Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli

Journal of Bacteriology

Volumn 179, Issue 11, 1997, Pages 3729-3735

  Hsing, Weihong ^a   Silhavy, Thomas J ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; ASPARTIC ACID; HISTIDINE; OUTER MEMBRANE PROTEIN; PHOSPHATASE; PHOSPHOTRANSFERASE; PORIN; REGULATOR PROTEIN; TYROSINE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DEPHOSPHORYLATION; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; OSMOREGULATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0030996163     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.11.3729-3735.1997     Document Type: Article

References (45)

1. Brissette, R. E., K. Tsung, and M. Inouye. 1991. Suppression of a mutation in OmpR at the putative phosphorylation center by a mutant EnvZ protein in Escherichia coli. J. Bacteriol. 173:601-608.
2. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104:541-555.
3. + mutant of EnvZ (EnvZ.N347D), a bifunctional signal transducer of Escherichia coli. J. Biol. Chem. 271:1424-1429.
4. Forst, S., and M. Inouye. 1988. Environmentally regulated gene expression for membrane proteins in Escherichia coli. Annu. Rev. Cell. Biol. 4:21-12.
5. Forst, S., J. Delgado, and M. Inouye. 1989. Phosphorylation of OmpR by the osmosensor EnvZ modulates expression of the ompF and ompC genes in Escherichia coli. Proc. Natl. Acad. Sci. USA 86:6052-6056.
6. Hall, M. N., and T. J. Silhavy. 1979. Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b. J. Bacteriol. 140:342-350.
7. Hall, M. N., and T. J. Silhavy. 1981. The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12. J. Mol. Biol. 146:23-43.
8. Hamilton, C. M., M. Aldea, B. K. Washburn, P. Babitzke, and S. R. Kushner. 1989. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-1622.
9. Hess, J. F., K. Oosawa, N. Kaplan, and M. I. Simon. 1988. Phosphorylation of three proteins in signaling pathway of bacterial chemotaxis. Cell 53:79-87.
10. Hoch, J. A. 1995. Control of cellular development in sporulating bacteria by the phosphorelay two-component signal transduction system, p. 129-144. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
11. Hsing, W., and T. J. Silhavy. Unpublished results.
12. Huang, K., and M. M. Igo. 1996. Identification of the bases in the ompF regulatory region, which interact with the transcription factor OmpR. J. Mol. Biol. 262:615-628.
13. Igo, M. M., L. Kenney, and T. J. Silhavy. Unpublished results.
14. Igo, M. M., and T. J. Silhavy. 1988. EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro. J. Bacteriol. 170:5971-5973.
15. Igo, M. M., A. J. Ninfa, J. B. Stock, and T. J. Silhavy. 1989. Phosphorylation and dephosphorylation of a bacterial transcription activator by a transmembrane receptor. Genes Dev. 3:1725-1734.
16. Jin, S., R. K. Prusti, T. Roitsch, R. G. Ankenbauer, and E. W. Nester. 1990. Phosphorylation of VirG protein of Agrobacterium tumefaciens by the autophosphorylated VirA protein: essential role in biological activity of VirG. J. Bacteriol. 172:4945-4950.
17. Jin, T., and M. Inouye. 1993. Ligand binding to the receptor domain regulates the ratio of kinase to phosphatase activities of the signaling domain of the hybrid Escherichia coli transmembrane receptor, Taz1. J. Mol. Biol. 232:484-492.
18. Kamberov, E. S., M. R. Atkinson, and A. J. Ninfa. 1994. Effect of mutations in Escherichia coli glnL (ntrB), encoding nitrogen regulator II (NRII or NtrB) on the regulated phosphatase activity involved in bacterial nitrogen regulation. J. Biol. Chem. 269:28294-28299.
19. Kanamaru, K., H. Aiba, and T. Mizuno. 1990. Transmembrane signal transduction and osmoregulation in Escherichia coli. I. analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR. J. Biochem. 108:483-487.
20. Keener, J., and S. Kustu. 1988. Protein kinase and phosphoprotein phosphatase activities of nitrogen regulatory proteins NTRB and NTRC of enteric bacteria: roles of the conserved amino-terminal domain of NTRC. Proc. Natl. Acad. Sci. USA 82:8453-8457.
