Expression of a new operon from Bacillus subtilis, ykzB-ykoL, under the control of the TnrA and PhoP-PhoR global regulators

Journal of Bacteriology

Volumn 182, Issue 5, 2000, Pages 1226-1231

  Robichon, Denis ^a   Arnaud, Maryvonne ^a   Gardan, Rozenn ^a,d   Pragai, Zoltan ^b   O'Reilly, Mary ^c   Rapoport, Georges ^a   Débarbouillé, Michel ^a  

^a INSTITUT PASTEUR   (France)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c TRINITY COLLEGE DUBLIN   (Ireland)

^d CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMIC ACID; NITROGEN; PEPTIDE; PHOSPHATE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BINDING SITE; CULTURE MEDIUM; DNA SEQUENCE; GENE EXPRESSION REGULATION; GENE INACTIVATION; NITROGEN FIXATION; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; SEQUENCE HOMOLOGY;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GLUTAMIC ACID; LAC OPERON; MOLECULAR SEQUENCE DATA; OPERON; PHOSPHATES; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 0033965778     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.5.1226-1231.2000     Document Type: Article

References (30)

1. Amory, A., F. Kunst, E. Aubert, A. Klier, and G. Rapoport. 1987. Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J. Bacteriol. 169:324-333.
2. Atkinson, M. R., and S. H. Fisher. 1991. Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J. Bacteriol. 173:23-27.
3. Calogero, S., R. Gardan, P. Glaser, J. Schweizer, G. Rapoport, and M. Débarbouillé. 1994. RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators. J. Bacteriol. 176:1234-1241.
4. 54 from Gram-negative bacteria. Proc. Natl. Acad. Sci. USA 88:9092-9096.
5. Débarbouillé, M., R. Gardan, M. Arnaud, and G. Rapoport. 1999. Role of BkdR, a transcriptional activator of the SigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis. J. Bacteriol. 181:2059-2066.
6. Derré, I., G. Rapoport, K. Devine, M. Rose, and T. Msadek. 1999. ClpE, a novel type of HSP100 ATPase, is part of the CtsR heat shock regulon of Bacillus subtilis. Mol. Microhiol. 32:581-593.
7. Eder, S., W. Liu, and F. M. Hulett. 1999. Mutational analysis of the phoD promoter in Bacillus subtilis: implications for PhoP binding and promoter activation of Pho regulon promoters. J. Bacteriol. 181:2017-2025.
8. Ferson, A. E., L. V. Wray, Jr., and S. H. Fisher. 1996. Expression of the Bacillus subtilis gabP gene is regulated independently in response to nitrogen and amino acid availability. Mol. Microbiol. 22:693-701.
9. Fisher, S. H. 1999. Regulation of nitrogen metabolism in Bacillus subtilis: vive la différence! Mol. Microhiol. 32:223-232.
10. Hu, P., T. Leighton, G. Ishkhanova, and S. Kustu. 1999. Sensing of nitrogen limitation by Bacillus subtilis: comparison to enteric bacteria. J. Bacteriol. 181:5042-5050.
11. Hulett, F. M., C. Bookstein, and K. Jensen. 1990. Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis. J. Bacteriol. 172:735-740.
12. Kunst, F., T. Msadek, J. Bignon, and G. Rapoport. 1994. The DegS/DegU and ComP/ComA two-component systems are part of a network controlling degradative enzyme synthesis and competence in Bacillus subtilis. Res. Microbiol. 145:393-402.
13. Kunst, F., N. Ogasawara, I. Moszer, A. M. Albertini, G. Alloni, et al. 1997. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390:249-256.
14. Liu, W., and F. M. Hulett. 1998. Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho-regulon promoters establishes a Bacillus subtilis Pho core binding site. Microbiology 144:1443-1450.
15. Martin-Verstraete, I., M. Débarbouillé, A. Klier, and G. Rapoport. 1992. Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J. Mol. Biol. 226:85-99.
16. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Msadek, T., V. Dartois, F. Kunst, M.-L. Herbaud, F. Denizot, and G. Rapoport. 1998. ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. Mol. Microbiol. 27:899-914.
18. Mullis, K. B., and F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350.
19. Nakano, M. M., F. Yang, P. Hardin, and P. Zuber. 1995. Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis. J. Bacteriol. 177:573-579.
20. Nakano, M. M., T. Hoffman, Y. Zhu, and D. Jahn. 1998. Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE. J. Bacteriol. 180:5344-5350.
21. Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, et al. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491.
22. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
24. Schreier, H. J., S. W. Brown, K. D. Hirschi, J. F. Nomellini, and A. L. Sonenshein. 1989. Regulation of Bacillus subtilis glutamine synthetase gene expression by the product of the glnR gene. J. Mol. Biol. 210:51-63.
25. Serror, P., and A. L. Sonenshein. 1996. Interaction of CodY, a novel Bacillus subtilis DNA-binding protein, with the dpp promoter region. Mol. Microbiol. 20:843-852.
26. Tabor, S., and C. C. Richardson. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84:4767-4771.
27. Vagner, V., E. Dervyn, and S. D. Ehrlich. 1998. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144:3097-3104.
28. II protein. J. Bacteriol. 176:108-114.
29. Wray, L. V., Jr., A. E. Ferson, K. Rohrer, and S. H. Fisher. 1996. TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 93:8841-8845.
30. Wray, L. V., Jr., J. M. Zalieckas, and S. H. Fisher. 1998. Mutational analysis of the TnrA-binding sites in the Bacillus subtilis nrgAB and gabP promoter regions. J. Bacteriol. 180:2943-2949.