Detection of mRNA transcripts of β1 integrins in ejaculated human spermatozoa by nested reverse transcription-polymerase chain reaction

Molecular Human Reproduction

Volumn 2, Issue 7, 1996, Pages 499-505

  Rohwedder, Angela ^a   Liedigk, Oliver ^a   Schaller, Jörg ^b   Glander, Hans Jürgen ^c   Werchau, Hermann ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b St Barbara Hospital   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Human spermatozoa; mRNA of 1 integrins; Nested RT PCR; 1 integrins

Indexed keywords

ACTIN; BETA1 INTEGRIN; DNA; MESSENGER RNA; PRIMER DNA;

ARTICLE; CHEMISTRY; EJACULATION; GENETICS; HUMAN; MALE; METHODOLOGY; POLYMERASE CHAIN REACTION; SPERMATOZOON;

ACTINS; ANTIGENS, CD29; DNA; DNA PRIMERS; EJACULATION; HUMANS; MALE; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPERMATOZOA;

EID: 0030178052     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/2.7.499     Document Type: Article

References (38)

1. Argraves, W.S., Suzuki, S., Arai, H. et al. (1987) Amino acid sequence of the human fibronectin receptor. J. Cell Biol., 105, 1183-1190.
2. Blobel, C.P., Wolfsberg, T.G., Turck, C.W. et al. (1992) A potential fusion peptide and an integrin ligand domain in a protein active sperm-egg fusion. Nature, 356, 248-252.
3. Bronson, R.A. and Fusi, E. (1990) Evidence that an arg-gly-asp adhesion sequence plays a role in mammalian fertilization. Biol. Reprod., 43, 1019-1025.
4. Chiang, M.H., Steuerwald, N., Lambert, H. et al. (1994) Detection of human leukocyte antigen class I messenger ribonucleic acid transcripts in human spermatozoa via reverse transcription-polymerase chain reaction. Fertil. Steril., 61, 276-280.
5. Fusi, F.M., Vignali, M., Busacca, M. and Bronson, R.A. (1992) Evidence for the presence of an integrin cell adhesion receptor on the oolemma of unfertilized human oocytes. Mol. Reprod. Develop., 31, 215-222
6. Gatewood, J.M., Cook, G.R., Balhorn, R. et al. (1987) Sequence-specific packaging of DNA in human sperm chromatin. Science, 236, 962-996.
7. Glander, H.J. and Schaller, J. (1993) Beta 1 integrins of spermatozoa, a flow cytophotometric analysis. Int. J. Androl., 16, 105-111.
8. Glander, H.J. and Schaller, J. (1996) Disintegration of spermatozoal membranes in seminal plasma decreases the binding capacity of integrins. Andrologia, 28, 35-41.
9. Glander, H.J., Hermann, K. and Haustein, U.F. (1987) The equatorial fibronectin band (EFB) on human spermatozoa-a diagnostic help for male fertility? Andrologia, 19, 456-459.
10. Glander, H.J., Schaller, J., Weber, W. et al. (1996) In vitro fertilization: increased VLA (very late antigen) integrins and fibronectin after acrosome reaction. Arch. Androl., 36, 177-185.
11. Grandchamp, B., de Verneuil, H., Beaumont, C. et al. (1987) Tissue-specific expression of porphobilinogen deaminase. Two isoenzymes from a single gene. Eur. J. Biochem., 162, 105-110.
12. Hemler, M.E., Ware, C.F. and Strominger, J.L. (1983) Characterization of a novel differentiation antigen complex recognized by a monoclonal antibody (A-1A5): unique activation-specific molecular forms on stimulated T cells. J. Immunol., 132, 3011-3018.
13. Hemler, M.E., Sanchez-Madrid, F., Flotte, T.J. et al. (1984) Glycoproteins of 210,000 and 130,000 M.W. on activated T Cells: Cell distribution and antigenic relation to components on resting cells and T cell lines. J. Immunol., 132, 3011-3018.
14. Hynes, R. O. (1987) Integrins: a family of cell surface receptors. Cell, 48, 549-554.
15. Jackson, D.P., Hayden, J.D. and Quirke, P. (1991) Chapter 3. Extraction of nucleic acid from fresh and archival material 29-50. In McPherson, M.J., Quirke, P. and Taylor, G.R. (eds), PCR: A Practical Approach. Oxford University Press, New York.
16. 1 integrin cell adhesion molecules and fertilizing ability of human spermatozoa. Mol. Hum. Reprod., 1,
17. see Hum. Reprod., 10, 728-733.
18. Koehler, J. K., Nudelman, E. D. and Hakomori, S. (1980) A collagen-binding protein on the surface of ejaculated rabbit spermatozoa. J. Cell Biol., 86, 529-536.
19. Kumar, G., Patel, D. and Naz, R.K. (1993) c-MYC mRNA is present in human sperm cells. Cell. Mol. Biol. Res., 39, 111-117.
20. Marx, J.L. (1989) New family of adhesion proteins discovered. Science, 243, 1144.
21. Miller, D., Tang, P.Z., Skinner, C. and Lilford, R. (1994) Differential RNA fingerprinting as a tool in the analysis of spermatozoal gene expression. Hum. Reprod., 9, 864-869.
22. 1 integrin subunit in rat seminiferous epithelium. Biol. Reprod., 47, 1173-1182.
23. Quinn, P., Kerin, J.F. and Warnes, G.M. (1985) Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of the human tubal fluid. Fertil. Steril., 44, 493-498.
24. Ruoslahti, E. and Pierschbacher, M. D. (1987) New perspectives in cell adhesion. RGD and integrins. Science, 238, 491-497.
25. 1 integrins and fibronectin on spermatogenic cells in human testis. Hum. Reprod., 8, 1873-1878.
26. Schaller, J., Henkel, R., Glander, H.J. and Schill, W.B. (1995) Investigation of adhesion molecules and matrix proteins on human sperm with different binding and penetration properties in the HOP-test. Arch. Dermatol. Res., 287, 360.
27. Shimizu, H., Shimizu, C. and Burns, J.C. (1994) Detection of novel RNA viruses: morbilliviruses as a model system. Mol. Cell. Probes, 8, 209-214.
28. Spirin, A.S. (1994) Storage of messenger RNA in eukaryotes: envelopment with protein, translational barrier at 5' side, or conformational masking by 3′ side? Mol. Reprod. Develop., 38, 107-117.
29. Springer, T.A. (1990) Adhesion receptors of the immune system. Nature, 346, 425-434.
30. Suarez, S., Redfern, K., Raynor, P. et al. (1991) Attachment of boar sperm to mucosal explants of oviduct in vitro: possible role in formation of a sperm reservoir. Biol. Reprod., 44, 998-1004.
31. Sundström, D. and Nilsson, K. (1976) Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer, 17, 565-577.
32. Takada, Y., Elices, M.J., Crouse, C. and Hemler, M.E. (1989) The primary structure of the α4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function. EMBO J., 8, 1361-1368.
33. 4. J. Cell. Biol., 111, 1593-1604.
34. 3. J. Biochem., 109, 659-665.
35. Vuento, M., Kuusela, R., Virkki, M. and Koskimies, A. ( 1984) Characterization of fibronectin on human spermatozoa. Hoppe-Seyler's Z. Physiol. Chem., 365, 757-762.
36. Weinberg, R. (1992) The retinoblastoma gene and gene product. Cancer Surv., 12, 43-57.
37. Williams, A.F. (1987) A year in the life of the immunoglobulin superfamily. Immunol. Today, 8, 298-303.
38. World Health Organization (1993) Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 3rd edn. Cambridge University Press, Cambridge.