L-type voltage-dependent calcium channel α-1C subunit mRNA is present in ejaculated human spermatozoa

Molecular Human Reproduction

Volumn 6, Issue 2, 2000, Pages 127-136

  Goodwin, Leslie O ^a   Karabinus, David S ^b   Pergolizzi, Robert G ^a   Benoff, Susan ^a,c  

^a NORTH SHORE UNIVERSITY HOSPITAL   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

^c NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Calcium channels; RNA; RT PCR; Spermatozoa

Indexed keywords

CALCIUM CHANNEL; MESSENGER RNA;

ARTICLE; EJACULATION; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SPERM; TESTIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CALCIUM CHANNELS, L-TYPE; CONSERVED SEQUENCE; DNA PRIMERS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; MALE; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, QUATERNARY; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEMEN; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; SPERMATOZOA; TESTIS;

EID: 0033975082     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (84)

1. Adamopoulos, D.A., Pappa, A., Nicopoulou, S. et al. (1996) Seminal volume and total sperm number trends in men attending subfertility clinics in the Greater Athens area during the period 1977-1993. Hum. Reprod., 9, 1936-1941.
2. Arnoult, C., Lemos, J.R. and Florman, H.M. (1997) Voltage-dependent modulation of T-type calcium channels by protein tyrosine dephosphorylation. EMBO J., 16, 1593-1599.
3. Arnoult, C., Kazam, I.G., Visconti, P.E. et al. (1999) Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation. Proc. Natl. Acad. Sci. USA, 96, 6757-6762.
4. Auger, J., Kunstmann, J.M., Czyglik, F. et al. (1995) Decline in semen quality among fertile men in Paris during the past 20 years. N. Engl. J. Med., 332, 281-285.
5. Baba, T., Azuma, S., Kashiwabara, S. et al. (1994) Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. J. Biol. Chem., 269, 31845-31849.
6. Barratt, C.L.R. and St John, J.C. (1998) Diagnostic tools in male infertility. Hum. Reprod., 13 (Suppl., 1), 51-61.
7. Becker, S. and Berhane, K. (1997) A meta-analysis of 61 sperm count studies revisited. Fertil. Steril., 67, 1103-1108.
8. Benoff, S. (1997) Carbohydrates and fertilization: an overview. Mol. Hum. Reprod., 3, 599-637.
9. Benoff, S. (1998a) Voltage dependent calcium channels in mammalian spermatozoa. Frontiers Biosci., 3, d1220-d1240.
10. Benoff, S. (1998b) Modelling human sperm-egg interactions in vitro: signal transduction pathways regulating the acrosome reaction. Mol. Hum. Reprod., 4, 453-471.
11. Benoff, S. (1999) Receptors and channels regulating acrosome reaction. Hum. Fertil., 2, 42-55.
12. Benoff, S., Cooper, G.W., Paine, T. et al. (1999) Numerical dose-compensated IVF inseminations yield high fertilization and pregnancy rates. Fertil. Steril., 71, 1019-1028.
13. Berridge, M.J. (1993) Inositol trisphosphate and calcium signalling. Nature, 361, 315-325.
14. Blackmore, P.F. (1993) Thapsigarin elevates and potentiates the ability of progesterone to increase intracellular free calcium in human sperm: a possible role of perinuclear calcium. Cell Calcium. 14, 53-60.
15. Blackmore, P.F. and Eisoldt, S. (1999) The neoglycoprotein mannose-bovine serum albumin, but not progesterone, activates T-type calcium channels in human spermatozoa. Mol. Hum. Reprod., 5, 498-506.
16. Bolger, G.B., McPhee, I. and Houslay, M.D. (1996) Alternative splicing of cAMP-specific phosphodiesterase mRNA transcripts. J. Biol. Chem., 271, 1065-1071.
17. Carlsen, E., Giwercman, A., Keiding, N. et al. (1992) Evidence for decreasing quality of semen during the past 50 years. Br. Med. J., 305, 609-613.