21. Kenney, L., M. Bauer, and T. J. Silhavy. 1995. Phosphorylation-dependent conformational changes in OmpR, an osmoregulatory DNA-binding protein of Escherichia coli. Proc. Natl. Acad. Sci. USA 92:8866-8870.
22. Kim, S., M. R. Wilmes-Riesenberg, and B. L. Wanner. 1996. Involvement of the sensor kinase EnvZ in the in vivo activation of the response-regulator PhoB by acetyl phosphate. Mol. Microbiol. 22:135-147.
23. Little, J. W. 1993. LexA cleavage and other self-processing reactions. J. Bacteriol. 175:4943-4950.
24. Lukat, G. S., B. H. Lee, J. M. Mottonen, A. M. Stock, and J. B. Stock. 1991. Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis. J. Biol. Chem. 266:8348-8354.
25. Lukat, G. S., W. R. McCleary, A. M. Stock, and J. B. Stock. 1992. Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors. Proc. Natl. Acad. Sci. USA 89:718-722.
26. Makino, K., M. Shinagawa, M. Amemura, T. Kawamoto, M. Yamada, and A. Nakata. 1989. Signal transduction in phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins. J. Mol. Biol. 210:551-559.
27. McCleary, W. R. 1996. The activation of PhoB by acetylphosphate. Mol. Microbiol. 20:1155-1163.
28. Mizuno, T., and S. Mizushima. 1990. Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes. Mol. Microbiol. 4:1077-1082.
29. Ninfa, A. J., and B. Magasanik. 1986. Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli. Proc. Natl. Acad. Sci. USA 83:5909-5913.
30. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
31. Pratt, L. A., and T. J. Silhavy. 1995. Porin regulon of Escherichia coli, p. 105-127. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
32. Pratt, L. A., W. Hsing, K. E. Gibson, and T. J. Silhavy. 1996. From acids to osmZ: multiple factors influence synthesis of the OmpF and OmpC porins in Escherichia coli. Mol. Microbiol. 20:911-917.
33. Rabin, R. S., and V. Stewart. 1995. Dual sensors and dual response regulators interact to control nitrate-and nitrite-responsive gene expression in Escherichia coli, p. 233-252. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
34. 33a. Russo, F. D. 1992. Ph.D. thesis. Princeton University, Princeton, N.J.
35. Russo, F. D., and T. J. Silhavy. 1991. EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes. J. Mol. Biol. 222:567-580.
36. Silhavy, T. J., M. Berman, and L. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Slauch, J. M., and T. J. Silhavy. 1989. Genetic analysis of the switch that controls porin gene expression in Escherichia coli K-12. J. Mol. Biol. 210: 281-292.
38. Stock, A. M., D. C. Wylie, J. M. Mottonen, A. M. Lupus, E. G. Ninfa, A. J. Ninfa, C. E. Schutt, and J. B. Stock. 1988. Phospho-proteins involved in bacterial signal transduction. Cold Spring Harbor Symp. Quant. Biol. 53:49-57.
39. 2+-bound form of CheY and mechanism of phosphoryl transfer in bacterial chemotaxis. Biochemistry 32:13375-13380.
40. Stock, J. B., M. G. Surette, M. Levit, and P. Park. 1995. Two-component signal transduction systems: structure-function relationships and mechanisms of catalysis, p. 5-51. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction systems: structure-function relationships and mechanisms of catalysis, p. 5-51. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
41. Tokishita, S., A. Kojima, and T. Mizuno. 1992. Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-located protein kinase, EnvZ. J. Biochem. 111:707-713.
42. Wanner, B. L., and M. R. Wilmes-Riesenberg. 1992. Involvement of phosphotransacetylase, acetate kinase, and acetyl phosphate synthesis in control of the phosphate regulon in Escherichia coli. J. Bacteriol. 174:2124-2130.
43. Weiss, V., and B. Magasanik. 1988. Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli. Proc. Natl. Acad. Sci. USA 85:8919-8923.
44. Wright, G. D., T. R. Holman, and C. T. Walsh. 1993. Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147. Biochemistry 32:5057-5063.
45. Yang, Y., and M. Inouye. 1993. Requirement of both kinase and phosphatase activities of an Escherichia coli receptor (Taz1) for ligand-dependent signal transduction. J. Mol. Biol. 231:335-342.