Basal signaling activity of human dopamine D2L receptor demonstrated with an ecdysone-inducible mammalian expression system

Journal of Neuroscience Methods

Volumn 94, Issue 2, 2000, Pages 217-225

  Choi, Doo Sup ^a   Wang, Danxin ^a   Tolbert, Lara ^a   Sadée, Wolfgang ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Basal signaling activity; Dopamine D2 receptors; Ecdysone inducible system; Inverse agonist

Indexed keywords

CYCLIC AMP; DOPAMINE 2 RECEPTOR; ECDYSONE; ETICLOPRIDE; FLUPENTIXOL; FLUPHENAZINE; GUANINE NUCLEOTIDE BINDING PROTEIN; HALOPERIDOL; MURISTERONE A; PERTUSSIS TOXIN; PONASTERONE A; QUINPIROLE; SPIPERONE; TRIFLUOPERAZINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CONFOCAL MICROSCOPY; DRUG RECEPTOR BINDING; EMBRYO; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; KIDNEY CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR DENSITY; SIGNAL TRANSDUCTION;

CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ECDYSONE; ECDYSTERONE; HUMANS; RECEPTORS, DOPAMINE D2; SIGNAL TRANSDUCTION;

EID: 0033990602     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00147-8     Document Type: Article

References (32)

