Routine assessment of viability in split-thickness skin

Journal of Burn Care and Rehabilitation

Volumn 21, Issue 2, 2000, Pages 99-104

  Yang, H ^a   Jia, X M ^b   Acker, J P ^c   Lung, G ^c   McGann, L E ^c  

^a CANADIAN BLOOD SERVICES   (Canada)

^b 304TH HOSPITAL   (China)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

4 [3 (4 IODOPHENYL) 2 (4 NITROPHENYL) 2H 5 TETRAZOLIO] 1,3 BENZENE DISULFONATE; FORMAZAN; OXYGEN; TETRAZOLIUM; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; COLD INJURY; CONTROLLED STUDY; CRYOPRESERVATION; GRAFT SURVIVAL; INTERMETHOD COMPARISON; NONHUMAN; OXYGEN CONSUMPTION; QUALITY CONTROL; SKIN ALLOGRAFT; SPLIT THICKNESS SKIN GRAFT; VALIDATION PROCESS;

EID: 0034024378     PISSN: 02738481     EISSN: None     Source Type: Journal    
DOI: 10.1097/00004630-200021020-00004     Document Type: Article

References (21)

1. Brown JB, McDowell F. Massive repairs of burns with thick split-skin grafts: emergency "dressings" with homografts. Ann Surg 1942;115:658-74.
2. Seah CS. Skin graft and skin equivalent in burns. Ann Acad Med Singapore 1992;21:685-8.
3. Zieger MAJ, Tredget EE, McGann LE. Mechanisms of cryoinjury and cryoprotection in split-thickness skin. Cryobiology 1996;33:376-89.
4. Wachtel TL, Ninnemann J, Fisher JC, Frank, HA, Inancsi W. Viability of frozen allografts. Am J Surg 1979;138:783-7.
5. Taylor AC. Cryopreservation of skin: discussion and comments. Cryobiology 1966;3:192-6.
6. Bondoc CC, Burke JF. Clinical experience with viable frozen human skin and a frozen skin bank. Ann Surg 1971;3:371-81.
7. Zieger MAJ, Tredget EE, McGann LE. A simple, effective system for assessing viability in split-thickness skin with use of oxygen consumption. J Burn Care Rehabil 1993;14:310-18.
8. Klein MB, Shaw D, Barese S, Chapo GA, Cuono CB. A reliable and cost-effective in vitro assay of skin viability for skin banks and burn centers. J Burn Care Rehabil 1996;17:565-70.
9. May SR, DeClement FA. Skin banking. III: Cadaveric allograft skin viability. J Burn Care Rehabil 1981;2:128-41.
10. Ninnemann JL, Fisher JC. Skin and chorioamnion. In: Karow AM Jr, Pegg DE, editors. Organ preservation for transplantation. 2nd ed. New York: Marcel Dekker, Inc; 1981. p. 411-25.
11. Kearney JN, Wheldon LA, Gowland G. Cryopreservation of skin using a murine model: validation of a prognostic viability assay. Cryobiology 1990;27:24-30.
12. Ju X, Zhu Z, Li C. Viability and antigenic changes in the skin after preservation under different temperatures. Chin J Plast Surg Burns 1995;11:436-9.
13. Mosmann T. Rapid, colormetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
14. Yang H, Acker J, Chen A, McGann LE. In situ assessment of cell viability. Cell Transplant 1998;7:443-51.
15. Slater TF, Sawyer B, Strauli UD. Studies on succinate tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta 1963;77:383-93.
16. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, natural red and crystal violet. Biol Pharm Bull 1996;19:1518-20.
17. Fujiwara M, Nagao N, Monden K, Misumi M, Kageyama K, Yamamoto, K, et al. Enhanced protection against peroxidation-induced mortality of aortic endothelial cells by ascorbic acid-2-O-phosphate abundantly accumulated in the cell as the dephosphorylated form. Free Radic Res 1997;27:97-104.
18. Mgbonyebi OP, Russo J, Russo IH. Roscovitine inhibits the proliferative activity of immortal and neoplastic human breast epithelial cells. Anticancer Res 1998;18:751-5.
19. Cell Proliferation Reagent WST-1 [package insert]. Laval, Quebec: Boehringer Mannheim; 1998.
20. McGann LE, Yang H, Walterson M. Manifestation of cell damage after freezing and thawing. Cryobiology 1988; 25:178-85.
21. McGann LE, Walterson ML. Cryoprotection by dimethyl sulfoxide and dimethyl sulfone. Cryobiology 1987;24:11-6.