The dprA gene is required for natural transformation of Helicobacter pylori

FEMS Immunology and Medical Microbiology

Volumn 27, Issue 2, 2000, Pages 99-102

  Smeets, Leonard C ^a   Bijlsma, Jetta J E ^a   Kuipers, Ernst J ^b   Vandenbroucke Grauls, Christina M J E ^a   Kusters, Johannes G ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Genetic transformation; Helicobacter pylori; Suicide plasmid; Transformation deficient mutant

Indexed keywords

BACTERIAL DNA; PLASMID DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM COMPETENCE; BACTERIUM MUTANT; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; GENE CONTROL; GENE INSERTION; NONHUMAN; PRIORITY JOURNAL; STRAIN DIFFERENCE;

BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; HELICOBACTER PYLORI; MEMBRANE PROTEINS; MUTATION; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 0033963597     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(99)00170-4     Document Type: Article

References (19)

1. Suerbaum S., Smith J.M., Bapumia K., Morelli G., Smith N.H., Kunstmann E., Dyrek I., Achtman M. Free recombination within Helicobacter pylori. Proc. Natl. Acad. Sci. USA. 95:1998;12619-12624.
2. Owen R.J., Bickley J., Hurtado A., Fraser A., Pounder R.E. Comparison of PCR-based restriction length polymorphism analysis of urease genes with rRNA gene profiling for monitoring Helicobacter pylori infections in patients on triple therapy. J. Clin. Microbiol. 32:1994;1203-1210.
3. Kersulyte D., Chalkauskas H., Berg D.E. Emergence of recombinant strains of Helicobacter pylori during human infection. Mol. Microbiol. 31:1999;31-43.
4. Censini S., Lange C., Xiang Z., Crabtree J.E., Ghiara P., Borodovsky M., Rappuoli R., Covacci A. Cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA. 93:1996;14648-14653.
5. Taylor N.S., Fox J.G., Akopyants N.S., Berg D.E., Thompson N., Shames B., Yan L., Fontham E., Janney F., Hunter F.M., et al. Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting. J. Clin. Microbiol. 33:1995;918-923.
6. Nedenskov-Sorensen P., Bukholm G., Bovre K. Natural competence for genetic transformation in Campylobacter pylori. J. Infect. Dis. 161:(2):1990;365-366.
7. Kuipers E.J., Israel D.A., Kusters J.G., Blaser M.J. Evidence for a conjugation-like mechanism of DNA transfer in Helicobacter pylori. J. Bacteriol. 180:1998;2901-2905.
8. Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C., Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D., Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature. 397:1999;176-180.
9. Tomb J.F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.P., Gill S., Dougherty B.A., Nelson K., Quackenbush J., Zhou L., Kirkness E.F., Peterson S., Loftus B., Richardson D., Dodson R., Khalak H.G., Glodek A., McKenney K., Fitzegerald L.M., Lee N., Adams M.D., Venter J.C. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature. 388:1997;539-547.
10. Hofreuter D., Odenbreit S., Henke G., Haas R. Natural competence for DNA transformation in Helicobacter pylori-identification and genetic characterization of the ComB locus. Mol. Microbiol. 28:1998;1027-1038.
11. Bijlsma J.J.E., Vandenbroucke-Grauls C.M., Phadnis S.H., Kusters J.G. Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis. Infect. Immun. 67:1999;2433-2440.
12. Heuermann D., Haas R. A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation. Mol. Gen. Genet. 257:1998;519-528.
13. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. Wang Y., Roos K.P., Taylor D.E. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. J. Gen. Microbiol. 139:1993;2485-2493.
15. Debets-Ossenkopp Y.J., Sparrius M., Kusters J.G., Kolkman J.J., Vandenbroucke-Grauls C.M.J.E. Mechanism of clarithromycin resistance in clinical isolates of Helicobacter pylori. FEMS Microbiol. Lett. 142:1996;37-42.
16. Morrison D.A., Lacks S.A., Guild W.R., Hageman J.M. Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination. J. Bacteriol. 156:1983;281-290.
17. Palmen R., Vosman B., Buijsman P., Breek C.K., Hellingwerf K.J. Physiological characterization of natural transformation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 139:1993;295-305.
18. Tomb J.F., Barcak G.J., Chandler M.S., Redfield R.J., Smith H.O. Transposon mutagenesis, characterization, and cloning of transformation genes of Haemophilus influenzae Rd. J. Bacteriol. 171:1989;3796-3802.
19. Karudapuram S., Zhao X., Barcak G.J. DNA sequence and characterization of Haemophilus influenzae DprA+, a gene required for chromosomal but not plasmid DNA transformation. J. Bacteriol. 177:1995;3235-3240.