Initial predominance of the oxidative activity of type I 11β- hydroxysteroid dehydrogenase in primary rat Leydig cells and transfected cell lines

Journal of Andrology

Volumn 21, Issue 2, 2000, Pages 303-310

  Ge, Ren Shan ^a   Hardy, Matthew P ^a,b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b POPULATION COUNCIL   (United States)

Author keywords

CHOP cells; COS1 cells; Transfection

Indexed keywords

11BETA HYDROXYSTEROID DEHYDROGENASE; GLUCOCORTICOID RECEPTOR; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; LACTATE DEHYDROGENASE;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MECHANISM; GENETIC TRANSFECTION; LEYDIG CELL; MALE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; RAT; TESTOSTERONE SYNTHESIS;

11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 1; ANIMALS; CATALYSIS; CELL LINE; CULTURE MEDIA; DNA, COMPLEMENTARY; HYDROXYSTEROID DEHYDROGENASES; LEYDIG CELLS; MALE; OXIDATION-REDUCTION; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 0033993540     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Agarwal AK, Monder C, Eckstein B, White PC. Cloning and expression of rat cDNA encoding corticosteroid 11β-dehydrogenase. J Biol Chem. 1989;264: 18939-18943.
2. Agarwal AK, Monder C, White PC. Cloning of cDNA encoding an NAD(+)-dependent isoform of 11β-hydroxysteroid dehydrogenase in sheep kidney. Endocr Res. 1995;21:389-397.
3. Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS. Cloning and tissue distribution of the human 11β-hydroxysteroid dehydrogenase type 2 enzyme. Mol Cell Endocrinol. 1994;105:11-17.
4. Chrousos GP, Gold PW. The concepts of stress and stress system disorders. JAMA 1992;267:1244-1252.
5. Duperrex H, Kenouch S, Gaeggeler HP, Seckl JR, Edwards CR, Farman N, Rossier BC. Rat liver 11β-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid encodes oxoreductase activity in a mineralocorticoid-responsive toad bladder cell line. Endocrinology. 1993;132:612-619.
6. Ferguson SE, Michael AE, Cooke BA. The effects of different culture media and extracellular cofactor on 11βHSD activities, steroidogenesis and ATP levels in rat Leydig cells. In: Proceedings of The Endocrine Society's 21st Annual Meeting. San Diego, Calif; 1999:75.
7. Gao HB, Ge RS, Lakshmi V, Marandici, Hardy MP. Hormonal regulation of oxidative and reductive activities of 11β-hydroxysteroid dehydrogenase in rat Leydig cells. Endocrinology. 1997;138:156-161.
8. Gao HB, Shan LX, Monder C, Hardy MP. Suppression of endogenous corticosterone levels in vivo increases the steroidogenic capacity of purified rat Leydig cells in vitro. Endocrinology: 1996;137:1714-1718.
9. Ge RS, Gao HB, Nacharaju VL, Gunsalus GL, Hardy MP. Identification of a kinetically distinct activity of 11β-hydroxysteroid dehydrogenase in rat Leydig cells. Endocrinology. 1997;138:2435-2442.
10. Ge RS, Hardy DO, Catterall JF, Hardy MP. Developmental changes in glucocorticoid receptor and 11β-hydroxysteroid dehydrogenase oxidative and reductive activities in rat Leydig cells. Endocrinology. 1997;138:5089-5095.
11. Hales DB, Payne AH. Glucocorticoid-mediated repression of P450scc mRNA and de novo synthesis in cultured Leydig cells. Endocrinology. 1989;124:2099-2104.
12. Heffernan M, Dennis JW. Polyoma and hamster papovavirus large T antigen-mediated replication of expression shuttle vectors in Chinese hamster ovary cells. Nucleic Acids Res. 1990;19:85-92.
13. Jamieson PM, Chapman KE, Edwards CR, Seckl JR. 11β-hydroxysteroid dehydrogenase is an exclusive 11β-reductase in primary cultures of rat hepatocytes: effect of physicochemical and hormonal manipulations. Endocrinology. 1995;136:4754-4761.
14. Khanum A, Buczko E, Dufau ML. Essential role of adenosine triphophate in activation of 17β-hydroxysteroid dehydrogenase in the rat Leydig cells. Endocrinology. 1997;138:1612-1620.
15. Kletzien RF, Harris PK, Foellmi LA. Glucose-6-phosphate dehydrogenase: a housekeeping enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidant stress. FASEB J. 1994;8:174-181.
16. Klinefelter GR, Kelce WR, Hardy MP. Isolation and culture of Leydig cells from adult rats. Methods Toxicol. 1993;3A:166-181.