18. Chiang, M.H., Stuerwald, N., Lambert, H. et al. (1994) Detection of human leukocyte antigen class I messenger ribonucleic acid transcripts in human spermatozoa via reverse transcription-polymerase chain reaction. Fertil. Steril., 61, 276-280.
19. Chirgwin, J, Przbyla, A., MacDonald, R. et al. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 18, 5294-5299.
20. Chomcznski, P and Sacchi, N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
21. Cooke, H.J., Hargreave, T. and Elliott, D.J. (1998) Understanding the genes involved in spermatogenesis. Fertil. Steril., 69, 989-995.
22. Cui, X., Li, H., Goradia, T.M. et al. (1989) Single-sperm typing: determination of the genetic distance between Gg-globin and parathyroid hormone loci using the polymerase chain reaction and allele-specific oligomers. Proc. Natl. Acad. Sci. USA, 86, 9389-9393.
23. Darszon, A., Labarca, P., Nishigaki, T. et al. (1999) Ion channels in sperm physiology. Physiol. Rev., 79, 481-510.
24. Eddy, E.M. and O'Brien, D.A. (1998) Gene expression during mammalian meiosis. Curr. Topics Dev. Biol., 37, 141-200.
25. Egozcue, J., Blanco, J. and Vidal, F. (1997) Chromosome studies in human sperm nuclei using fluorescence in-situ hybridization (FISH). Hum. Reprod. Update, 3, 441-452.
26. Esther, C.R., Howard, T.E., Marino, E.M. et al. (1996) Mice lacking angiotensin-converting enzyme have low blood-pressure, renal pathology, and reduced fertility. Lab. Invest., 7, 953-965.
27. Fisher, H.M., Brewis, I.A., Barratt, C.L.R. et al. (1998) Phosphoinositide 3-kinase is involved in the induction of the human sperm acrosome reaction downstream of tyrosine phosphorylation. Mol. Hum. Reprod., 4, 849-855.
28. Florman, H.M. (1994) Sequential focal and global elevations of sperm intracellular calcium are initiated by the zona pellucida during acrosomal exocytosis. Dev. Biol., 165, 152-164.
29. Gonzalez-Estrella, J.A., Coney, P., Ostash, P. et al. (1994) Dithiothreitol effects on the viscosity and quality of human semen. Fertil. Steril., 62, 1238-1243.
30. Goodwin, L.O., Leeds, N.B., Hurley, I. et al. (1997) Isolation and characterization of the primary structure of testis-specific L-type calcium channel: implications for contraception. Mol. Hum. Reprod., 3, 255-268.
31. Goodwin, L.O., Leeds, N.B., Hurley, I. et al. (1998a) Alternative splicing of exons of the a1 subunit of the rat testis L-type voltage-dependent calcium channel generates germ line-specific dihydropyridine binding sites. Mol. Hum. Reprod., 4, 215-226.
32. Goodwin, L.O., Leeds, N.B., Jacob, A. et al. (1998b) The unique structural diversity of the human testis-specific voltage-dependent calcium channel. [Abst.] J. Androl. Suppl., 26.
33. Goodwin, L.O., Leeds, N.B., Guzowski, D. et al. (1999a) Identification of structural elements of the testis-specific voltage dependent calcium channel that potentially regulate its biophysical properties. Mol. Hum. Reprod., 5, 311-322.
34. Goodwin, L.O., Leeds, N.B., Karabinus, D.S. et al. (1999b) Variability in L-type voltage-dependent calcium channel (L-VDCC) sequence in human testis and sperm. [Abstr. no. O-062.] In American Society for Reproductive Medicine, 55th Annual Meeting. Abstracts of the Scientific Oral and Poster Sessions. p. S24.
35. Innis, M., Gelfand, D. and Sninsky, J. (1990) PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., San Diego, CA, USA.
36. Johnson, M.D. (1998) Genetic risks of intracytoplasmic sperm injection in the treatment of male infertility: recommendations for genetic counseling and screening. Fertil. Steril., 70, 397-411.
