Mutational analysis of the phoD promoter in Bacillus subtilis: Implications for PhoP binding and promoter activation of Pho regulon promoters

Journal of Bacteriology

Volumn 181, Issue 7, 1999, Pages 2017-2025

  Eder, Steve ^a   Liu, Wei ^a,b   Hulett, F Marion ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DIMER; PHOP PROTEIN; PROTEIN PHOR; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BINDING SITE; CONTROLLED STUDY; DNA DETERMINATION; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE INSERTION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; REACTION ANALYSIS; REGULATORY MECHANISM; REGULON; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0032911194     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.7.2017-2025.1999     Document Type: Article

References (30)

1. Chesnut, R. S., C. Bookstein, and F. M. Hulett. 1991. Separate promoters direct expression of phoAIII, a member of the Bacillus subtilis alkaline phosphatase multigene family, during phosphate starvation and sporulation. Mol. Microbiol. 5:2181-2190.
2. Cutting, S. M., and J. A. Vander Horn. 1990. Genetic analysis, p. 27-74. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for bacillus. John Wiley, New York, N.Y.
3. Eder, S., L. Shi, K. Jensen, K. Yamane, and F. M. Hulett. 1996. A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD. Microbiology 142:2041-2047.
4. Ferrari, E., S. Howard, and J. A. Hoch. 1986. Effect of stage 0 sporulation mutants on subtilisin expression. J. Bacteriol. 166:173-179.
5. Hanahan, D. 1985. Techniques for transformation of E. coli. p. 109-135. In D. M. Glover (ed.), DNA cloning II: a practical approach. IRL Press, Washington, D.C.
6. Harlocker, S. L., L. Bergstrom, and M. Inouye. 1995. Tandem binding of six OmpR proteins to the ompF upstream regulatory sequence of Escherichia coli. J. Biol. Chem. 270:26849-26856.
7. Hulett, F. M. 1995. Complex phosphate regulation by sequential switches in Bacillus subtilis, p. 289-302. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. American Society for Microbiology, Washington, D.C.
8. Hulett, F. M., C. Bookstein, and K. Jensen. 1990. Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis. J. Bacteriol. 172:735-740.
9. Hulett, F. M., E. E. Kim, C. Bookstein, N. Kapp, C. W. Edwards, and H. W. Wycoff. 1991. Bacillus subtilis alkaline phosphatase III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three dimensional structure. J. Biol. Chem. 266:1348-1358.
10. Hulett, F. M., J. Lee, L. Shi, G. Sun, R. Chesnut, and E. Sharkova. 1994. Sequential action of two-component genetic switches regulates the Pho regulon in Bacillus subtilis. J. Bacteriol. 176:1348-1358.
11. Hulett, F. M., G. Sun, and W. Liu. 1994. The Pho regulon of Bacillus subtilis is regulated by the sequential action of two genetic switches, p. 50-54. In A. Torriani-Gorini, E. Yogil, and S. Silver (ed.), Phosphate in microorganisms: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
12. Jensen, K., E. Sharkova, M. F. Duggan, Y. Qi, A. Koide, J. A. Hoch, and F. M. Hulett. 1993. Bacillus subtilis transcription regulator, Spo0A, decreases alkaline phosphatase levels induced by phosphate starvation. J. Bacteriol. 175:3749-3756.
13. Lang, W. K., K. Glassy, and A. R. Archibald. 1982. Influence of phosphate supply on teichoic acid and teichuronic acid content of Bacillus subtilis cell walls. J. Bacteriol. 151:367-375.
14. Liu, W., S. Eder, and F. M. Hulett. 1998. Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P. J. Bacteriol. 180:753-758.
15. Liu, W., and F. M. Hulett. 1997. Bacillus subtilis PhoP binds to the phoB tandem promoter exclusively within the phosphate starvation-inducible promoter. J. Bacteriol. 179:6302-6310.
16. Liu, W., and F. M. Hulett. 1998. Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho regulon promoters establishes a Bacillus subtilis Pho core binding site. Microbiology 144:1443-1450.
17. Liu, W., Y. Qi, and F. M. Hulett. 1998. Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity. Mol. Microbiol. 28:119-130.
18. Makino, K., M. Amemura, T. Kawamoto, S. Kimura, H. Shingawa, A. Nakata, and M. Suzuki. 1996. DNA binding of PhoB and its interaction with RNA polymerase. J. Mol. Biol. 259:15-26.
19. Makino, K., H. Shinagawa, M. Amemura, S. Kimura, A. Nakata, and A. Ishihama. 1988. Regulation of the phosphate regulon of Escherichia coli, activation of pstS transcription by PhoB protein in vitro. J. Mol. Biol. 203: 85-95.
20. Martinez-Hackert, E., and A. M. Stock. 1996. The DNA-binding domain of OmpR: crystal structure of a winged helix transcription factor. Structure 5:109-124.
21. Martinez-Hackert, E., and A. M. Stock. 1997. Structural relationships in the OmpR family of winged-helix transcription factors. J. Mol. Biol. 269:301-312.
22. Parkinson, J. S. 1993. Signal transduction schemes of bacteria. Cell 73:857-871.
23. Qi, Y., and F. M. Hulett. 1998. PhoP-P and RNA polymerase sigmaA holoenzyme are sufficient for transcription of Pho regulon promoters in Bacillus subtilis: PhoP-P activator sites within the coding region stimulate transcription in vivo. Mol. Microbiol. 28:1187-1197.
24. Qi. Y., Y. Kobayashi, and F. M. Hulett. 1997. The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the Pho regulon. J. Bacteriol. 179:2534-2539.
25. Shimotsu, H., M. I. Kuroda, C. Yanofsky, and D. J. Henner. 1986. Novel form of transcription attenuation regulates expression of the Bacillus subtilis tryptophan operon. J. Bacteriol. 166:461-471.
26. Stock, J. B., A. J. Ninfa, and A. M. Stock. 1984. Protein phosphorylation and regulation of adaptive response regulators in bacteria. Microbiol. Rev. 53: 450-490.
27. Stock, J. B., A. M. Stock, and J. M. Mottenen. 1990. Signal transduction in bacteria. Nature 344:395-400.
28. Sun, G., S. M. Birkey, and F. M. Hulett. 1996. Three two-component signal-transduction systems interact for Pho regulation in Bacillus subtilis. Mol. Microbiol. 19:941-948.
29. Yamane, K., and B. Maruo. 1978. Alkaline phosphatase possessing alkaline phosphodiesterase activity and other phosphodiesterases in Bacillus subtilis. J. Bacteriol. 134:108-114.
30. Yamane, K., and B. Maruo. 1978. Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis. J. Bacteriol. 134: 100-107.