Morphological features and kinetics of primary cultures of BPH tissue supported by TV1 medium

Annals of Anatomy

Volumn 178, Issue 1, 1996, Pages 41-48

  De Angeli, Sergio ^b   Valenti, Fabio ^a   Buoro, Sabrina ^b   Conconi, Maria Teresa ^a   Parnigotto, Pier Paolo ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b Ussl 9   (Italy)

Author keywords

Human hypertrophic prostate tissue; Kinetics; Morphology; Primary cultures in vitro

Indexed keywords

ACTIN; CYTOKERATIN;

AGED; ARTICLE; CELL CULTURE; CELL CYCLE S PHASE; CELL GROWTH; CELL KINETICS; CELL TYPE; CONTROLLED STUDY; EPITHELIOID CELL; FETAL CALF SERUM; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; MICROSCOPY; MITOSIS; PROSTATE BIOPSY; SCANNING ELECTRON MICROSCOPY; TIME;

EID: 0030027977     PISSN: 09409602     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0940-9602(96)80011-2     Document Type: Article

References (22)

1. Anselmo G, Fandella A, Maccatrozzo L, Merlo F, Bassi E, De Angeli S, Valenti F, Durante E, Nieri A, Furlan C (1989) Morfologia e cinetica cellulare del tessuto prostatico ipertrofico in coltura primaria: risultati preliminari. Acta Urol Ital 4: 235-240
2. Baserga R (1989) Cell growth and division. IRL Press, Oxford
3. Chapronierre D, McKeehan W (1986) Serial culture of single adult human prostatic epithelial cells in serum-free medium containing low calcium and a new growth factor from bovine brain. Cancer Res 46: 819-824
4. Chevalier S, Bleau G, Roberts K, Chapdelaine A (1981) Proliferation and differentiation of canine prostatic epithelial cells in culture. Mol Cell Endocrinol 24: 195-208
5. Cussenot O, Teillac P, Le Duc A, Ketels F, Calvo F (1989) Culture de cellules normales et tumorales de prostate humaine. Ann Urol 23: 533-537
6. De Angeli S, Fandella A, Conconi MT, Anselmo G, Parnigotto PP (1994) Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro. Prostate 25: 117-124
7. De Angeli S, Valenti F, Durante E, Bassani V, Parnigotto PP (1995) Primary cultures of human hypertrophic prostate tissue in WAJC 4O4 medium: A study of cell morphology and kinetics. Ann Anat 177: 185-192
8. Fandella A, De Angeli S, Anselmo G (1992) IPB: Attualità in tema di eziopatogenesi. Urologia 59: 13-20
9. Freshney R (1987) Culture of animal cells: a manual of basic technique. Alan R Liss Inc, NY, pp 107-126
10. Gatter KC, Dunnill MS, Van Muijen GN, Mason DY (1986) Human lung tumors may coexpress different classes of intermediate filaments. J Clin Pathol 39 (9): 950-954
11. Hamburger AW and Salmon SE (1977) Primary bioassay of human tumor stem cells. Science 197: 461-463
12. Lechner J, Babcock M, Marnell M, Shankar N, Kaighn M (1980) Normal human prostate epithelial cell cultures. Methods Cell Biol 21: 191-224
13. Makin CA, Bobrow LG, Bodmer WF (1984) Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol 37: 975-983
14. McKeehan W, Adams P, Rosser M (1982) Modified nutrient medium MCDB151, defined growth factors, cholera toxin, pituitary factors and horse serum support epithelial cells and suppress fibroblast proliferation in primary cultures of rat ventral prostate cells. In Vitro 18: 87-91
15. McKeehan W, Adams P, Rosser M (1984) Direct mitogenic effects of insulin, EGF, glucocorticoid, cholera toxin, unknown pituitary factors and possibly prolactin, but not androgen, on normal rat prostate epithelial cells in serum-free, primary cell culture. Cancer Res 44: 1998-2010
16. Nevalainen MT, Härkönen PL, Valve EM, Ping W, Nurmi M, Martikainen (1993) Hormone regulation of human prostate in organ culture. Cancer Res 53: 5199-5207
17. Peehl D, Wong S, Stamey T (1988) Clonal growth characteristics of human prostatic epithelial cells. In Vitro 24: 530-536
18. Peehl D, Wong S, Stamey T (1993) Vitamin A regulates proliferation and differentiation of human prostatic epithelial cells. Prostate 23: 69-78
19. Sherwood ER, Berg LR, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C (1990) Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 143: 167-171
20. Silvestrini R, Costa A, Veneroni S, Del Bino G, Persici P (1988) Comparative analysis of different approaches to investigate cell kinetics. Cell Tissue Kinet 21: 123-131
21. Syms A, Harper M, Bartersby S, Griffiths K (1982) Proliferation of human prostatic epithelial cells in culture: aspects of identification. J Urol 127: 561-567
22. Van der Poel HHG, Mulders PFA, Oosterhof GON, Schaafsma HE, Hendriks JCM, Schalken JA, Debruyne FMJ (1993) Prognostic value of karyometric and clinical characteristics in renal cell carcinoma. Cancer 72: 2667-2674