37. Karabinus, D.S. and Gelety, T.J. (1997) The impact of sperm morphology evaluated by strict criteria on intrauterine insemination success. Fertil. Steril., 67, 536-541.
38. Kierszenbaum, A.L. and Tres, L.L. (1975) Structural and transcriptional features of the mouse spermatid genome. J. Cell. Biol., 65, 258-270.
39. 2+-channel alphal-subunit. Am. J. Physiol., 272, H1372-H1381.
40. Koch, W.J., Hui, A., Schull, G. et al. (1989) Characterization of cDNA clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta. FEBS Lett., 250, 386-388.
41. Koch, W.J., Ellinor, P.T. and Schwartz, A. (1990) cDNA cloning of a dihydropyridine-sensitive calcium channel from rat aorta: evidence for the existence of alternatively spliced forms. J. Biol. Chem., 265, 17786-17791.
42. Krege, J.H., John, S., Langenbach, L.L. et al. (1995) Male-female differences in fertility and blood-pressure in ACE deficient mice. Nature, 375, 146-148.
43. Kruger, T.F., Acosta, A.A., Siminons, K.F. et al. (1988) Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil. Steril., 49, 112-117.
44. Kumar, G., Patel, D. and Naz, R.K. (1993) c-MYC mRNA is present in human sperm cells. Cell. Mol. Biol. Res., 39, 111-117.
45. Kwon, Y.K. and Hecht, N.B. (1991) Cytoplasmic protein bindings to highly conserved sequences in the 3′ untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells. Proc. Natl. Acad. Sci. USA, 88, 3584-3588.
46. Lawrence, J.B. and Singer, R.H. (1986) Intracellular localization of messenger RNAs for cytoskeletal proteins. Cell, 45, 407-415.
47. 1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction. FEBS Lett., 388, 150-154.
48. Li, H., Gyllensten, U.B., Cui, X. et al. (1988) Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature, 335, 414-417.
49. Linares-Hernandez, L., Guzman-Grenfell, A.M., Hicks-Gomez, J.J. et al. (1998) Voltage-dependent calcium influx in human sperm assessed by simultaneous optical detection of intracellular calcium and membrane potential. Biochim. Biophys Acta, 1372, 1-12.
50. Liu, H. and Sperelakis, N. (1997) Tyrosine kinases modulate the activity of single L-type calcium channels in vascular smooth muscle cells from rat portal vein. Can. J. Physiol. Pharmacol., 75, 1063-1068.
51. ++/calmodulin-dependent protein-kinase and a male germ cell-specific calmodulin-binding protein are derived from the same gene. Mol. Cell. Biol., 11, 3960-3971.
52. 1 subunits can form low threshold small conductance channels with similarities to native T-type channels. Neuron, 20, 341-351.
53. Miller, D. (1997) RNA in the ejaculate spermatozoon: a window into molecular events in spermatogenesis and a record of the unusual requirements of haploid gene expression and post-meiotic equilibration. Mol. Hum. Reprod., 3, 669-676.
54. Miller, D., Tang, P.-Z., Skinner, T. et al. (1994) Differential RNA fingerprinting as a tool in the analysis of spermatozoal gene expression. Hum. Reprod., 9, 864-869.
55. Mosher, W.D. (1985) Reproductive impairments in the United States, 1965-1982. Demography, 22, 415-430.
56. Mullis, K.B. and Faloona, F.A. (1987) Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods Enzymol., 155, 335-350.
57. Palermo, G.D., Coehn, J., Alikani, M. et al. (1995) Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil. Steril., 63, 1231-1240.
58. Perez-Reyes, E., Cribbs, L.L., Daud, A. et al. (1998) Molecular characterization of a neuronal low-voltage-activated T-type calcium channel. Nature, 391, 896-900.
59. Pessot, C.A., Brito, M., Figueroa, J. et al. (1989) Presence of RNA in the sperm nucleus. Biochem. Biophys Res. Commun., 158, 272-278.
60. Piva, M. and Sharpe-Timmes, K.L. (1999) Peritoneal endometriotic lesions differentially express a haptoglobin-like gene. Mol. Hum. Reprod., 5, 71-78.
