Induction of apoptosis by mistletoe lectin I and its subunits. No evidence for cytotoxic effects caused by isolated A- and B-chains

International Journal of Biochemistry and Cell Biology

Volumn 32, Issue 3, 2000, Pages 317-326

  Vervecken, Wouter ^a   Kleff, Stefan ^a   Pfüller, Uwe ^a   Büssing, Arndt ^b  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

^b Krebsforschung Herdecke   (Germany)

Author keywords

Apo2.7; Apoptosis; B chain; Lectin; RIP II; Viscum

Indexed keywords

CELL SURFACE RECEPTOR; GALECTIN; PROTEIN SUBUNIT; RIBOSOME INACTIVATING PROTEIN; VISCUM ALBUM LECTIN;

ALPHA CHAIN; APOPTOSIS; ARTICLE; BETA CHAIN; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LECTIN BINDING; LEUKEMIA CELL LINE; LYMPHOCYTE; PROTEIN SYNTHESIS INHIBITION; SIGNAL TRANSDUCTION;

APOPTOSIS; CELL LINE; FLOW CYTOMETRY; HUMANS; LECTINS; LYMPHOCYTES; MISTLETOE; PLANT LECTINS; PLANT PREPARATIONS; PLANT PROTEINS; PLANTS, MEDICINAL; PROTEIN STRUCTURE, QUATERNARY; RIBOSOMES; TOXINS, BIOLOGICAL;

EID: 0033979708     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(99)00135-1     Document Type: Article

References (33)