17. Lakshmi V, Monder C. Evidence for independent 11-oxidase and 11-reductase activities of 11β-hydroxysteroid dehydrogenase: enzyme latency, phase transitions, and lipid requirements. Endocrinology. 1985; 116:552-560.
18. Lakshmi V, Monder C. Purification and characterization of the corticosteroid 11β-dehydrogenase component of the rat liver 11β-hydroxysteroid dehydrogenase complex. Endocrinology. 1988;123:390-2398.
19. Leckie CM, Welberg LAM, Seckl JR. 11β-Hydroxysteroid dehydrogenase is a predominant reductase in intact rat Leydig cells. J Endocrinol. 1998;159:233-238.
20. Li KXZ, Smith RE, Ferrari P, Funder JW, Krozowski ZS. Rat 11β-hydroxysteroid dehydrogenase type 2 enzyme is expressed at low levels in the placenta and is modulated by adrenal steroids in the kidney. Mol Cell Endocrinol. 1996;120:67-75.
21. Low SC, Chapman KE, Edwards CR, Seckl JR. 'Liver-type' 11β-hydroxysteroid dehydrogenase cDNA encodes reductase but not dehydrogenase activity in intact mammalian COS-7 cells. J Mol Endocrinol. 1994;13:167-174.
22. Monder C, Miroff Y, Marandici A, Hardy MP. 11β-hydroxysteroid dehydrogenase alleviates glucocorticoid-mediated inhibition of steroidogenesis in rat Leydig cells. Endocrinology. 1994;134:1199-1204.
23. Nyska A, Leinger JR, Maronpot RR, Haseman JK, Hailey JR. Effect of individual versus groups caging on the incidence of pituitary and Leydig cell tumors in F344 rats: proposed mechanism. Med Hypotheses. 1998;50:525-529.
24. Ortlip SA, Li SA, Li JJ. Characterization of specific glucocorticoid receptor in the Syrian hamster testis. Endocrinology. 1981;109:1331-1338.
25. Pandolfi PP, Sonati F, Rivi R, Mason P, Grosveld F, Luzzatto L. Targeted disruption of the housekeeping gene encoding glucose 6-phosphate dehydrogenase (G6PD): G6PD is dispensable for pentose synthesis but essential for defense against oxidative stress. EMBO J. 1995;14: 5209-5215.
26. Park JW, Chun YS, Kim MS, Park YC, Kwak SJ, Park SC. Metabolic modulation of cellular redox potential can improve cardiac recovery from ischemia-reperfusion injury. Int J Cardiol. 1998;65:139-147.
27. Payne AH, Downing JR, Wong KL. Luteinizing hormone receptors and testosterone synthesis in two distinct population of Leydig cells. Endocrinology. 1980;106:1424-1429.
28. Phillips DM, Lakshmi V, Monder C. Corticosteroid 11β-dehydrogenase in rat testis. Endocrinology. 1989;125:209-216.
29. Powis G, Briehl M, Oblong J. Redox signaling and the control of cell growth and death. Pharmacol Ther. 1995;68:149-173.
30. Rajan V, Chapman KE, Lyons V, Jamieson P, Mullins JJ, Edwards CR, Seckl JR. Cloning, sequencing and tissue-distribution of mouse 11β-hydroxysteroid dehydrogenase-1 cDNA. J Steroid Biochem Mol Biol. 1995;52:141-147.
31. Roland BL, Funder JW. Localization of 11β-hydroxysteroid dehydrogenase type 2 in rat tissues - in situ studies. Endocrinology. 1996;137: 1123-1128.
32. Rusvai E, Naray-Fejes-Toth A. A new isoform of 11β-hydroxysteroid dehydrogenase in aldosterone target cells. J Biol Chem. 1993;268: 10717-10720.
33. Schultz R, Isola J, Parvinen M, Honkaniemi J, Wikstrom AC, Gustafsson JA, Pelto-Huikko M. Localization of the glucocorticoid receptor in testis and accessory sexual organs of male rats. Mol Cell Endocrinol. 1993;95:115-120.
34. Sokal RR, Rohlf FJ. Biometry. 3rd ed. New York: WH Freeman and Co; 1996.
35. Stalker A, Hermo L, Antakly T. Covalent affinity labeling, radioautography, and immunocytochemistry localize the glucocorticoid receptor in rat testicular Leydig cells. Am J Anat. 1989;186:369-377.
36. 2 for platelet-derived growth factor signal transduction. Science. 1995;270:296-299.
37. Yoshimoto K, Nakamura T, Ichihara A. Reciprocal effects of epidermal growth factor on key lipogenic enzymes in primary cultures of adult rat hepatocytes. Induction of glucose-6-phosphate dehydrogenase and suppression of malic enzyme and lipogenesis. J Biol Chem. 1983;258: 12355-12360.
38. Zhou ML, Gomez-Sanchez EP, Cox DL, Cosby D, Gomez-Sanchez CE. Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11β-hydroxysteroid dehydrogenase. Endocrinology. 1995;136:3729-3734.