The yeast Chs4 protein stimulates the trypsin-sensitive activity of chitin synthase 3 through an apparent protein-protein interaction

Microbiology

Volumn 146, Issue 2, 2000, Pages 385-391

  Ono, Naomi ^a   Yabe, Tomio ^a,b   Sudoh, Masayuki ^a   Nakajima, Tasuku ^b   Yamada Okabe, Toshiko ^c   Arisawa, Mikio ^a   Yamada Okabe, Hisafumi ^a  

^a NIPPON ROCHE RESEARCH CENTER   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Chitin synthase 3; Overexpression; Protein protein interaction; Yeast

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; CHITIN SYNTHASE; CHOLESTEROL HYDROGEN SUCCINATE; TRYPSIN; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

ARTICLE; ENZYME ACTIVITY; GENE OVEREXPRESSION; NONHUMAN; PRENYLATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING;

EID: 0033953795     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-2-385     Document Type: Article

References (31)

1. Bulawa, C. E. (1992). CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD1 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis. Mol Cell Biol 12, 1764-1776.
2. Bulawa, C. E. (1993). Genetics and molecular biology of chitin synthesis in fungi. Annn Rev Microbiol 47, 505-534.
3. Bulawa, C. E., Slater, M., Cabib, E., Au-Young, J., Sburlati, A., Adair, W. L., Jr & Robbins, P. W. (1986). The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell 46, 213-225.
4. Cabib, E., Sburlati, A., Bowers, B. & Silverman, S. J. (1989). Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J Cell Biol 108, 1655-1672.
5. Cabib, E., Silverman, S. J. & Shaw, J. A. (1992). Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Saccharomyces cerevisiae. J Gen Microbiol 138, 97-102.
6. Cabib, E., Shaw, J. A., Mol, P. C, Bowers, B. & Choi, W.-J. (1996). In The Mycota, vol. III, Biochemistry and Molecular Biology, pp. 243-267. Edited by R. Brambl & G. A. Marzluf. Berlin, Heidelberg & New York: Springer.
7. Choi, W.-J., Sburlati, A. & Cabib, E. (1994). Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL3 genes. Proc Natl Acad Sci USA 91, 4727-1730.
8. Chuang, J. S. & Schekman, R. W. (1996). Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p. J Cell Biol 135, 597-610.
9. Cos, T., Ford, R., Trilla, A., Duran, A., Cabib, E. & Roncero, C. (1998). Molecular analysis of Chs3p participation in chitin synthase III activity. Eur J Biochem 256, 419-426.
10. DeMarini, D. J., Adams, A. E. M., Fares, H., Virgilio, C. D., Valle, G., Chuang, J. S. & Pringle, J. R. (1997). A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J Cell Biol 139, 75-93.
11. Feilotter, H. E., Hannon, G. J., Ruddell, C J. & Beach, D. (1994). Construction of an improved host strain for two hybrid screening. Nucleic Acids Res 22, 1502-1503.
12. Holthuis, J. C. M., Nichols, B. J. & Pelham, H. R. B. (1998). The syntaxin Tlg1p mediates trafficking of chitin synthase III to polarized growth sites in yeast. Mol Biol Cell 9, 3383-3397.
13. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983). Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153, 163-168.
14. Kawamoto, S., Nomura, M. & Ohno, T. (1992). Cloning and characterization of SKT5, a Saccharomyces cerevisiae: gene that affects protoplast regulation and resistance to killer toxin of Kluyveromyces lactis. J Ferment Bioeng 74, 199-208.
15. Kollar, R., Petrakova, E., Ashwell, G., Robbins, P. W. & Cabib, E. (1995). Architecture of the yeast cell wall. The linkage between chitin and beta (1→3)-glucan. J Biol Chem 270, 1170-1178.
16. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
17. Nagahashi, S., Sudoh, M., Ono, N. & 7 other authors (1995). Characterization of chitin synthase 2 of Saccharomyces cerevisiae: implication of two highly conserved domains as possible catalytic sites. J Biol Chem 270, 13961-13967.
18. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn, pp. 18.60-18.75. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
19. Santos, B. & Snyder, M. (1997). Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p. J Cell Biol 136, 95-110.
20. Santos, B., Duran, A. & Valdivieso, M. H. (1997). CHS5, a gene-involved in chitin synthesis and mating in Saccharomyces cerevisiae. Mol Cell Biol 17, 2485-2496.
21. Sburlati, A. & Cabib, E. (1986). Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae. J Biol Chem 261, 15147-15152.
22. Shaw, J. A., Mol, P. C., Bowers, B., Silverman, S. J., Valdivieso, M. H., Duran, A. & Cabib, E. (1991). The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae: cell cycle. J Cell Biol 114, 111-123.
23. Silverman, S. J. (1989). Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: two isozymes with distinct functions. Yeast 5, 459-467.
24. Silverman, S. J., Sburlati, A., Slater, M. L. & Cabib, E. (1988). Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 85, 4735-4739.
25. Sudoh, M., Tatsuno, K., Ono, N., Chibana, H., Yamada-Okabe, H. & Arisawa, M. (1999). The Candida albicans CHS4 gene complements a Saccharomyces cerevisiae: skt5/chs4 mutation and is involved in chitin biosynthesis. Microbiology 145, 1613-1622.
26. Takita, M. & Castilho-Valvicius, B. (1993). Absence of cell wall chitin in Saccharomyces cerevisiae: leads to resistance to Kluyveromyces lactis killer toxin. Yeast 9, 589-598.
27. Trilla, J. A., Cos, T., Duran, A. & Roncero, C. (1997). Characterization of CHS4 (CAL2), a gene of Saccharomyces cerevisiae: involved in chitin biosynthesis and allelic to SKT5 and CSD4. Yeast 13, 795-807.
28. Valdivieso, M. H., Mol, P. C., Shaw, J. A., Cabib, E. & Duran, A. (1991). CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J Cell Biol 114, 101-109.
29. Yabe, T., Yamada-Okabe, T., Nakajima, T., Sudoh, M., Arisawa, M. & Yamada-Okabe, H. (1998). Mutational analysis of chitin synthase 2 of Saccharomyces cerevisiae identification of additional amino acid residues involved in the catalysis. Eur J Biochem 258, 941-947.
30. Ziman, M., Chuang, J. S. & Schekman, R. W. (1996). Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway. Mol Biol Cell 7, 1909-1919.
31. Ziman, M., Chung, J. S., Tsung, M., Hashimoto, S. & Schekman, R. (1998). Chs6p-dependent anterograde transport of Chs3p from the chitosome to the plasma membrane in Saccharomyces cerevisiae. Mol Biol Cell 9, 1565-1576.