1. Allen L.F., Leflcowitz R.J., Caron M.G., Cotecchia S. G protein-coupled receptor genes as protooncogenes: Constitutively activating mutation of alpahlB-adrenergic receptor enhances mitogenesis and tumorigenicity. Proc. Natl. Acad. Sci. USA. 88:1991;11354-11358.
2. Arden J.R., Segredo V., Wang Z., Lameh J., Sadee W. Phosphorylation and agonist-specific intracellular trafficking of an epitope-tagged mu-opioid receptor expressed in HEK193 cells. J. Neurochem. 65:1995;1636-1645.
3. Baker E.L., Westphal R.S., Schmidt D., Sanders-Bush E. Constitutively active 5-HT2C receptors reveal novel inverse agonist activity of receptor ligands. J. Biol. Chem. 269:1994;11687-11690.
4. Boyson S.J., McGonigle P., Molinoff P.B. Quantitative autoradiographic localization of the D1 and D2 subtypes of dopamine receptors in rat brain. J. Neurosci. 6:1986;3177-3188.
5. Burford N.T., Tobin A.B., Nahorski S.R. Coupling of muscarinic m1, m2 and m3 acetylcholine receptors, expressed in Chinese hamster ovary cells, to pertussis toxin sensitive/insensitive guanine nucleotide-binding proteins. Eur. J. Pharmacol. 289:1995;343-351.
6. Chen C., Okayama H. High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:1987;2745-2752.
7. Dal Toso R., Sommer B., Ewert M., Herb A., Pirtchett D.B., Bach A., Shivers B.D., Seeburg P.H. The dopamine D2 receptor: Two molecular forms generated by alternative splicing. EMBO J. 8:1989;4025-4034.
8. Gilman A.G., Rall T.W., Nies A.S., Taylor P. The Pharmacological Basis of Therapeutics. 1990;383-435 Pergamon, New York.
9. Gingrich J.A., Caron M.G. Recent advances in the molecular biology of dopamine receptors. Annu. Rev. Neurosci. 16:1993;299-321.
10. Giros B., Sokoloff P., Martres M.P., Riou J.F., Emorine L.J., Schwartz J.C. Hyperlocomotion and indifference to cocaine and amphetamine in mice lacking the dopamine transporter. Nature. 342:1989;923-926.
11. Griffon N., Pilon C., Sautel F., Schwartz J.-C., Sokoloff P. Antipsychotics with inverse agonist activity at the dopamine D3 receptor. J. Neural Transm. 103:1996;1163-1175.
12. Hall D.A., Strange P.G. Evidence that antipsychotic drugs are inverse agonists at D2 dopamine receptors. Br. J. Pharmacol. 121:1997;731-736.
13. Högger P., Shockley M.S., Lameh J., Sadée W. Activating and inactivating mutations in N- and C-terminal i3 loop junctions of muscarinic acetylcholine Hm1 receptors. J. Biol. Chem. 270:1995;7405-7410.
14. Itokawa M., Toru M., Ito K., Tsuga H., Kameyama K., Haga T., Arinami T., Hamaguchi H. Sequestration of the short and long isoforms of dopamine D2 receptors expressed in Chinese hamster ovary cells. Mol. Pharmacol. 49:1996;560-566.
15. Khan Z.U., Mrzljak L., Gutierrez A., de la Calle A., Goldman-Rakic P.S. Prominence of the dopamine D2 short isoform in dopaminergic pathways. Proc. Natl. Acad. Sci. USA. 95:1998;7731-7736.
16. Kozell A.B., Neve K.A. Constitutive activity of a chimeric D2/D1 dopamine receptor. Mol. Pharmacol. 52:1997;1137-1149.
17. Liu Y.F., Civelli O., Grandy D.K., Albert P.R. Differential sensitivity of the short form and long human dopamine D2 receptor subtypes to protein kinase C. J. Neurochem. 59:1992;2311-2317.
18. 35S] binding. J. Pharmacol. Exp. Ther. 285:1998;119-126.
19. May D.C., Ross E.M. Rapid binding of guanosine 5′-O-(3-thiotriphosphate) to an apparent complex of beta-adrenergic receptor and the GTP-binding regulatory protein Gs. Biochemistry. 27:1988;4888-4893.
20. Milligan G., Bond R.A. Inverse agonism and the regulation of receptor number. Trends Pharmacol. Sci. 18:1997;468-474.
21. Milligan G., Bond R.A., Lee M. Inverse agonism: pharmacological curiosity or potential therapeutic strategy. Trends Pharmacol. Sci. 16:1995;10-13.
22. Missale C., Nash S.R., Robinson S.W., Jaber M., Caron M. Dopamine receptors: From structure to function. Physiol. Rev. 78:1998;189-225.
23. Monsma F.J., McVittie L.D., Gerfen C.R., Mahan L.C., Sibley D.R. Multiple D2 dopamine receptors produced by alternative RNA splicing. Nature. 342:1989;926-929.
24. Ng G.Y.K., O'Dowd B., Caron M., Dennis M., Brann M.R., George S.R. Phosphorylation and palmitoylation of the human D2L dopamine receptors in Sf9 cells. J. Neurochem. 63:1994;1589-1595.
25. Nilsson C.L., Ekman A., Hellstrand M., Eriksson E. Inverse agonism at D2 receptors: haloperidol-induced prolactin release from GH4C1 cells transfected with the human D2 receptor is antagonized by R(-)-n-propylnorapomorphine, raclopride, and phenoxybenzamine. Neuropsychopharmacology. 15:1996;53-61.
26. No D., Yao T.-P., Evans R.M. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci. USA. 93:1996;3346-3351.
27. Segredo V., Burford N.T., Lameh J., Sadee W. A constitutively internalizing and recycling mutant of the mu-opioid receptor. J. Neurochem. 68:1997;2395-2404.
28. Tang W.-J., Gilman A.G. Adenylyl cyclases. Cell. 70:1992;869-872.
29. Tiberi M., Caron M. High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype. J. Biol. Chem. 269:1994;27925-27931.
30. Tolbert L., Lameh J. Human muscarinic cholinergic receptor Hm1 internalizes via clathrin-coated vesicles. J. Biol. Chem. 271:1995;17335-17342.
31. Xia Z., Storm D.R. Calmodulin-regulated adenylyl cyclases and neuromodulation. Curr. Neurobiol. 7:1997;391-396.
32. Zhang L.J., Lachowicz J.E., Sibley D.R. The D2S and D2L dopamine receptor isoforms are differentially regulated in Chinese hamster ovary cells. Mol. Pharmacol. 45:1994;878-889.