Overexpression, purification, and crystallization of the membrane-bound fumarate reductase from Escherichia coli

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 188-196

  Luna Chavez, César ^a,b   Iverson, Tina M ^c   Rees, Douglas C ^d   Cecchini, Gary ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^d California Institute of Technology   (United States)

Author keywords

Crystallography; Fumarate reductase; Membrane proteins; Succinate dehydrogenase

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; BUFFER; CITRATE SODIUM; CRYSTAL STRUCTURE; CRYSTALLIZATION; DETERGENT; ENZYME PURIFICATION; ENZYME SYNTHESIS; ENZYMIC DEGRADATION; ESCHERICHIA COLI; FUMARATE REDUCTASE; GEL FILTRATION; GENE OVEREXPRESSION; ION EXCHANGE CHROMATOGRAPHY; MACROGOL; PLASMID VECTOR; POLIDOCANOL; QUINOL; QUINONE OXIDOREDUCTASE; SUCCINIC ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034045853     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1238     Document Type: Article

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