An enhanced microfluidic chip coupled to an electrospray Qstar mass spectrometer for protein identification

Electrophoresis

Volumn 21, Issue 1, 2000, Pages 181-190

  Pinto, Devanand M ^a,b   Ning, Yuebin ^c   Figeys, Daniel ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b MDS SCIEX   (Canada)

^c Alberta Microelectronic Corporation   (Canada)

Author keywords

Microfluidics; Nonaqueous electrolyte; Polybrene; Protein digests; Proteomics

Indexed keywords

ELECTROLYTES; MASS SPECTROMETERS; MOLECULAR BIOLOGY; PROTEINS;

ANALYTICAL SYSTEMS; ELECTROSPRAYS; FLUIDIC SYSTEMS; MICROFABRICATED; MICROFLUIDIC-CHIPS; NON-AQUEOUS ELECTROLYTES; POLYBRENE; PROTEIN DIGESTS; PROTEIN IDENTIFICATION; PROTEOMICS;

MICROFLUIDICS;

AQUEOUS SOLUTION; ARTICLE; CELL LYSATE; DEVICE; DNA SEQUENCE; GENE EXPRESSION; MASS SPECTROMETRY; PHASE SEPARATION; PROTEIN ANALYSIS; RECOMBINANT DNA TECHNOLOGY;

EID: 0033987862     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000101)21:1<181::AID-ELPS181>3.0.CO;2-Q     Document Type: Article

References (41)

1. Blanchard, A., Genet. Eng. (NY) 1998, 20, 111-123.
2. Lipshutz, R. J., Morris, M. S., Chee, M., Hubbell, E., Kozal, M. J., Shah, N., Shen, N., Yang, R., Fodor, S. A., BioTechniques 1995, 19, 442-447.
3. Service, R. F., Science 1998, 282, 396-401.
4. Madden, S. L., Galella, E. A., Zhu, J. S., Bertelsen, A. H., Beaudry, G. A., Oncogene 1997, 15, 1079-1085.
5. Velculescu, V. E., Zhang, L., Vogelstein, B., Kinzler, K. W., Science 1995, 270, 484-487.
6. Wilkins, M. R., Pasquali, C., Appel, R. D., Ou, K., Golaz, O., Sanchez, J.-C., Yan, J. X., Gooley, A. A., Hughes, G., Humphery-Smith, I., Williams, K. L., Hochstrasser, D. F., BioTechnology 1996, 14, 61-65.
7. Cordwell, S. J., Basseal, D. J., Humphery-Smith, I., Electrophoresis 1997, 18, 1335-1346.
8. Fey, S. J., Nawrocki, A., Larsen, M. R., Görg, A., Roepstorff, P., Skews, G. N., Williams, R., Larsen, P. M., Electrophoresis 1997, 18, 1361-1372.
9. Figeys, D., Gygi, S. P., Zhang, Y., Watts, J., Gu, M., Aebersold, R., Electrophoresis 1998, 19, 1811-1818.
10. Garrels, J. I., McLaughlin, C. S., Warner, J. R., Futcher, B., Latter, G. I., Kobayashi, R., Schwender, B., Volpe, T., Anderson, D. S., Mesquita, F.-R., Payne, W. E., Electrophoresis 1997, 18, 1347-1360.
11. James, P., Biochem. Biophys. Res. Commun. 1997, 231, 1-6.
12. Link, A. J., Hays, L. G., Carmack, E. B., Yates III, J. R., Electrophoresis 1997, 18, 1314-1334.
13. O'Connor, C., Farris, M., Fowler, R., Qi, S., Electrophoresis 1997, 18, 1483-1490.
14. Ostergaard, M., Rasmussen, H. H., Nielsen, H. V., Vorum, H., Orntoft, T. F., Wolf, H., Celis, J. E., Cancer Res. 1997, 57, 4111-4117.
15. Shevchenko, A., Jensen, O. N., Podtelejnikov, A. V., Sagliocco, F., Wilm, M., Vorm, O., Mortensen, P., Shevchenko, A., Boucherie, H., Mann, M., PNAS 1996, 93, 14440-14445.
16. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
17. Klose, J., Kobalz, U., Electrophoresis 1995, 16, 1034-1059.
18. Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., Mann, M., Nature 1996, 379, 466-469.
19. Li, G., Waltham, M., Anderson, N. L., Unsworth, E., Treston, A., Weinstein, J. N., Electrophoresis 1997, 18, 3-4.
20. Patterson, S. D., Thomas, D., Bradshaw, R. A., Electrophoresis 1996, 17, 877-891.
21. Figeys, D., Ducret, A., Yates III, J. R., Aebersold, R., Nature Biotechn. 1996, 14, 1579-1583.
22. Figeys, D., Ducret, A., v. Oostveen, I., Aebersold, R., Anal. Chem. 1996, 68, 1822-1828.
23. Patterson, S. D., Aebersold, R., Electrophoresis 1995, 16, 1791-1814.
24. Haynes, P. A., Fripp, N., Aebersold, R., Electrophoresis 1998, 19, 939-946.
25. Desprez, T., Amselem, J., Caboche, M., Hofte, H., Plant J. 1998, 14, 643-652.
26. Manz, A., Graber, N., Widmer, M., Sensors Actuators B 1990, 1, 244-248.
27. Figeys, D., Ning, Y., Aebersold, R., Anal. Chem. 1997, 69, 3153-3160.
28. Figeys, D., Aebersold, R., Anal. Chem. 1998, 70, 3121-3127.
29. Figeys, D., Lock, C., Taylor, L., Aebersold, R., Rapid Commun. Mass Spectrom. 1998, 12, 1435-1444.
30. Ramsey, R. S., Ramsey, J. M., Anal. Chem. 1997, 69, 1174-1178.
31. Xue, Q. F., Foret, F., Dunayevskiy, Y. M., Zavracky, P. M., McGruer, N. E., Karger, B. L., Anal. Chem. 1997, 69, 426-430.
32. Xue, Q., Dunayevskiy, Y. M., Foret, F., Karger, B. L., Rapid Commun. Mass Spectrom. 1997, 11, 1253-1256.
33. Xu, N., Lin, Y., Hofstadler, S., Matson, D., Call, C., Smith, R., Anal. Chem. 1998, 70, 3553-3556.
34. Figeys, D., Aebersold, R., Electrophoresis 1997, 18, 360-368.
35. Figeys, D., Gygi, S. P., McKinnon, G., Aebersold, R., Anal. Chem. 1998, 70, 3728-3734.
36. Dolnik, V., Xu, D., Yadav, A., Bashkin, J., Marsh, M., Tu, O., Mansfield, E., Vainer, M., Madabhushi, R., Barker, D., Harris, D., J. Microcol. Sep. 1998, 10, 175-184.
37. th ASMS Conference on Mass Spectrometry and Allied Topics, June 1-5, 1997, Palm Springs, CA, p. 771.
38. Shevchenko, A., Chernushevich, I., Ens, W., Standing, K. G., Thomson, B., Wilm, M., Mann, M., Rapid Commun. Mass Spectrom. 1997, 11, 1015-1024.
39. Eng, J., McCormack, A. L., Yates III, J. R., J. Am. Soc. Mass Spectrom. 1994, 5, 976-989.
40. http://www.expasy.ch (Accession number P02666)
41. Figeys, D., Aebersold, R., J. Biomech. Eng. 1999, 121, 7-12.