61. Pryor, J.L., Kent-First, M., Muallem, A. et al. (1997) Microdeletions in the Y chromosome of infertile men. N. Engl. J. Med., 336, 534-539.
62. 2+ channels and the acrosome reaction: which channels are present and what do they do? Hum. Reprod., 14, 873-879.
63. Richter, W., Dettmer, D. and Glander, H.-J. (1999) Detection of mRNA transcripts of cyclic nucleotide phosphodiesterase subtypes in ejaculated human spermatozoa. Mol. Hum. Reprod., 5, 732-736.
64. 1 integrins in ejaculated human spermatozoa by nested reverse transcription-polymerase chain reaction. Mol. Hum. Reprod., 2, 499-505.
65. Saiki, R.K., Scharf, S., Faloona, F. et al. (1985) Enzymatic amplification of B-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350-1354.
66. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbor Publications, Cold Spring Harbor, New York, USA.
67. 2+ rise during the acrosome reaction of mammalian spermatozoa induced by zona pellucida. Dev. Biol., 208, 70-78.
68. Snutch, T.P., Tomlinson, W.J., Leonard, J.P. et al. (1991) Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNS. Neuron, 7, 45-57.
69. Society for Assisted Reproductive Technology and the American Society for Reproductive Medicine (1998) Assisted reproductive technology in the United States and Canada: 1995 results from the American Society for Reproductive Medicine/Society for Assisted Reproductive Technology Registry. Fertil. Steril., 69, 389-398.
70. 2+ channel. Genomics, 22, 77-87.
71. Spungin, B. and Breitbart, H. (1996) Calcium mobilization and influx during sperm exocytosis. J. Cell, Sci., 109, 1947-1955.
72. 2+-selective channels in planar lipid bilayers. Am. J. Physiol., 268, C1284-C1294.
73. Tovey, S.C., Godfrey, R.E., Hughes, P.J. et al. (1997) Identification and characterization of inositol,1,4,5-triphosphate receptors in rat testis. Cell Calcium, 21, 311-319.
74. Tso, J.Y., Sun, X.H., Kao, T.H. et al. (1985) Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res., 13, 2485-2502.
75. Van Voorhis, B.J., Stovall, D.W., Allen, B.D. et al. (1998) Cost-effective treatment of the infertile couple. Fertil. Steril., 70, 995-1005.
76. Vassen, M. (1984) Childlessness and Infecundity. World Fertility Comparative Studies. Voorberg, The Netherlands: International Statistical Institute, p. 31.
77. Walensky, S.D. and Snyder, S.H. (1995) Inositol, 1,4,5-triphosphate receptors selectively localized to the acrosomes of mammalian sperm. J. Cell. Biol., 130, 857-869.
78. Walensky, L.D., Ruat, M., Bakin, R.E. et al. (1998) Two novel odorant receptor families expressed in spermatids undergo 5′-splicing. J. Biol. Chem., 273, 9378-9387.
79. Weissenberg, R., Aviram, A., Golan, R. et al. (1998) Concurrent use of flow cytometry and fluorescence in-situ hybridization techniques for detecting faulty meiosis in a human sperm sample. Mol. Hum. Reprod., 4, 61-66.
80. Welch, J.E., Schatte, E.C., O'Brien, D.A. et al. (1992) Expression of a glyceraldehyde-3-phosphate dehydrogenase gene specific to mouse spermatogenic cells. Biol. Reprod., 46, 869-878.
81. Westenbroek, R.E. and Babcock, D.F. (1999) Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. Dev. Biol. 207, 457-469.
82. Witkin, S.S., Korngold, G.C. and Bendich, A. (1975) Ribonuclease-sensitive DNA synthesizing complex in human sperm heads and seminal fluid. Proc. Natl. Acad. Sci. USA, 75, 3295-3299.
83. 2+ channel currents in undifferentiated mammalian NG108-15 cells. J. Physiol., 510, 347-360.
84. Wykes, S.M., Visscher, D.W. and Krawetz, S.A. (1997) Haploid transcripts persist in mature human spermatozoa. Mol. Hum. Reprod., 3, 15-19.