sci 1989; 93: 419-26
13. 13 Peehl DM, Sellers RG, McNeal JE. Keratin 19 in the adult human prostate: tissue and cell culture studies. Cell Tissue Res 1996; 285: 171-6
14. 14 Bonkhoff H. Neuroendocrine cells in benign and malignant prostate tissue: morphogenesis, proliferation, and androgen receptor status. Prostate 1998; 8: 18-22
15. 15 Xue Y, Smedts F, Verhofstad A, Debruyne F, de la Rosette JJMCH, Schalken J. Cell kinetics of prostate exocrine and neuroendocrine epithelium and their differential interrelationship: new perspectives. Prostate 1998; 8: 62-73
16. 16 Ruizeweld de Winter JA, Trapman J, Vermey M, Mulder E, Zegers ND, van der Kwast TH. Androgen receptor expression in human tissues: an immunohistochemical study. J Histochem Cytochem 1991; 39: 927-36
17. 17 Zhang J, Hess MW, Thurnher M et al. Human prostatic smooth muscle cells in culture: estradiol enhances expression of smooth muscle cell-specific markers. Prostate 1997; 30: 117-29
18. 18 Peehl DM, Sellers RG. Induction of smooth muscle cell phenotype in cultured human prostatic stromal cells. Exp Cell Res 1997; 232: 208-15
19. 19 Janssen PJ, Brinkmann AO, Boersma WJ, van der Kwast TH. Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pretreatment. J Histochem Cytochem 1994; 42: 1169-75
20. 20 Robinson EJ, Collins AT, Robson CN, Neal DN. Effects of a new α - reductase inhibitor (epristeride) on human prostate cell cultures. Prostate 1997; 32: 259-65
21. 21 Atherton AJ, O'Hare MJ, Buluwela L et al. Ectoenzyme regulation by phenotypically distinct fibroblast sub-populations isolated from the human mammary gland. J Cell Sci 1994; 107: 2931-9
22. 22 Shi S-R, Chaiwun B, Young L, Cote RJ, Taylor CR. Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J Histochem Cytochem 1993; 41: 599-1604
23. 23 Ogden GR, Lunny D, Lane EB. Keratin antigen retrieval in oral mucosal biopsies using microwave processing. Histochem J 1996; 28: 875-82
24. 24 Entwistle A. A comparison between the use of a high-resolution CCD camera and 35mm film for obtaining coloured micrographs. J Microscopy 1998; 192: 81-9
25. 25 Bonkhoff H, Remberger K. Widespread distribution of nuclear androgen receptors in the basal cell layer of the normal and hyperplastic human prostate. Virchows Arch a Pathol Anat 1993; 422: 35-8
26. 26 Liu AY, True LD, LaTray L et al. Cell-cell interaction in prostate gene regulation and cytodifferentiation. Proc Natl Acad Sci USA 1997; 94: 10705-10
27. 27 Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C. Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 1990; 143: 167-71
28. 28 De Angeli S, Valenti F, Buoro S, Conconi MT, Parnigotto PP. Morphological features and kinetics of primary cultures of BPH tissue supported by TV1 medium. Ann Anat 1996; 178: 41-8
29. 29 Chopra DP, Grignon DJ, Joiakim A et al. Differential growth factor responses of epithelial cell cultures derived from normal prostate, benign prostatic hyperplasia, and primary prostate carcinoma. J Cell Physiol 1996; 169: 269-80
30. 30 Cussenot O, Berthon P, Cochand-Priollet B, Maitland NJ, Le Duc A. Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections. Exp Cell Res 1994; 214: 83-92
31. 31 Magin TM, Schroder R, Leitgeb S et al. Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates. J Cell Biol 1998; 140: 1441-51
32. 32 Smith CM, Ballard SA, Wyllie MG, Masters JRW. Comparison of testosterone metabolism in benign prostatic hyperplasia and human prostate cancer cell lines in vitro. J Steroid Biochem Molec Biol 1994; 50: 151-9
33. 33 Delos S, Carsol J-L, Ghazarossian E, Raynaud J-P, Martin P-M. Testosterone metabolism in primary cultures of human prostate epithelial cells and fibroblasts. J Steroid Biochem Molec Biol 1995; 55: 375-83
34. 34 Desmouliere A, Geinoz A, Gabbiani F, Gabbiani G. Transforming growth factor-β1 induces α-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 1993; 122: 103-11
35. 35 Owens GK. Regulation of differentiation of vascular smooth muscle cells. Physiol Rev 1995; 75: 487-517
36. 36 Shapiro E, Hartanto V, Lepor H. Anti-desmin vs. anti-actin for quantifying the area density of prostate smooth muscle. Prostate 1992; 20: 259-67
37. 37 Chamley-Campbell J, Campbell GR, Ross R. The smooth muscle cell in culture. Physiol Rev 1979; 59: 1-61
38. 38 Thyberg J. Differentiated properties and proliferation of arterial smooth muscle cells in culture. Int Rev Cytol 1996; 169: 183-265
39. 39 Peehl DM, Sellers RG. Basic FGF, EGF and PDGF modify TGFβ induction of smooth muscle cell phenotype in human prostatic stromal cells. Prostate 1998; 35: 4