Induction of a high-affinity ketanserin binding site at the 5-Hydroxytryptamine(1B) receptor by modification of its carboxy-terminal intracellular portion

Biochemical Pharmacology

Volumn 59, Issue 9, 2000, Pages 1117-1121

  Wurch, Thierry ^a   Palmier, Christiane ^a   Pauwels, Petrus J ^a  

^a INSTITUT DE RECHERCHE PIERRE FABRE   (France)

Author keywords

5 HT(2A) receptors; Chimeric receptor; Ketanserin; Ligand binding; Recombinant 5 HT(1B)

Indexed keywords

2,3,6,7 TETRAHYDRO 1' METHYL 5 [[2' METHYL 4' (5 METHYL 1,2,4 OXADIAZOL 3 YL) 4 BIPHENYLYL]CARBONYL]SPIRO[FURO[2,3 F]INDOLE 3,4' PIPERIDINE]; CHIMERIC PROTEIN; DONITRIPTAN; KETANSERIN; N [4 METHOXY 3 (4 METHYL 1 PIPERAZINYL)PHENYL] 3 METHYL 4 (4 PYRIDYL)BENZAMIDE; RADIOLIGAND; RITANSERIN; SEROTONIN; SEROTONIN 1B RECEPTOR; SEROTONIN 2A RECEPTOR; SUMATRIPTAN; ZOLMITRIPTAN;

AMINO ACID COMPOSITION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL STRAIN COS1; GENE CONSTRUCT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BINDING SITES; CELLS, CULTURED; COS CELLS; HUMANS; KETANSERIN; LIGANDS; RECEPTOR, SEROTONIN, 5-HT1B; RECEPTOR, SEROTONIN, 5-HT2A; RECEPTORS, SEROTONIN; RECOMBINANT FUSION PROTEINS; SEROTONIN ANTAGONISTS;

EID: 0033969394     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(99)00416-5     Document Type: Article

References (15)

1. 2C receptors . Br J Pharmacol. 115:1995;622-628.
2. 1D receptor sites. Cell Pharmacol. 2:1995;183-191.
3. 1D receptors. Neuropharmacology. 36:1997;513-524.
4. 1D receptor subfamily . J Neurochem. 68:1997;410-418.
5. 1B/D receptor ligands An answer from GTPγS binding studies with stably transfected C6-glial cell lines . Neuropharmacology. 36:1997;499-512.
6. 1D receptor subtype. Mol Pharmacol. 40:1991;1036-1042.
7. 1D receptor Importance of the second extracellular loop and fifth transmembrane domain in antagonist binding . Mol Pharmacol. 54:1998;1088-1096.
8. Wurch T., Lestienne F., Pauwels P.J. A modified overlap extension PCR method to create chimeric genes in the absence of restriction enzymes. Biotechnol Techniques. 12:1998;653-657.
9. 1B receptors . Naunyn Schmiedebergs Arch Pharmacol. 353:1996;144-156.
10. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
11. Hurley J.H., Bloem L.J., Pavalko F., Liu J., Tian M., Simon J.R., Yu L. Structure-function studies of the eighth hydrophobic domain of a serotonin receptor. J Neurochem. 72:1999;413-421.
12. 2-adrenergic receptors based upon characteristics in primary structure. J Biol Chem. 267:1992;8342-8346.
13. 2C receptors . Med Chem Res. 3:1993;317-334.
14. 2C receptor interactions. Eur J Pharmacol. 306:1996;195-210.
15. Hofmann K and Stoffel W, TMbase - A database of membrane-spanning protein segments. J Biol Chem374: 166-, 1993.