1. Büssing A. Mistletoe therapy and immunological research. Anticancer Drugs. 8:(1):1997;S1-S65.
2. Stein G.M., Schietzel M., Büssing A. Mistletoe in immunology and the clinic (short review). Anticancer Res. 18:1998;3247-3257.
3. Franz H. Mistletoe lectins (2). Franz H. Advances in Lectin Research. 4:1990;33-45 Springer-Verlag, Berlin.
4. Büssing A., Schietzel M. Apoptosis-inducing properties of Viscum album L. extracts from different host trees correlate with their content of toxic mistletoe lectins. Anticancer Res. 19:1999;23-28.
5. Franz H. Mistletoe lectins and their A and B chains. Oncology. 43:1986;23-34.
6. Endo Y., Tsurugi K., Franz H. The site of action of the A-chain of mistletoe lectin I on eukaryotic ribosomes. The RNA N-glycosidase activity of the protein. FEBS Lett. 231:1988;378-380.
7. Janssen O., Scheffler A., Kabelitz D. In vitro effects of mistletoe extracts and mistletoe lectins. Cytotoxicity towards tumor cells due to the induction of programmed cell death (apoptosis). Arzneimittel-Forschung/Drug Res. 43:1993;1221-1227.
8. Büssing A., Suzart K., Bergmann J., Pfüller U., Schietzel M., Schweizer K. Induction of apoptosis in human lymphocytes treated with Viscum album L. is mediated by the mistletoe lectins. Cancer Lett. 99:1996;59-72.
9. Möckel B., Schwarz T., Zinke H., Eck J., Langer M., Lentzen H. Effects of mistletoe lectin I on human blood cell lines and peripheral blood cells. Cytotoxicity, apoptosis and induction of cytokines. Arzneimittel-Forschung/Drug Res. 47:1997;1145-1151.
10. Hostanska K., Hajto T., Weber K., Fischer J., Lentzen H., Sutterlin B., Saller R. A natural immunity-activating plant lectin, Viscum album agglutinin-I, induces apoptosis in human lymphocytes, monocytes, monocytic THP-1 cells and murine thymocytes. Nat. Immun. 15:1996-97;295-311.
11. lo) by the N-acetyl-D-galactosamine-specific lectin from Viscum album L. Cell Death and Diff. 5:1998;231-240.
12. Büssing A., Multani A.S., Pathak S., Pfüller U., Schietzel M. Induction of apoptosis by the N-acetyl-galactosamine-specific toxic lectin from Viscum album L. is associated with a decrease of nuclear p53 and Bcl-2 proteins and induction of telomeric associations. Cancer Lett. 130:1998;57-68.
13. Büssing A., Wagner M., Wagner B., Stein G.M., Schietzel M., Schaller G., Pfüller U. Induction of mitochondrial Apo2.7 molecules and generation of reactive oxygen-intermediates in cultured lymphocytes by the toxic proteins from Viscum album L. Cancer Lett. 139:1999;79-88.
14. Büssing A., Vervecken W., Wagner M., Wagner B., Pfüller U., Schietzel M. Expression of mitochondrial Apo2.7 molecules and caspase-3 activation in human lymphocytes treated with the ribosome-inhibiting mistletoe lectins and the cell membrane permeabilizing viscotoxins. Cytometry. 37:1999;. in press.
15. Bantel H., Engels I.H., Voelter W., Schulze-Osthoff K., Wesselborg S. Mistletoe lectin activates caspase-8/FLICE independently of death receptor signaling and enhances anticancer drug-induced apoptosis. Cancer Res. 59:1999;2083-2090.
16. Sandvig K., van Deurs B. Endocytosis, intracellular transport, and cytotoxic action of Shiga toxin and ricin. Physiol. Rev. 76:1996;949-966.
17. Sandvig K., van Deurs B. Endocytosis and intracellular transport of ricin: Recent discoveries. FEBS Lett. 452:1999;67-70.
18. Hajto T., Hostanska K., Frei K., Rordorf C., Gabius H.J. Increased secretion of tumor necrosis factors α, interleukin 1, and interleukin 6 by human mononuclear cells exposed to β-galactoside-specific lectin from clinically applied mistletoe extract. Cancer Res. 50:1990;3322-3326.
19. Perillo N.L., Uittenbogaart C.H., Nguyen J.T., Baum L.G. Galectin-1, an endogenous lectin produced by thymic epithelial cells, induces apoptosis of human thymocytes. J. Exp. Med. 185:1997;1851-1858.
20. Yang R.Y., Hsu D.K., Liu F.T. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. USA. 93:1996;6737-6742.
21. Wada J., Ota K., Kumar A., Wallner E.I., Kanwar Y.S. Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin. J. Clin. Invest. 99:1997;2452-2461.
22. Büssing A. Induction of apoptosis by the mistletoe lectins. A review on the mechanisms of cytotoxicity mediated by Viscum album L. Apoptosis. 1:1996;25-32.
23. Eifler R., Pfüller K., Göckeritz W., Pfüller U. Improved procedures for isolation and standardisation of mistletoe lectins and their subunits: Lectin patern of the European mistletoe. Basu J., Kundu M., Chakrabarti. Lectins: Biology, Biochemistry, Clinical Biochemistry. vol. 9:1993;144-151 Wiley Eastern, New Delhi.
24. Tonevitsky A.G., Rakhmanova V.A., Agapov II, Shamshiev A.T., Usacheva E.A., Prokoph'ev S.A., Denisenko O.N., Alekseev Yu O., Pfüller U. The interactions of anti-MLI monoclonal antibodies with isoforms of the lectin from Viscum album. Immunol. Lett. 44:1995;31-34.
25. Dean P.D.G., Johnson W.S., Middle F.A. Divinylsulphone-based coupling procedures. Rickwood D., Hames B.D. A Practical Approach - Affinity Chromatography. 1985;65 IRL Press Ltd, Oxford-Washington DC.
26. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
27. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
28. Johnson D., Gautsch J., Sportsman J., Elder J. Improved technique utilization nonfat dry milk for analysis of proteins and nucleic acids transfered to nitrocellulose. Gene Anal. Tech. 1:1984;3-8.
29. Sargent M.G. Fiftyfold amplification of the Lowry protein assay. Anal. Biochem. 163:1987;476-481.
30. Zhang C., Ao Z., Seth A., Schlossman S.F. A mitochondrial membrane protein defined by a novel monoclonal antibody is preferentially detected in apoptotic cells. J. Immunol. 157:1996;3980-3987.
31. Koester S.K., Roth P., Mikulka W.R., Schlossman S.F., Zhang C., Bolton W.E. Monitoring early cellular responses in apoptosis is aided by the mitochondrial membrane protein-specific monoclonal antibody APO2.7. Cytometry. 29:1997;306-312.
32. Franz H., Ziska P., Kindt A. A simple method for the preparation of the two different chains of the mistletoe lectin. Bog-Hansen T.C. Lectins: Biology, Biochemistry, Clinical Biochemistry. vol. 2:1982;771-776 Walter de Gruyter, Berlin-New York.
33. + T cell blasts. Immunol. Lett. 35:1993;125-134.